Ppp1r16b (NM_001159662) Mouse Untagged Clone

CAT#: MC218604

Ppp1r16b (untagged) - Mouse protein phosphatase 1, regulatory (inhibitor) subunit 16B (Ppp1r16b), transcript variant 1, (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Ppp1r16b
• Synonyms: ANKRD4; C130078N17Rik; TIMAP; Wdt4
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC218604 representing NM_001159662
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCCAGCCACGTGGACCTGCTCACAGAGCTGCAGCTGCTGGAGAAGGTGCCTACGCTGGAGCGCCTGC
  GCGCAGCCCAGAAGCGCCGGGCTCAGCAGCTGAAGAAGTGGGCACAGTACGAGCAGGACCTGCTTCATCG
  CAAGCGCAAGCATGAGCGGAAGCGCAGCACGGGTGGACGTCGCAAGAAGGTGTCCTTCGAGGCCAGTGTA
  GCGTTGCTGGAAGCCTCACTGAGGAATGACGCCGAAGAAGTTCGCTACTTCCTGAAGAACAAGGTTAGCC
  CTGATTTGTGCAATGAGGATGGACTTACAGCCTTGCACCAGTGCTGCATTGACAACTTTGAAGAGATTGT
  CAAGCTGCTGCTGTCCCACGGTGCGAACGTGAATGCCAAGGACAACGAGCTGTGGACGCCCTTGCACGCG
  GCAGCCACCTGCGGCCACATCAACCTGGTGAAGATCCTCGTTCAGTATGGGGCTGACTTGCTTGCTGTCA
  ACTCTGATGGAAACATGCCATATGACCTCTGCGAGGATGAACCCACCCTGGATGTCATCGAGACCTGCAT
  GGCATACCAAGGCATCACACAGGAGAAAATCAACGAGATGCGAGCAGCCCCTGAGCAGAAGATGATTTCA
  GACATCCACTGCATGATAGCAGCGGGCCAGGATCTCGACTGGATCGATGGCCAGGGTGCCACACTGCTAC
  ACATAGCTGGAGCCAATGGATATCTGCGAGCAGCCGAGCTCCTCCTGGACCATGGAGTGCGTGTGGATGT
  GAAGGACTGGGATGGCTGGGAGCCTCTGCATGCAGCAGCCTTCTGGGGACAGATGCCAATGGCAGAGCTG
  CTGGTGTCCCATGGAGCTAGTCTCAGTGCCAGGACATCCATGGATGAGATGCCAATAGACCTGTGTGAGG
  AGGAGGAATTCAAGGTCCTGCTGTTGGAGCTGAAACACAAGCATGATGTGATCATGAAGTCGCAGCTGAG
  GCACAAGTCGTCCCTGAGCCGGAGGACATCCAGCGCGGGCAGCCGTGGGAAGGTGGTGCGTCGAGCCAGC
  CTGTCAGACAGAACCAACCTATACCGGAAAGAATATGAGGGCGAGGCCATCTTGTGGCAGCAGCGGAGTG
  CAGCTGAGGACCAGCGGACTTCAACCTACAATGGAGACATCAGGGAGACCAGGACAGACCAGGAGAACAA
  GGACCCGAACCCCAGGCTGGAGAAGCCCGTGCTGCTCTCAGAATTCTCTACCAAGATCTCACGGGGAGAA
  CTGGATGGGCCTGTGGAGAATGGCCTCCGGGCTCCTGTCAGCACTTACCAGTATGCGCTGGCCAATGGGG
  ACATATGGAAGATGCACGAGATGCCTGACTACAGCATGGCCTACGGCAACCCTGGCGTGGCTGATGTCCC
  CCCACCCTGGAGTGGCTTCAAGGAGCAGAGCCCCCAAACGCTCCTGGAGCTGAAGAGGCAGAGGGCCGCA
  GCCAAGCTGCTCAGTCACCCATTCTTAAGCACCCACCTGGGCAGCAGCGTGGCCAGGTCGGGGGAGAGCA
  GCAGCGAAGGCAAGGCCCCCTTGATTGGAGGCAGAACTTCCCCCTACAGCAGCAACGGGACCTCGGTGTA
  TTATACAGTCACCAGCGGAGATCCCCCGCTCTTGAAGTTCAAGGCCCCCATGGAGGAGATGGAGGAGAAA
  GTTCACGGCTGCTGCCGGATCTCCTAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001159662
• Insert Size: 1707 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001159662.1, NP_001153134.1
• RefSeq Size: 6392 bp
• RefSeq ORF: 1707 bp
• Locus ID: 228852
• UniProt ID: Q8VHQ3
• Cytogenetics: 2 H1
• Gene Summary: Regulator of protein phosphatase 1 (PP1) that acts as a positive regulator of pulmonary endothelial cell (EC) barrier function. Protects the endothelial barrier from lipopolysaccharide (LPS)-induced vascular leakage (PubMed:21907835). Involved in the regulation of the PI3K/AKT signaling pathway (By similarity). Involved in the regulation of angiogenesis and endothelial cell proliferation through the control of ECE1 dephosphorylation, trafficking and activity (By similarity). Involved in the regulation of endothelial cell filopodia extension (By similarity). May be a downstream target for TGF-beta1 signaling cascade in endothelial cells (By similarity). Involved in PKA-mediated moesin dephosphorylation which is important in EC barrier protection against thrombin stimulation. Promotes the interaction of PPP1CA with RPSA/LAMR1 and in turn facilitates the dephosphorylation of RPSA/LAMR1 (By similarity). Involved in the dephosphorylation of EEF1A1 (By similarity).[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (1) represents the longer transcript. Both variants encode the same protein. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.