Stx5a (NM_001167799) Mouse Untagged Clone

CAT#: MC218742

Stx5a (untagged) - Mouse syntaxin 5A (Stx5a), transcript variant 2, (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Stx5a
• Synonyms: 0610031F24Rik; D19Ertd627e; Stx5
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC218742 representing NM_001167799
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGATCCCGCGGAAACGCTACGGATCTAAGAACACGGATCAGGGTGTCTACCTGGGTCTCTCAAAGACAC
  AGGTTCTGTCCCCTGCAACTGCTATCAGTAGCAGCAGTGACAGCACTCCTTTGCCCACTCCGGTGGCCCT
  GGTCCCCTCCCCTCCCGACACCATGTCCTGCCGGGATCGGACCCAGGAGTTCCAGTCTGCCTGCAAGTCC
  CTGCAGAGCCGTCAGAATGGAATCCAAACAAGCAAACCGGCTCTGCACGCAGCCCGACAGTGCAGTGAAT
  TTACCCTCATGGCCAGGCGCATTGGGAAAGATCTCAGCAATACATTTGCCAAGCTGGAGAAGCTAACAAT
  CTTGGCAAAGCGCAAGTCCCTCTTTGATGATAAAGCAGTAGAAATTGAGGAGCTAACATACATCATCAAA
  CAGGATATCAATAGCCTCAACAAACAAATTGCTCAGCTTCAAGATTTTGTGAGGGCCAAGGGCAGCCAGA
  GTGGCCGGCATCTGCAGACCCATTCTAACACCATTGTGGTTTCGTTGCAGTCAAAGCTGGCTTCTATGTC
  CAATGACTTCAAGTCAGTTCTAGAAGTGAGGACTGAGAATCTGAAACAGCAGAGGAACCGTCGGGAACAG
  TTCTCCAGGGCACCAGTGTCAGCCCTGCCTCTTGCCCCCAACAACCTTGGAGGTGGTCCCATAATTTTGG
  GAGCAGAGTCCCGAGCTTCCAGGGACGTGGCTATTGACATGATGGACCCTAGAACAAGCCAGCAGCTTCA
  GCTCATTGATGAGCAGGATTCCTACATCCAGAGTCGGGCAGACACCATGCAGAACATTGAGTCTACAATC
  GTTGAGCTGGGCTCCATTTTTCAGCAATTGGCACACATGGTTAAAGAACAGGAGGAAACGATTCAGAGGA
  TCGACGAGAATGTGCTTGGAGCCCAGCTGGATGTGGAGGCAGCCCATTCAGAGATCCTCAAGTACTTCCA
  GTCAGTTACCTCCAATCGGTGGCTCATGGTCAAAATCTTCCTCATCCTCATTGTCTTCTTCATCATCTTT
  GTGGTCTTCCTTGCCTGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001167799
• Insert Size: 1068 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001167799.1, NP_001161271.1
• RefSeq Size: 1829 bp
• RefSeq ORF: 1068 bp
• Locus ID: 56389
• UniProt ID: Q8K1E0
• Cytogenetics: 19 A
• Gene Summary: Mediates endoplasmic reticulum to Golgi transport. Together with p115/USO1 and GM130/GOLGA2, involved in vesicle tethering and fusion at the cis-Golgi membrane to maintain the stacked and inter-connected structure of the Golgi apparatus.[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (2) differs in the 5' UTR compared to variant 1. Both variants encode the same protein. CCDS Note: he coding region has been updated to extend the N-terminus to one that is supported by available conservation data. The use of an alternative upstream start codon would result in a protein that is 54 aa longer.