Gm21949 (NM_001113419) Mouse Untagged Clone

CAT#: MC218969

Gm21949 (untagged) - Mouse IQ motif containing J-schwannomin interacting protein 1 readthrough (Iqcj-schip1), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Gm21949
• Synonyms: Iqcj-schip1; Schip-1-iqcj; Schip1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC218969 representing NM_001113419
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCGACTGGAAGAACTAAAGAGACTACAGAATCCTTTAGAACAAGTTGACGATGGAAAATATTTGCTTG
  AGAATCACCAGCTGGCCATGGATGTGGAGAATAACATTGAGAACTATCCCCTCAGCCTACAGCCCTTGGA
  ATCAAAGGTGAAAATCATCCAGCGAGCATGGCGAGAGTACTTGCAGAGGCAGGACCCCCTGGAGAAGCGG
  AGCCCATCCCCACCTTCTGTCTCTTCAGACAAGCTGAGCAGCTCTGTCAGCATGAACACCTTCTCCGACA
  GCAGCACACCCGATTACCGAGAGGATGGGATGGATCTAGGCAGTGACGCCGGCAGCAGCAGCAGCAGCCG
  CGCCAGCTCACAATCCAACTCCACCAAAGTGACCCCTTGCTCCGAGTGCAAATCCTCATCGTCGCCGGGG
  GGTAGCTTGGACTTGGTGTCTGCTCTGGAAGACTATGAAGAGCCCTTCCCGGTCTACCAGAAGAAGGTGA
  TTGATGAGTGGGCTCCGGAGGAGGACGGGGAGGAGGAGGAAGAGGAGGACGATCGAGGGTATCGGGATGA
  CGGCTGTCCGGCCCGGGAGCCGGGGGACGTGAGCGCCAGGATCGGCAGCAGCGGCAGCGGGAGCAGGAGC
  GCCGCCACCACCATGCCGTCCCCGATGCCTAACGGCAACCTCCATCCGCACGACCCTCAAGACCTCAGGC
  ACAATGGCAACGTGGTGGTAGCTGGCCGGCCGAACGCCTCTCGGGTTCCCCGGCGACCCATCCAGAAGAC
  CCAGCCTCCAGGGAGCCGGCGCGGAGGCCGAAACCGGGCATCTGGGGGCCTCTGCCTTCAGCCACCGGAC
  GGCGGGACGCGGGTCCCGGAAGAACCCCCTGCGCCCCCAATGGACTGGGAGGCATTGGAAAAGCATCTGG
  CCGGGCTGCAGTTCCGGGAGCAGGAGGTGCGAAACCAGGGTCAGGCGAGGACCAACTCCACCTCTGCACA
  GAAGAATGAGAGAGAGTCTATCAGACAGAAGTTGGCACTCGGAAGCTTCTTTGACGATGGCCCAGGAATC
  TATACCAGCTGCAGCAAAAGTGGGAAGCCAAGCCTTTCTGCAAGACTACAGAGCGGGATGAACCTCCAGA
  TATGCTTTGTCAATGACAGCGGCAGTGACAAGGACAGCGATGCAGATGACAGTAAGACGGAAACCAGCTT
  GGACACGCCCTTGTCCCCCATGAGCAAGCAGAGTTCTTCCTATTCGGATAGAGACACAACTGAGGAGGAG
  TCTGAATCCCTGGATGACATGGACTTCCTCACAAGGCAAAAGAAGCTACAAGCTGAAGCCAAAATGGCTC
  TGGCCATGGCCAAACCAATGGCCAAAATGCAAGTAGAAGTGGAAAAACAGAACAGGAAAAAGTCTCCCGT
  CGCTGATCTTCTCCCACACATGCCTCACATAAGCGAATGTTTGATGAAAAGAAGCTTAAAGCCCACCGAC
  CTGAGAGACATGACTATCGGGCAGCTACAAGTGATCGTCAATGACCTCCACTCCCAGATTGAAAGTTTGA
  ATGAAGAGTTGGTCCAGCTGCTCCTTATTCGAGATGAGCTGCACACAGAACAAGATGCCATGCTGGTGGA
  CATTGAAGACTTGACTAGACACGCTGAGAGTCAGCAGAAGCACATGGCTGAGAAAATGCCCGCGAAGTGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001113419
• Insert Size: 1680 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001113419.2, NP_001106890.1
• RefSeq Size: 2397 bp
• RefSeq ORF: 1680 bp
• Locus ID: 100505386
• UniProt ID: A0A088MLT8
• Cytogenetics: 3
• Gene Summary: This locus represents naturally occurring read-through transcription from the neighboring IQ motif containing J (Iqcj) and schwannomin interacting protein 1 (Schip1) genes. This locus produces a transcript that is composed of in-frame exons from each individual gene. The resulting fusion protein is thought to be a component of the multimolecular complexes of axon initial segments and nodes of Ranvier, and it may play a role in calcium-mediated responses. [provided by RefSeq, Oct 2010]