E2f6 (BC019166) Mouse Untagged Clone

CAT#: MC218297

E2f6 (untagged) - Mouse E2F transcription factor 6 (cDNA clone MGC:29087 IMAGE:4236246), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: E2f6
• Synonyms: EMA, E2F6b
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >BC019166
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCCATCAAAAATAAGGATTAATCTAGAAGAAAATGTACAGTATGTGTCCATGAGAAAAGCTCTGAAAG
  TGAAGAGGCCCCGGTTTGATGTGTCACTGGTATACTTAACTCGGAAGTTTATGGATCTCGTCAGATCTGC
  CCCTGGGGGCATTCTTGACTTAAACAAAGTTGCCACAAAACTGGGTGTTCGGAAGAGGCGAGTGTATGAC
  ATCACCAATGTCTTGGATGGCATCGAACTGGTGGAAAAGAAATCTAAGAACCACATTCGGTGGATAGGAT
  CTGACCTGAACAACTTTGGGGCCGCACCCCAGCAGAAGAAGCTGCAGGCAGAGCTCTCCGACCTGTCGGC
  CATGGAAGACGCCTTGGACGAGTTGATTAAAGATTGTGCTCAGCAACTGTTGGAGTTAACAGATGACAAG
  GAAAATGAAAGACTAGCGTATGTAACCTATCAGGATATTCACGGCATTCAAGCTTTCCATGAACAGATTG
  TCATTGCAGTGAAGGCTCCAGAGGAAACCAGACTGGATGTTCCAGCTCCCAGAGAAGATTCTATCACAGT
  ACATATTAGGAGCACCAAAGGACCCATTGATGTATATTTGTGTGAAGTAGAACAGAACCATTCAAATGGT
  AAAACCAATGATGGAATAGGAGCCTCTCCATCTAAAAGCAGCCATCCACAATGCCCAGAGAAAGAAGACG
  AGCCTCCTCAGTGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCTGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: BC019166
• Insert Size: 714 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: BC019166, AAH19166
• RefSeq Size: 2506 bp
• RefSeq ORF: 713 bp
• Locus ID: 50496
• Cytogenetics: 12 8.04 cM
• Gene Summary: Inhibitor of E2F-dependent transcription. Binds DNA cooperatively with DP proteins through the E2 recognition site, 5'-TTTC[CG]CGC-3'. Has a preference for the 5'-TTTCCCGC-3' E2F recognition site. E2F6 lacks the transcriptional activation and pocket protein binding domains. Appears to regulate a subset of E2F-dependent genes whose products are required for entry into the cell cycle but not for normal cell cycle progression. May silence expression via the recruitment of a chromatin remodeling complex containing histone H3-K9 methyltransferase activity. Overexpression delays the exit of cells from the S-phase (By similarity).[UniProtKB/Swiss-Prot Function]