Fv1 (BC052897) Mouse Untagged Clone

CAT#: MC218168

Fv1 (untagged) - Mouse Friend virus susceptibility 1 (cDNA clone MGC:60603 IMAGE:30060270), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Fv1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >BC052897
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAATTTCCCACGTGCGCTTGCTGGTTTCTCGAGCTGGCTCTTCAAACCTGAACTTGCCGAGGACTCTC
  CGGATAATGACTCTCCGGATAATGACACTGTTAACCCATGGCGTGAGCTGCTGCAGAAGATAAATGTGGC
  CGATCTCCCCGATTCATCCTTTTCGAGCGGTAAGGAACTTAATGACTCTGTGTACCATACTTTTGAACAT
  TTCTGCAAGATTAGGGACTATGACGCAGTTGGCGAGCTGCTTCTGGCATTTCTGGATAAAGTAACAAAGG
  AAAGGGACCAATTCAGAGATGAAATTTCCCAGCTCCGAATGCACATAAATGATCTAAAGGCTTCTAAGTG
  TGTCCTGGGGGAGACTCTTCTTTCCTACCGCCACAGGATTGAAGTTGGGGAAAAACAGACTGAAGCCCTC
  ATTGTGAGGTTAGCTGATGTGCAATCCCAGGTCATGTGTCAGCCTGCCCGGAAAGTGTCTGCAGATAAGG
  TGAGGGCACTGATTGGTAAAGAATGGGATCCGGTAACCTGGGATGGAGATGTGTGGGAGGACATAGATTC
  TGAAGGGTCTGAGGAAGCTGAGTTGCCCACTGTCTTGGCCTCTCCATCCTTGTCTGAGGAAAGTGGTTAT
  GCCTTGTCTAAAGAACGCACCCAGCAGGACAAAGCAGATGCCCCTCAGATCCAGTCTTCAACATCCTTAG
  TTACTTCTGAACCTGTCACCAGACCCAAGTCTCTGTCTGACCTTACAAGTCAGAAACACCGCCATACTAA
  TCATGAACTCAATTCACTTGCTCACTCAAATCGCCAAAAGGCAAAGGAACATGCTAGGAAATGGATTTTA
  AGGGTGTGGGATAATGGTGGGAGGCTCACAATACTGGATCAGATTGAATTTCTCAGTTTAGGTCCTTTGA
  GCCTTGATAGTGAGTTTAATGTCATAGCCCGCACTGTTGAAGATAATGGTGTGAAGAGTTTGTTTGATTG
  GTTGGCTGAAGCATGGGTCCAGAGATGGCCTACTACAAGAGAGCTGCAGTCGCCTGACACCCTGGAGTGG
  TATTCTATTGAGGATGGGATTAAAAGGCTTAGGGAACTTGGAATGATAGAGTGGCTTTGTGTAAAAGCTA
  CTTGTCCACAGTGGAGGGGCCCGGAAGATGTACCCATCACGAGAGCTATGAGGATAACTTTTGTCCGGGA
  AACTGTAGAGACTTGGAAGAGCTTTGTATTTAGCCTCCTCTGTATAAAGGACATAACAGTGGGGAGCGTG
  GCTGCTCAGTTGCATGATCTAATAGAATTAAGTTTAAAGCCAACAGCAGCTACAAAACTCTGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCTGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: BC052897
• Insert Size: 1500 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: BC052897
• RefSeq Size: 2654 bp
• RefSeq ORF: 1322 bp
• Locus ID: 14349
• Cytogenetics: 4 78.56 cM
• Gene Summary: Retroviral restriction factor that prevents infection by gammaretroviruses. Acts by interacting with the capsid protein ca after entry of the virus into the cell. This interaction presumably disrupt the capsid thereby inactivating the viral genome, making it unable to enter host nucleus and integrate into host genome.[UniProtKB/Swiss-Prot Function]