Vps53 (BC034371) Mouse Untagged Clone

CAT#: MC218399

Vps53 (untagged) - Mouse vacuolar protein sorting 53 (yeast) (cDNA clone MGC:19253 IMAGE:3964008), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Vps53
• Synonyms: RP23-147P4.2
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >BC034371
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGATGGAGGAGGAGGAACTGGAGTTTGTGGAGGAGCTGGAGGCTGTGCTGCAGCTCACGCCAGAGGTGC
  AACTCGCCATCGAGCAGGTATTCCCAAGCCAGGATCCTCTTGATCAAGCTGACTTCAATGCCGTTGAGTA
  TATCAATACTCTGTTCCCAACAGAGCAATCTCTGGCAAACATTGACGATGTTGTGAACAAAATTAGACTG
  AAAATAAGGAGACTGGATGACAATATCCGGACTGTCGTAAGAGGTCAGACAAATGTGGGGCAGGATGGAA
  GGCAAGCACTTGAAGAGGCCCAGAAAGCCATTCAGCAACTCTTTGGCAAAATCAAGGATATCAAAGACAA
  AGCAGAAAAGTCAGAACAAATGGTGAAAGAGATCACCCGAGATATTAAGCAGTTAGATCACGCCAAGCGC
  CACCTGACCACCTCAATCACGACCCTGAACCACCTGCACATGCTGGCAGGCGGGGTGGACTCCCTGGAAG
  CCATGACCAGGCGCAGACAGTATGGAGAAGTTGCTAATCTCCTTCAGGGTGTAATGAATGTTCTGGAGCA
  CTTTCACAAATACATGGGAATCCCACAGATCCGACAGCTTTCTGAAAGGGTGAAGGCTGCACAGACAGAG
  TTAGGACAGCAAATCCTGGCTGACTTTGAAGAAGCGTTTCCTTCGCAGGGTACCAAGCCGAAGAAGCCCA
  AGGCCCCGGACAACCCGTTCCACGGCATCGTTTCCAAGTGTTTTGAGCCTCATCTCTACGTGTACATTGA
  GTCCCAGGACAAAAACCTCAGCGAGCTGATAGACCGGTTTGTGGCAGATTTCAAAGCCCAGGGCCCACCC
  AAGCCCAACACTGACGAAGGGGGTGCTGTGCTCCCCAGCTGCGCTGACCTCTTTGTCTACTACAAGAAGT
  GCATGGTGCAGTGCTCACAGCTCAGCACAGGCGAGCCCATGATCGCCCTGACTACCATCTTCCAGAAGTA
  CCTCCGAGAATACGCCTGGAAAATCCTCTCTGGCAACCTGCCTAAAACCAGCAGCAGCAGTGGGGGGCTG
  ACCATCAGCAGCCTCCTCAAAGAGAAGGAGGGCTCAGAAGTGGCCAGGTTCACTCTGGAGGAGCTGTGTC
  TCATCTGCAGCATCCTGAGCACAGCCGAGTATTGTCTGGCTACCACCCAGCAGCTAGAAGAAAAACTCAA
  AGAAAAAGTTGATGTGAGCCTGACTGAACGAATTAATCTCACAGGAGAAATGGACACATTCAGCACTGTC
  ATCTCCAGCAGCATCCAGCTGTTGGTTCAAGATCTGGATGCAGCCTGTGACCCTGCCCTGATTGCCATGA
  GCAAGATGCCTTGGCAGAACGTGGAGCATGTTGGTGACCAGAGCCCTTATGTCACTTCTGTTATTCTTCA
  CATTAAGCAAAACGTCCCCATCATCCGTGACAACCTGGCTTCCACACGGAAATACTTCACACAGTTCTGC
  ATTAAATTTGCAAATTCGTTCATTCCCAAATTCATCACCCACCTCTTCAAGTGTAAGCCGATCAGCATGG
  TGGGAGCAGAGCAGCTTCTGCTGGACACCCACTCCCTGAAGATGGTCCTGCTCGACCTTCCGTCCATTGG
  CTCCCAGGTGGTGAGGAAGGCCCCTGCCAGTTACACTAAGATTGTCGTGAAAGGCATGACCCGGGCTGAA
  ATGATCCTTAAGGTGGTGATGGCCCCTCATGAACCGTTGGTGGTATTTGTTGACAACTACATCAAACTCC
  TTACGGACTGCAACTCAGAAACCTTCCAGAAAATACTGGATATGAAGGGGCTGAAGAGGAGTGAGCAGAG
  CAGCATGCTGGAACTTTTGCGGCAGAGGCTCCCTGCCCCGCCCTCGGGGACAGAAGGTTCCAGCACCCTG
  TCGCTGATTGCCCCAACACCGGAGCAGGAGTCATCCCGAATCCGCAAACTGGAGAAACTGATTAAAAAGA
  GACTGTAA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCTGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: BC034371
• Insert Size: 1968 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: BC034371, AAH34371
• RefSeq Size: 2225 bp
• RefSeq ORF: 1967 bp
• Locus ID: 68299
• Cytogenetics: 11 B5
• Gene Summary: Acts as component of the GARP complex that is involved in retrograde transport from early and late endosomes to the trans-Golgi network (TGN). The GARP complex is required for the maintenance of the cycling of mannose 6-phosphate receptors between the TGN and endosomes, this cycling is necessary for proper lysosomal sorting of acid hydrolases such as CTSD. Acts as component of the EARP complex that is involved in endocytic recycling. The EARP complex associates with Rab4-positive endosomes and promotes recycling of internalized transferrin receptor (TFRC) to the plasma membrane.[UniProtKB/Swiss-Prot Function]