Rad18 (NM_001167730) Mouse Untagged Clone

CAT#: MC218972

Rad18 (untagged) - Mouse RAD18 homolog (S. cerevisiae) (Rad18), transcript variant 1, (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Rad18
• Synonyms: 2810024C04Rik; Rad18sc
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC218972 representing NM_001167730
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAGGTCCTGGCCGAGCCGCGATGGCCTCCGGGGTTGGCGGTGATGAAGACAATAGATGACTTGCTGC
  GCTGTGGGATTTGCTTTGAGTATTTCAACATTGCAGTGATAATCCCCCAGTGCTCTCACAACTATTGTTC
  ACTCTGTATAAGAAAGTTTTTATCCTATAAAACTCAGTGCCCAACTTGTTGCGTGGCAGTAACGGAGCCA
  GACCTGAGAAATAATCGCCTCTTAGATGAACTGGTAAAAAGCATGAATTTTGCACGGACTCACCTGTTGC
  AGTTTGCTTTAGAGTCACCACCCATATCTCCTGTGTCTTCCACCTCAAAGAAGGTTGTTGTTAAAGTGCA
  TAATGCTGACGCCGCCCAACACCCTGTCAAACAGGCGAACAGGTTAATGGATAAGTTCCTGATTAGAGAA
  ACTGGTGACTGTGTATTTGAGTTGTTGGGAAAAGAAAATGAGAGGAAATTCAGCCCTCAAAAAGAGCTAA
  GCACCTCTGCTGAGATTAAAGAGACAAGTCTCCTAGGAAAGCCGGTACTGGGGCTCTCGGATGCTAATGG
  TCCTGTGACTCCCTCTACATCCACTATGAAACTGGATACTAAAGTGTCTTGTCCTGTTTGTGGGGTCAGC
  ATTCCAGAAAATCATATCAATAAGCATTTAGACAGTTGTTTATCACGTGAAGAGAAGAAGGAGAGCCTGC
  GAAGTTCTGCTCACAAAAGGAAGCCGTTGCCCAAAACTGTATATAACTTGCTCTCTGATCGTGATTTAAA
  GAAAAAGCTGAAACAGTATGGCTTATCTGTTCAAGGAAACAAACAGCAGCTTATTAAAAGGCATCAGGAG
  TTTGTGCACATGTATAATGCCCAGTGCGATGCTTTGCATCCTAAATCAGCTGCTGAAATCGTCCAAGAAA
  TTGAAAGCATGGAGAAGACCAGGATGCGCCTTGAAGCAAGTAAACTCAATGAAAATGTCATGGTTTTTAC
  AAAGAACCAAACAGAGAAGGAAATTGAGGAAGTTCACAGTGAATATCATGCTGGCAAACAGGTAGCACAG
  GGCTGTGTGAGCGAAAACAAGATGAGAAGCACCATAGTCTGCTGGCAGTTATCAGGTAAAAAGCACCAGA
  ATGCATTCCAGCTTCTGGTGGATCAGGCCAAAAAAGGGTATAAGAAAACTGGCAGAGTTTCACAAGCTGC
  AGCGATGAGAACAGATGAACCTGCAGAGACACTGCCGTCGATGAGAACAGATGAACCTGCAGAGACACTG
  CCGTCGATGAGAACAGATGAACCTGCAGAGACACTGCCGTTGATGAGAGCAGATGAACCTGCAGAAACAC
  TGCCGTCTGAGTGTATCGCACAAGAAGATAATGTGAGCTTCTCAGATACTGTCTCAGTAACAAACCACTT
  TCCTCAGCCCCAGCTGGACTCGCCAGGCCCGTCGGAGCCTGAGAGGCCGGATGATTCTTCTAGTTGTACT
  GATATTCTTTTCTCCTCGGACTCAGACTCATGCAATAGTTCCAGTTCAGACATCATAAGAGATCTTCTGG
  AAGAAGAGGAAGCCTGGGAAGCGGCTCACAAAAATGATCAAAACAGAGAAGTCAGCCCACAACAGACTCG
  CCGCACTAGAGCCAGTGAATGTGTTGAGATTGAACCAAGAAACAAGCGGAATAAGAATTAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001167730
• Insert Size: 1671 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001167730.1, NP_001161202.1
• RefSeq Size: 2701 bp
• RefSeq ORF: 1671 bp
• Locus ID: 58186
• Cytogenetics: 6 E3
• Gene Summary: E3 ubiquitin-protein ligase involved in postreplication repair of UV-damaged DNA. Postreplication repair functions in gap-filling of a daughter strand on replication of damaged DNA. Associates to the E2 ubiquitin conjugating enzyme UBE2B to form the UBE2B-RAD18 ubiquitin ligase complex involved in mono-ubiquitination of DNA-associated PCNA on 'Lys-164'. Has ssDNA binding activity.[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.