1810007M14Rik (BC014838) Mouse Untagged Clone

CAT#: MC218379

1810007M14Rik (untagged) - Mouse RIKEN cDNA 1810007M14 gene (cDNA clone MGC:8087 IMAGE:3588322), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: 1810007M14Rik
• Synonyms: 1810007M14Rik; C21orf66; Gcfc; Gcfc1; Pax3/7bp
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >BC014838
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCACCAAATCTTGATTCCTTTGGACGTGATCGGGCACTTTATCAAGAACATGCAAAACGTCGGATTG
  CAGAGCGGGAAGCCAGGAGGACTCGCCGTAGGCAAGCCAGAGAGCAAACCGGTCAGATGGCAGATCACCT
  GGAAGGCCTGTCCAGTGATGATGAAGAGACGTCTACAGATATTACTAATTTCAATCTGGAGAAAGATCGA
  ATTTTGAAAGAGTCTAGCAAAGTTTTTGAAGATGTCCTTGAGAGTTTCTATTCAATTGACTGCATTAAAG
  CACAGTTTGAAGCATGGCGTTCAAAATACTACATGTCCTATAAGGATGCTTATATTGGTCTTTGTCTGCC
  CAAACTGTTCAACCCCCTAATCAGACTTCAGCTTCTCACCTGGACTCCTCTTGAGGCAAAATGCCGTGAC
  TTTGAAACCATGCTGTGGTTTGAATCTCTGCTGTTCTATGGCTGTGAAGACCGAGAGCAGGAGAAGGATG
  AGGCTGACGTTGCTCTGCTGCCCACCATTGTGGAGAAGGTCATTCTCCCCAAGCTGACAGTGATAGCTGA
  AACCATGTGGGATCCCTTTTCTACAACACAGACTTCAAGGATGGTTGGAATCACAATGAAATTAATAAAT
  GGCTACCCCTCAGTAGTCAATGCAGACAATAAAAACACGCAGGTATACCTTAAAGCTCTTTTACTGAGGA
  TGAGAAGAACTTTAGATGATGATGTATTTATGCCCCTATATCCCAAAAATGTCTTAGAAAATAAAAATTC
  TGGGCCTTACTTATTTTTTCAACGACAGTTTTGGTCGTCTGTTAAGCTATTGGGAAATTTTCTTCAGTGG
  TATGGCATTTTCTCAAACAAAACTCTTCAGGAGTTATCAATAGATGGCTTGTTAAACAGATACATCCTTA
  TGGCTTTTCAGAACTCAGAATACGGAGATGACAGCATCAGAAAAGCCCAAAATGTAATCAACTGTTTCCC
  TAAACAGTGGTTTGTGAATCTGAAAGGAGAAAGAACTATTTCTCAGTTAGAAAATTTTTGCCGATATCTT
  GTACATTTAGCAGATACAATTTACAGGAACAGTATTGGGTGCTCTGATGTGGAAAAAAGAAATGCAAGGG
  AAAATATAAAACAGATAGTAAAACTTCTTGCAAGTGTTCGGGCTTTGGATCACGCTATATCTGTTGCAAG
  TGACCACAATGTGAAGGAAGTCAAGTCTCTGATTGAAGGAAAATAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCTGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: BC014838
• Insert Size: 1236 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: BC014838, AAH14838
• RefSeq Size: 1414 bp
• RefSeq ORF: 1235 bp
• Locus ID: 67367
• Cytogenetics: 16 C3.3
• Gene Summary: Adapter protein linking the transcription factors PAX3 and PAX7 to the histone methylation machinery and involved in myogenesis. Associates with a histone methyltransferase complex that specifically mediates dimethylation and trimethylation of 'Lys-4' of histone H3. Mediates the recruitment of that complex to the transcription factors PAX3 and PAX7 on chromatin to regulate the expression of genes involved in muscle progenitor cells proliferation including ID3 and CDC20.[UniProtKB/Swiss-Prot Function]