Cbx4 (NM_007625) Mouse Untagged Clone

CAT#: MC218791

Cbx4 (untagged) - Mouse chromobox homolog 4 (Drosophila Pc class) (Cbx4), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Cbx4
• Synonyms: MPc2; PC2
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC218791 representing NM_007625
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAGCTGCCAGCTGTTGGCGAGCACGTCTTCGCGGTGGAGAGCATCGAAAAGAAGCGGATACGCAAGG
  GCAGAGTGGAGTATCTGGTGAAATGGAGAGGCTGGTCCCCCAAATATAACACGTGGGAACCAGAGGAGAA
  CATCTTGGATCCCCGGCTGCTGATCGCCTTCCAGAACAGGGAAAGGCAGGAGCAGCTGATGGGATATCGC
  AAGAGAGGGCCCAAGCCCAAACCGCTGGTGGTGCAGGTGCCCACCTTTGCCCGACGCTCCAATGTTCTGA
  CTGGGCTTCAAGACTCCTCCGCTGACAACCGTGCCAAGCTGGAGTTGGGCACACAGGGCAAGGGCCAGGG
  GCACCAGTATGAGCTCAACAGCAAGAAGCACCATCAGTACCAGCCACATAGCAAGGAGAGGTCCGGTAAG
  CCGCCCCCGCCAGGGAAGAGCGGCAAGTATTATTATCAGCTAAATAGCAAAAAGCACCACCCCTACCAGC
  CAGACCCCAAAATGTACGACCTGCAGTACCAGGGCGGCCACAAGGAGGCACCCAGCCCCACCTGCCCAGA
  CCTGGGCACCAAGAGCCACCCTCCTGACAAGTGGGCCCACGGAGCTGCAGCCAAAGGCTACCTCGGGGCC
  GTGAAGCCCTTGGGGGGAGGGGCAGGAGCTCCAGGCAAGGGCTCGGAAAAGGGCCCCCCCAATGGCATGA
  CACCAGCCCCTAAGGAGGCAGTGACCGGTAATGGTATTGGGGGCAAGATGAAAATCGTCAAGAACAAGAA
  CAAGAATGGGCGCATCGTGATCGTTATGAGCAAGTACATGGAGAACGGCATGCAGGCAGTGAAAATCAAG
  TCGGGCGAGGCCGCCGAGGGCGAGGCGCGCTCCCCCAGCCACAAGAAACGTGCTGCCGAGGAGCGCCATC
  CCCAGGGCGACAGGACTTTTAAAAAGGCAGCCGGTGCCTCTGAGGAGAAGAAAGCCGAAGTGCCCTGCAA
  ACGCAGGGAGGAGGAGGCTCTGGTGTCGGGGGACGCGCAGCCCCAGGACCTAGGGTCTCGAAAGCTCTCC
  CCGACCAAGGAGGCCTTTGGTGAGCAACCGCTACAGCTCACCACCAAGCCAGACCTGCTGGCCTGGGACC
  CAGCCAGGAGCTCGCACCCGCCCGCCCACCACCACCATCACCACCACCATCACCACCACCACCATACGGT
  GGGCCTGAACCTCTCCCATGCGCGCAAGCGCTGCCTCTCCGAGACCCATGGGGAGCGTGAGCCCTGTAAG
  AAGCGGTTGACCGCCCGCAGCATCAGCACGCCGACCTGCCTGGGGGGCAGCCCGGTCTCCGAGCACCCTG
  CCAACGTATCCCCCACCGCGGCCTCTCTGCCGCAGCCCGAGGTCATCCTTTTAGACTCAGACCTGGATGA
  ACCCATAGACTTGCGCTGTGTCAAGATGCGCAGCGACGCCGGGGAACCGCCCAGCACCCTCCAGGTGAAG
  CCCGAGGCTCCAGCTGTGGCGGCAGTGGTGGCGCCGGCCCCTGCCTCAGAGAAGCCTCCCGCTGAGGCCC
  AGGAGGAGCCAGTGGAGCCCTTGAGTGAGTTCAAGCCCTTCTTTGGGAATATAATTATCACTGACGTCAC
  GGCGAACTGCCTTACCGTCACTTTCAAGGAATACGTGACGGTGTAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_007625
• Insert Size: 1656 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_007625.2, NP_031651.2
• RefSeq Size: 5213 bp
• RefSeq ORF: 1656 bp
• Locus ID: 12418
• UniProt ID: O55187
• Cytogenetics: 11 83.34 cM
• Gene Summary: E3 SUMO-protein ligase which facilitates SUMO1 conjugation by UBE2I. Involved in the sumoylation of HNRNPK, a p53/TP53 transcriptional coactivator, hence indirectly regulates p53/TP53 transcriptional activation resulting in p21/CDKN1A expression.[UniProtKB/Swiss-Prot Function]