6720463M24Rik (BC052183) Mouse Untagged Clone

CAT#: MC218493

6720463M24Rik (untagged) - Mouse RIKEN cDNA 6720463M24 gene (cDNA clone MGC:59525 IMAGE:6336038), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: 6720463M24Rik
• Synonyms: 6720463M24Rik; AI317232
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >BC052183
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGGAGATGTCAGCGAATTAAAAATGCAAATAACACCAGAAACTCCAGGAAGGATCCCTGTTTTAAATC
  CCTTTGAAAGTCCCAGTGATTATTCTAATCTCCATGAACAAACCCTTGCTAGTCCTTCTATCTTTAAGTC
  CACAAAACTACCAACTCCAGGGAAATTTAGGTGGTCTATTGATCAGCTGGCCATAATAAACCCTGTAGAG
  ATCGATCCAGAGGAGATCCATCGGCAGGCTTCCTACCTAAGGCTTTCTCGAATAGATAAAGATGTGGAAG
  ACAAAAGACAAAAAGCCATTGAAGAGTTTTTTACTAAAGATGTCATTGTGCCCTCTCCCTGGACTGATCA
  TGATGGAAAACAGCCTTCAGAGCTTCATCCCAGTAAATGCCTAAGTAGCCATGATGACTCTCCTGATGGG
  AAAAAGCCAAGTCTTCCCTCACAGAAGTGCAACGCTGCCTGCCAGACTCTGCTGTCTCTTCCTGTGGATT
  TTAATTTGGAAGCCATATTAGGAGACTATTTTAGAGAAGAGGACTTTGTGGCCCACACTCCTGGGAACCT
  CAGTTCTTCATCTCTGAGAAGAAAGCTCTTTTTGGATGGGAATGGAAGCATCTGTGACCCTTTGCCTTCG
  CCGTCGCCCGGAAGTCCTCCCTGCAGTGCCCGGGGGTCGCTCGAGGGACAGTTTTCTTCCAGCCCCATTC
  AGAACAGTGTGAAAAAATACAGCTTGGGAAGCGTCACCACGAGCCCGTCGGCCATCTCCTCACCTACCTT
  CTCACCTATCGCACTTCAAGGAGGAAAGACGCCGCTCTCAGGTCAAATCTAA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCTGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: BC052183
• Insert Size: 822 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: BC052183, AAH52183
• RefSeq Size: 3866 bp
• RefSeq ORF: 821 bp
• Locus ID: 77744
• Cytogenetics: 14 E2.2
• Gene Summary: Required for the activation of AURKA at the onset of mitosis.[UniProtKB/Swiss-Prot Function]