B3gat1 (BC023052) Mouse Untagged Clone

CAT#: MC217917

B3gat1 (untagged) - Mouse beta-1,3-glucuronyltransferase 1 (glucuronosyltransferase P) (cDNA clone MGC:38784, (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: B3gat1
• Synonyms: GlcAT-P, HNK-1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >BC023052
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTCCGACCGTGACATCGTGGAAGTGGTGCGCACAGAGTACGTGTACACGAGGCCGCCACCCTGGTCCG
  ACACGCTGCCCACCATCCATGTGGTGACGCCCACCTACAGTAGACCGGTGCAGAAGGCAGAGCTGACGCG
  AATGGCCAACACACTACTGCATGTGCCCAACCTTCACTGGCTAGTGGTGGAGGATGCTCCACGCAGGACG
  CCCCTCACCGCGCGCTTGCTGCGCGACACTGGCCTCAACTACACACACCTGCACGTAGAGACACCACGCA
  ACTATAAGCTGCGAGGTGATGCCCGAGACCCTCGCATCCCACGTGGCACCATGCAGCGCAACCTGGCACT
  GCGCTGGCTGCGGGAGACCTTCCCACGGAACTCTACTCAGCCAGGCGTAGTGTACTTCGCGGATGATGAC
  AACACGTACAGTCTGGAGCTCTTTGAAGAGATGCGCAGCACAAGAAGGGTGTCCGTGTGGCCTGTGGCCT
  TTGTTGGTGGCCTTCGGTATGAGGCCCCACGGGTGAATGGGGCAGGGAAAGTGGTTGGCTGGAAGACAGT
  CTTCGACCCCCACCGGCCCTTTGCAATAGACATGGCTGGATTTGCTGTCAACCTCCGGCTCATCTTGCAG
  CGAAGTCAAGCCTACTTTAAGCTACGTGGTGTGAAGGGAGGCTACCAGGAAAGCAGTCTCCTTCGAGAAC
  TTGTCACCCTCAATGACCTGGAGCCCAAGGCAGCAAACTGTACCAAGATCTTGGTCTGGCATACACGAAC
  AGAGAAGCCAGTGCTGGTCAATGAGGGGAAGAAGGGCTTCACCGACCCCTCAGTGGAGATCTGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCTGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: BC023052
• Insert Size: 834 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: BC023052, AAH23052
• RefSeq Size: 1709 bp
• RefSeq ORF: 833 bp
• Locus ID: 76898
• Cytogenetics: 9 A4
• Gene Summary: Involved in the biosynthesis of L2/HNK-1 carbohydrate epitope on glycoproteins. Can also play a role in glycosaminoglycan biosynthesis. Substrates include asialo-orosomucoid (ASOR), asialo-fetuin, and asialo-neural cell adhesion molecule. Requires sphingomyelin for activity: stearoyl-sphingomyelin was the most effective, followed by palmitoyl-sphingomyelin and lignoceroyl-sphingomyelin. Activity was demonstrated only for sphingomyelin with a saturated fatty acid and not for that with an unsaturated fatty acid, regardless of the length of the acyl group.[UniProtKB/Swiss-Prot Function]