Nars (NM_027350) Mouse Untagged Clone

CAT#: MC218924

Nars (untagged) - Mouse asparaginyl-tRNA synthetase (Nars), transcript variant 2, (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Nars
• Synonyms: 3010001M15Rik; AA960128; ASNRS; C78150
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC218924 representing NM_027350
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTCCTCGGAGGTGATTAGGGGGACTGCAGAGATGGTTCTAGAGCTGTATGTATCTGACCGAGAAGGAA
  ATGACGCAACCGGGGATGGAACCAAGGAGAAACCATTTAAAACAGGCCTAAAGGCTTTGATGACAGTTGG
  AAAAGAGCCATTTCCCACCATTTATGTGGACTCACAAAAGGAAAATGAGAGATGGGATGTTATTTCTAAG
  TCACAGATGAAGAACATTAAAAAGATGTGGCACAGAGAACAGATGAAGAACGACTCTAGGGAGAAGAAAG
  AGGCAGAAGATAACTTACGAAGAGAAAAGAACCTGGAGGAAGCAAAAAAAATTATTATTAAAAATGACCC
  GAGCCTGCCAGAGCCGGCCTGTGTAAAGATTAGTGCATTAGAAGGATACAGAGGCCAGAGAGTGAAGGTG
  TTTGGCTGGGTCCACAGGTTGCGCAGGCAAGGAAAGAATTTGATGTTTTTGGTGTTGCGAGATGGTACGG
  GTTATCTTCAGTGTGTCTTGTCAGATGACTTGTGTCAGTGTTACAATGGAGTAGTCCTGTCCACCGAGAG
  TAGTGTGGCGGTGTACGGAACACTAAACCTAACTCCAAAGGGCAAACAGGCTCCAGGAGGCCATGAGCTG
  AGCTGTGACTTCTGGGAACTGGTGGGGCTGGCCCCGGCTGGAGGAGCTGATAACCTGATCAACGAGGAGT
  CTGATGTGGATGTCCAGCTCAACAACCGGCACATGATGATCCGGGGAGAGAACATGTCCAAAATCCTGAA
  AGCGCGCTCCATGATCACCAGGTGTTTCCGGGACCACTTCTTCGACAGGGGCTACTGTGAAGTTACCACT
  CCAACACTGGTGCAGACGCAGGTGGAAGGTGGGGCCACGCTCTTCAAGCTTGACTATTTCGGGGAAGAAG
  CGTTTTTGACCCAGTCCTCACAGTTGTACCTGGAGACCTGCCTTCCAGCCCTGGGAGATGTTTTTTGTAT
  AGCCCAGTCATACAGGGCCGAACAGTCCAGGACACGAAGGCACCTGGCTGAGTTCACTCACGTGGAAGCT
  GAGTGTCCTTTCCTGACCTTCGAGGACCTCCTGAACCGTTTAGAGGACCTAGTGTGTGATGTGGTGGACA
  GAGTCCTGAAGTCACCAGTGGCAAGCATAGTGTATGAGCTCAACCCGAACTTTAAACCCCCCAAGCGGCC
  TTTCCGCAGGATGAACTACTCGGATGCTATTGAGTGGCTGAAGGAGCATGATGTCAAGAAAGAAGACGGG
  ACTTTCTACGAGTTTGGAGATGATATTCCCGAAGCGCCTGAGAGACTGATGACAGACACCATTAATGAAC
  CAATCCTGCTGTGTCGCTTTCCTGTGGAGATCAAGTCTTTCTATATGCAGCGCTGTCCTGAGGATCCTCG
  ACTTACTGAATCTGTGGACGTGTTGATGCCCAACGTTGGTGAGATTGTAGGTGGCTCGATGCGCTCCTGG
  GACAGTGAGGAGATTCTCGAAGGCTATAAGAGGGAAGGGATTGACCCCGCTCCTTACTACTGGTATACAG
  ATCAGAGAAAATATGGCACCTGTCCTCATGGAGGGTATGGCTTGGGCTTGGAACGATTTCTTAGCTGGAT
  TCTGAACAGGTATCACATCCGAGATGTGTGCCTGTACCCTCGATTTCTCCAGCGCTGCAGGCCATAA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_027350
• Insert Size: 1677 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_027350.3, NP_081626.2
• RefSeq Size: 2660 bp
• RefSeq ORF: 1677 bp
• Locus ID: 70223
• UniProt ID: Q8BP47
• Cytogenetics: 18 E1
• Gene Summary: Catalyzes the attachment of asparagine to tRNA(Asn) in a two-step reaction: asparagine is first activated by ATP to form Asn-AMP and then transferred to the acceptor end of tRNA(Asn). In addition to its essential role in protein synthesis, acts as a signaling molecule that induces immune response in a CCR3-dependent manner.[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (2) uses an alternate in-frame splice site in the 5' coding region, compared to variant 1. The resulting isoform (2) is 1 aa shorter than isoform 1.