Has2 (NM_008216) Mouse Untagged Clone

CAT#: MC218773

Has2 (untagged) - Mouse hyaluronan synthase 2 (Has2), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Has2
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC218773 representing NM_008216
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCATTGTGAGAGGTTTCTATGTGTCCTGAGAATAATTGGAACTACACTTTTTGGAGTGTCTCTCCTCC
  TCGGAATCACAGCTGCTTATATTGTTGGCTACCAGTTTATCCAAACAGATAATTACTACTTCTCATTTGG
  ACTGTACGGTGCCTTTTTAGCCTCGCATCTCATCATCCAAAGCCTCTTTGCCTTTTTGGAACACCGGAAA
  ATGAAGAAGTCCCTTGAAACCCCGATTAAATTGAACAAAACGGTAGCACTCTGCATCGCTGCGTACCAAG
  AGGACCCTGACTACTTACGGAAATGTTTGCAATCTGTGAAAAGGCTGACCTACCCTGGGATTAAAGTCGT
  GATGGTCATCGATGGGAACTCAGACGACGACCTTTACATGATGGACATATTCAGCGAAGTTATGGGCAGG
  GACAAATCGGCCACGTACATCTGGAAGAACAACTTTCATGAAAAGGGACCTGGTGAGACAGAAGAGTCCC
  ATAAAGAAAGTTCACAACATGTCACCCAATTGGTCTTGTCTAACAAAAGTATTTGCATCATGCAAAAATG
  GGGTGGAAAGAGAGAAGTCATGTACACAGCCTTCAGAGCACTGGGGCGAAGCGTGGATTATGTACAGGTG
  TGTGACTCAGATACTATGCTTGACCCTGCCTCATCTGTGGAGATGGTGAAGGTCTTAGAGGAAGACCCTA
  TGGTTGGAGGTGTTGGAGGAGATGTCCAGATTTTAAACAAGTATGATTCCTGGATCTCCTTCCTCAGCAG
  CGTGAGATACTGGATGGCTTTTAATATAGAAAGGGCCTGCCAGTCTTATTTTGGCTGTGTCCAGTGCATA
  AGCGGTCCTCTGGGAATGTACAGAAACTCCTTGCTGCATGAATTTGTGGAAGACTGGTACAATCAGGAAT
  TCATGGGTAACCAATGCAGTTTTGGTGACGACAGGCACCTTACCAACAGGGTGTTGAGTCTGGGCTATGC
  AACTAAATACACGGCTCGGTCCAAGTGCCTTACTGAAACTCCCATAGAATATCTGAGATGGCTGAACCAG
  CAGACCCGTTGGAGCAAGTCCTACTTCCGAGAGTGGCTGTACAATGCCATGTGGTTTCACAAGCATCACT
  TGTGGATGACCTATGAAGCTGTTATCACTGGATTCTTTCCTTTCTTTCTCATTGCCACAGTCATCCAGCT
  CTTCTACAGGGGTAAAATCTGGAACATCCTCCTCTTCCTGTTAACTGTCCAGCTAGTGGGTCTCATCAAG
  TCATCTTTTGCCAGCTGCCTTAGAGGAAATATCGTCATGGTATTCATGTCTCTGTATTCAGTGTTATACA
  TGTCAAGTCTACTTCCTGCCAAGATGTTTGCAATTGCAACCATAAACAAAGCTGGGTGGGGCACATCTGG
  AAGGAAGACCATTGTTGTTAATTTCATAGGACTTATTCCAGTGTCCGTGTGGTTTACAATCCTTCTAGGT
  GGTGTAATTTTCACCATTTATAAGGAATCTAAAAAGCCATTTTCCGAATCCAAACAGACTGTTCTCATCG
  TGGGAACTTTGATCTATGCATGCTACTGGGTCATGCTTTTGACTCTCTATGTGGTTCTCATCAATAAGTG
  TGGCAGGCGGAAGAAGGGACAACAGTATGACATGGTGCTTGATGTATGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_008216
• Insert Size: 1659 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_008216.3, NP_032242.3
• RefSeq Size: 4262 bp
• RefSeq ORF: 1659 bp
• Locus ID: 15117
• UniProt ID: P70312
• Cytogenetics: 15 23.31 cM
• Gene Summary: Catalyzes the addition of GlcNAc or GlcUA monosaccharides to the nascent hyaluronan polymer. Therefore, it is essential to hyaluronan synthesis a major component of most extracellular matrices that has a structural role in tissues architectures and regulates cell adhesion, migration and differentiation. This is one of the isozymes catalyzing that reaction and it is particularly responsible for the synthesis of high molecular mass hyaluronan. Required for the transition of endocardial cushion cells into mesenchymal cells, a process crucial for heart development. May also play a role in vasculogenesis. High molecular mass hyaluronan also play a role in early contact inhibition a process which stops cell growth when cells come into contact with each other or the extracellular matrix.[UniProtKB/Swiss-Prot Function]