Rage (BC087925) Mouse Untagged Clone

CAT#: MC218286

Rage (untagged) - Mouse renal tumor antigen (cDNA clone MGC:107277 IMAGE:6532951), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Rage
• Synonyms: MGC46883, MOK, RAGE1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >BC087925
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAAGAGGAGAAGACACCCATTATCAGAGAAAAAGATTATGCTCTACATGTATCAGCTGTGCAAATCGC
  TGGACCACATGCACAGAAACGGGATCTTTCACAGAGACGTGAAGCCGGAGAATATCCTAGTAAAGCAGGA
  TGTCCTGAAACTAGGGGACTTCGGGTCATGCCGGAGTGTCTATTCCAAGCAGCCCTACACAGAGTACATC
  TCTACCCGGTGGTACCGGGCCCCAGAGTGTCTCCTCACCGACGGGTTCTACACATACAAGATGGACCTGT
  GGAGCGCTGGCTGCGTGTTCTACGAGATTGCCAGGGCAGCTGAGACACAGACCCTGGCCAAACACAGAAG
  AGCTTTCTGTCCCAAGTTCTCCATGGTGCCAGAATCATCCAGTCACAACTGGTCATTCTCACAGGAAGGC
  AGAAAGCAGAAACAGTCCCTGCGGCACGAGGAGGGCCATGCCAGACGACAGGGGCCGACCAGCTTGATGG
  AGCTTCCCAAACTGAGGCTGTCGGGAATGACCAAACTGTCATCATGTTCCAGCCCTGCACTGCGGTCAGT
  GCTGGGCACTGGAGCAAATGGAAAAGTCCCTGTGCTGAGACCCCTGAAGTGTGCTGCTGTGAACAAGAAG
  ACAGACACGCAGAAGGACATCAAACCTCACCTGAAACACTATCACCTGCCCACGATCAACAGGAAAGGGG
  GCGAATACTGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCTGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: BC087925
• Insert Size: 711 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: BC087925, AAH87925
• RefSeq Size: 1161 bp
• RefSeq ORF: 710 bp
• Locus ID: 26448
• Cytogenetics: 12 F1
• Gene Summary: Able to phosphorylate several exogenous substrates and to undergo autophosphorylation (PubMed:10421840). Negatively regulates cilium length in a cAMP and mTORC1 signaling-dependent manner (PubMed:25243405).[UniProtKB/Swiss-Prot Function]