Oas1b (BC012877) Mouse Untagged Clone

CAT#: MC218278

Oas1b (untagged) - Mouse 2'-5' oligoadenylate synthetase 1B (cDNA clone MGC:19005 IMAGE:4016177), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Oas1b
• Synonyms: Flv; L1; Mmu-L1; Oas1; Oias-2; Oias2; Wnv
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >BC012877
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAGCAGGATCTGAGGAGCATCCCGGCCTCGAAGCTTGATAAGTTCATAGAGAATCATCTCCCGGACA
  CCAGCTTCTGTGCTGACCTCAGAGAAGTCATAGATGCCCTGTGTGCTCTCCTGAAGGACAGATTCTTCCG
  GGGCCCCGTCCGCCGAATGAGGGCCTCTAAGGGGGTCAAGGGCAAATGCACCGCGCTCAAGGGCAGGTCA
  GACGCTGACCTGGTGGTGTTCCTTAACAATCTCACCTACTTTGAGGATCAATTAAACCAACAGGGAGTGT
  TGATTAAGGAAATTAAGAAACAGCTGTACGAGGTTCAGCATGAGAGACGTTTTGGAGTCAAGTTTGAGGT
  CCAGAGTTTAAGGAGTCCCAACTCCCGGGCTCTGAGCTTCAAGCTGAGCGCCCCCGACCTGCTGAAGGAG
  GTGAAGTTTGACGTGCTGCCAGCCTATGATTTACTGGATCATCTTAACATCCTCAAGAAGCCTAACCAAC
  AATTCTACGCCAATCTCATCAGTGGGCGTACCCCGCTGGGGAAGGAGGGCAAGTTATTGACCTGCTTTAT
  GGGGCTTCGGAAGTACTTCCTGAACTGTCGCCCAACCAAGCTGAAGCGCCTCATCCACCTGGTCACGCAC
  TGGTACCAACTGTGTAAGGAGAAGCTGGGGGACCCGCTGCCCCCACAGTATGCCCTGGAGCTGCTCACAG
  TCTATGCCTGGGAGTATGGGAGTCGAGTAACTAAATTCAACACAGCCCAGGGCTTCCGAACCGTCTTGGA
  ACTGGTTACCAAGTACAAACAGCTTCGAATCTACTGGACAGTGTATTATGACTTTCGACATCAAGAGGTC
  TCTGAATACCTGCACCAACAGCTCAAAAAAGACAGGCCTGTGATCTTGGACCCCGCTGATCCAACAAGGA
  ATATAGCTGGTTTGAACCCAAAGGACTGGCGGCGTCTAGCAGGAGAGGCTGCCGCCTGGCTGCAATACCC
  ATGCTTTAAGTACAGGGACGGTTCCCCAGTGTGCTCCTGGGAGGTGCCGACGGAGGTTGCAGTGCCAACG
  AAGTATCTCTTTTGTCGTATTTTCTGGTTATTGTTTTGGTCTTTGTTTCATTTCATCTTTGGGAAGACTT
  CATCTGGATAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCTGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: BC012877
• Insert Size: 1131 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: BC012877, AAH12877
• RefSeq Size: 1944 bp
• RefSeq ORF: 1130 bp
• Locus ID: 23961
• Cytogenetics: 5 60.64 cM
• Gene Summary: This gene is a member of the 2'-5' oligoA synthetase family, which are clustered on chromosome 5. The encoded protein functions in the interferon-regulated OAS/RNase L system, which mediates RNA decay as part of the innate antiviral immunity pathway. The protein binds double-stranded RNA and oligomerizes ATP, which activate the single-stranded RNA cleavage enzyme RNase L. This protein mediates resistance to flaviviruses such as West Nile virus. The majority of wild mouse strains produce a functional protein and are resistant to flavivirus infection, whereas some inbred mouse strains including the strain of the reference genome, C57BL/6J, contain a premature stop codon that inactivates this gene product. [provided by RefSeq, Jul 2008]