Ddi2 (BC031491) Mouse Untagged Clone

CAT#: MC218404

Ddi2 (untagged) - Mouse DNA-damage inducible protein 2 (cDNA clone MGC:27896 IMAGE:3499157), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Ddi2
• Synonyms: 1110056G13Rik; 1700027M01Rik; 9130022E05Rik; AI604911; AU040698
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >BC031491
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTCATCATTGCCGACTTCAGATGGGTTTGACCATCCAGCTCCTTCAGGGCAGAGTCCTGAGGTTGGTA
  GCCCGACGAGTCTCGCTCGCTCTGTTTCTGCTTCCGTCTGCGCCATCAAGCCCGGTGACCCCAATAGCAT
  TGAATCTCTAGCTATGGAGGCTACGAAGGCTTCAGCTGAATTCCAGACAAACTCTAAGAAAACAGACCCT
  CCTCCTCTGCAGGTTCTTCCTGACCTTGCTTCCTCAGCAGAGCAGAGTCTAGCCATGCCTTTCCATAAGT
  CATCAAAAGAAGCCGTTGTTGCAGGTAATCTGGAGAAATCTGTTGAGAAAGGAACCCAGGGCCTCAGAGT
  GTATCTCCACACAAGACAGGACGCTAGTTTAACTCTCACAACTACTGGGATGCGGGAGCCACAGATATTT
  GCGGAGGAAAAGAGTTGGCATCCAGAAAATCAGACCCCAAGTCCCGTGAACGGCCTTCAGCAGCACAGAG
  AAACAGGGAGTGTACAGCGAGAGGCTGGACAGCAGAGTGTTCCACAGGACCAGGGCTGTCTTTGTGACGC
  AGAAGACCTTGAGCTTCATGAAGAAGTTGTCAGTTTGGAAGCTCTGAGGAAAGGTGAGCTACAAAGACAC
  GCTCATCTTCCCAGTGCAGAGAAGGGTCTTCCAGCTTCAGGACTCTGTAGCTGTCCATGCTCAGAAGCCC
  TGATGGAAGTAGATACAGCTGAACAGTCTCTGGTTGCTATGTGCAGCTCAACAGGCAGGCAGGATGCCGT
  CATCAAGAGCCCTAGTGTAGCACATCTCGCTTCAGATAATCCCACTATGGAAGTGGAGACATTACAGTCT
  AACCCGTCCTGTGAGCCTGTGGAACATTCCATCTTGACTCGGGAATTGCAGCTCCCAGAAGATAATGTTG
  ACATGTCTACAATGGATAACAAAGATGACAATTCCTCTTCCCTTCTAAGTGGCCACGGTCAGCCCTCTGT
  GGAATCAGCAGAAGAATTTTGTTCATCTGTCACAGTGGCCTTGAAAGAACTCCATGAGCTTTTGGTCATT
  AGCTGTAAGCCAGCTTCAGAAGAGTCACCTGAGCATGTTACCTGTCAGTCAGAGATAGGAGCTGAGAGCC
  AACCAAGTGTTTCAGACCTTTCAGGAAGAAGGGTCCAAAGTGTGCATTTGACCCCTAGTGACCAGTACTC
  ACAAGGCTCCTGTCACCAGGCCACCTCTGAATCAGGAAAGACAGAAATCGTAGGAACTGCCCCTTGTGCT
  GCGGTAGAAGATGAAGCATCCACTAGCTTTGAAGGTCTGGGTGATGGCTTGTCACCTGACCGAGAAGATG
  TCCGCAGATCAACAGAGTCAGCCAGGAAGAGCTGTTCTGTCGCCATAACCTCAGCTAAACTGTCTGAGCA
  GTTGCCCTGCACCTCAGGGGTAGAAATAGCACCTGAACTTGCAGCAAGTGAGGGTGCCCACAGTCAGCCT
  TCAGAGCATGTGCATAATCCAGGCCCAGACAGGCCGGAGACCAGCAGTGTCTGCCCTGGAGCTGGGTTGC
  CCCGTAGTGGATTGGACCAACCTCCCACACAGTCCTTGTCCACCCCCTCCGTTCTTCCACCGTTCATCTT
  TCCTGCTGCAGATGTTGACAGGATTCTTGGTGCCGGCTTCACTCTGCAGGAAGCGCTCGGGGCTCTGCAT
  CGAGTTGGTGGGAATGCAGACCTTGCCCTTCTTGTTTTGTTAGCAAAGAACATTGTAGTCCCAACATAA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCTGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: BC031491
• Insert Size: 1146 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: BC031491
• RefSeq Size: 2307 bp
• RefSeq ORF: 1748 bp
• Locus ID: 68817
• Cytogenetics: 4 E1
• Gene Summary: Aspartic protease that mediates the cleavage of NFE2L1/NRF1 at 'Leu-104', thereby promoting release of NFE2L1/NRF1 from the endoplasmic reticulum membrane. Ubiquitination of NFE2L1/NRF1 is a prerequisite for cleavage, suggesting that DDI2 specifically recognizes and binds ubiquitinated NFE2L1/NRF1. Seems to act as a proteasomal shuttle which links the proteasome and replication fork proteins like RTF2. Required, with DDI1, for cellular survival following replication stress. Together or redudantly with DDI1, removes RTF2 from stalled forks to allow cell cycle progression after replication stress and maintains genome integrity.[UniProtKB/Swiss-Prot Function]