Srp54 (BC005543) Mouse Untagged Clone

CAT#: MC218281

Srp54 (untagged) - Mouse signal recognition particle 54 (cDNA clone MGC:6231 IMAGE:3585216), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Srp54
• Synonyms: 54kDa
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >BC005543
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGTTCTAGCAGATCTTGGAAGAAAAATAACATCAGCATTACGCTCATTGAGCAATGCCACCATTATCA
  ATGAAGAGGTATTAAATGCTATGCTGAAAGAAGTATGTACAGCATTATTGGAAGCAGATGTTAATATTAA
  ACTAGTGAAGCAACTCAGAGAAAATGTTAAGTCTGCAATTGATCTTGAAGAGATGGCATCTGGACTGAAC
  AAAAGAAAAATGATTCAGCATGCTGTATTTAAAGAACTTGTAAAGCTTGTAGACCCTGGAGTTAAAGCGT
  GGACACCGACTAAGGGAAAGCAAAATGTGATCATGTTTGTTGGATTGCAAGGGAGTGGTAAAACGACAAC
  ATGTTCAAAGTTAGCATATTATTACCAGAGGAAAGGTTGGAAGACCTGTTTGATATGTGCAGATACATTC
  AGAGCAGGAGCCTTTGACCAGCTAAAACAGAATGCCACCAAAGCAAGAATTCCGTTCTATGGCAGCTATA
  CTGAAATGGACCCTGTCATCATTGCTTCTGAAGGAGTGGAGAAATTCAAAAATGAAAATTTTGAAATTAT
  TATTGTTGATACAAGTGGTCGTCACAAACAAGAAGACTCTTTATTTGAAGAAATGCTTCAAGTTTCTAAT
  GCTATACAACCTGATAACATTGTTTATGTGATGGATGCATCCATCGGACAGGCTTGTGAGGCCCAGGCGA
  AGGCCTTTAAAGACAAAGTAGATGTAGCTTCAGTAATAGTGACAAAACTCGACGGTCACGCGAAAGGAGG
  CGGTGCTCTTAGTGCAGTTGCTGCCACAAAAAGTCCAATTATTTTCATTGGTACAGGGGAACATATAGAT
  AATTTTGAACCTTTCAAAACACAACCTTTCATCAGCAAACTCCTTGGAATGGGTGATATTGAAGGACTGA
  TTGATAAAGTCAATGAATTGAAGTTGGATGATAACAAGGCACTTATAGAGAAGTGGAAGCACGGTCAGTT
  TACATTGCGAGACATGTATGAACAGTTTCAGAATATTATGAAAATGGGCCCATTCAGTCAGATATTGGGG
  ATGATTCCTGGCTTTGGCACAGATTTTATGAGCAAAGGGAATGAGCAGGAGTCAATGGCAAGGCTGAAGA
  AACTGATGACAATCATGGACAGTATGAACGATCAAGAACTGGACAGTACAGATGGTGCCAAGGTTTTCAG
  TAAGCATCCAGGGAGAATCCAAAGAGTTGCCCGGGGATCAGGTGTGTCAACAAGAGATATTCAAGAACTT
  CTGACCCAGTATACCAAGTTTGCACAGATGGTCAAAAAGATGGGAGGTATCAAAGGACTTTTCAAAGGCG
  GTGATATGTCTAAGAATGTGAGTCAGTCACAGATGGCAAAATTAAACCAACAAATGGCCAAAATGATGGA
  CCCACGAGTTCTTCATCACATGGTGTGTGACTTTCTTGGAGAGAAGATTGCGTCAGTTTTGGGCATCAGC
  ACCCCAAAATACCAGTACGCCATTGATGAGTAA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCTGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: BC005543
• Insert Size: 1503 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: BC005543, AAH05543
• RefSeq Size: 1986 bp
• RefSeq ORF: 1502 bp
• Locus ID: 24067
• Cytogenetics: 12 C1