Rbbp5 (NM_172517) Mouse Untagged Clone

CAT#: MC218569

Rbbp5 (untagged) - Mouse retinoblastoma binding protein 5 (Rbbp5), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Rbbp5
• Synonyms: 4933411J24Rik; C330016J05
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC218569 representing NM_172517
  Red=Cloning site Blue=ORF
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAACCTCGAGTTGCTGGAGTCCTTTGGACAGAACTACCCAGAGGAAGCTGATGGGACTCTGGACTGCA
  TCAGCATGGCCCTGACCTGCACCTTTAACAGGTGGGGCACACTGCTTGCTGTTGGCTGTAACGATGGCCG
  GATTGTTATTTGGGATTTCTTGACCAGAGGCATTGCTAAAATAATTAGTGCACACATCCATCCAGTCTGT
  TCTTTGTGTTGGAGCCGAGATGGTCATAAGCTTGTGAGTGCTTCTACAGATAACATAGTGTCACAGTGGG
  ATGTTCTTTCAGGAGATTGCGACCAGAGGTTTCGATTCCCTTCACCCATCTTAAAAGTCCAATATCATCC
  TCGAGATCAGAACAAGGTTCTCGTGTGTCCCATGAAATCTGCTCCTGTCATGTTGACCCTTTCAGATTCC
  AAACATGTTGTTCTGCCGGTAGACGATGACTCGGATTTGAACGTGGTGGCATCTTTTGATAGGCGAGGGG
  AATATATTTATACAGGAAATGCAAAAGGCAAGATCTTGGTCCTAAAAACAGACTCTCAGGATCTTGTTGC
  TTCCTTCAGAGTAACAACTGGGACAAGCAATACTACCGCCATTAAATCAATTGAGTTTGCCCGGAAGGGG
  AGTTGCTTCCTGATTAACACAGCAGATCGAATAATAAGAGTCTATGACGGCAGAGAGATTTTAACGTGTG
  GAAGGGATGGAGAGCCAGAGCCTATGCAGAAGCTGCAGGACTTGGTGAATAGGACTCCATGGAAAAAATG
  TTGTTTCTCTGGGGATGGGGAGTACATAGTGGCGGGCTCTGCGAGGCAGCATGCGCTGTATATCTGGGAA
  AAGAGCATTGGCAACCTGGTGAAGATCTTACATGGGACCAGAGGGGAACTCCTGCTGGACGTGGCTTGGC
  ATCCAGTCCGACCCATCATAGCTTCTATCTCTAGTGGAGTGGTGTCCATTTGGGCCCAAAATCAAGTAGA
  AAATTGGAGTGCATTTGCACCAGACTTCAAAGAGTTGGATGAAAATGTAGAATATGAGGAAAGAGAATCA
  GAATTTGATATTGAGGATGAAGATAAGAGTGAGCCTGAGCAAACAGGGGCTGATGCTGCTGAAGATGAGG
  AAGTGGATGTCACCAGCGTGGATCCCATCGCTGCCTTCTGTAGCAGTGATGAAGAGCTGGAAGATTCAAA
  GGCTCTATTGTATTTACCCATTGCCCCTGAGGTAGAAGACCCTGAAGAAAACCCGTATGGCCCTCCACCG
  GATGCAGTCCCAAGCTCCTTGATGGATGAAGGGGCTAGTTCAGAGAAGAAGAGGCAGTCTTCAGCAGATG
  GATCCCAGCCACCAAAGAAGAAACCTAAAACCACCAATATAGAGCTCCAAGGAGTGCCGAATGATGAAGT
  CCATCCACTACTGGGTGTGAAGGGGGATGGCAAATCCAAGAAGAAGCAAGCAGGCCGGCCTAAAGGATCA
  AAAGGTAAAGAGAAAGATTCTCCATTTAAACCGAAACTCTACAAAGGGGACAGAGGTTTACCTCTGGAAG
  GATCAACGAAGGGTAAAGTGCAGGCGGAACTCAGCCAGTCCTTGGCAGCAGGAGGAGCCATCTCAGAACT
  GCTGTGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_172517
• Insert Size: 1617 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_172517.2, NP_766105.2
• RefSeq Size: 3632 bp
• RefSeq ORF: 1617 bp
• Locus ID: 213464
• UniProt ID: Q8BX09
• Cytogenetics: 1 E4
• Gene Summary: As part of the MLL1/MLL complex, involved in mono-, di- and trimethylation at 'Lys-4' of histone H3. Histone H3 'Lys-4' methylation represents a specific tag for epigenetic transcriptional activation. In embryonic stem (ES) cells, plays a crucial role in the differentiation potential, particularly along the neural lineage, regulating gene induction and H3 'Lys-4' methylation at key developmental loci, including that mediated by retinoic acid. Does not affect ES cell self-renewal.[UniProtKB/Swiss-Prot Function]