Acss1 (BC030930) Mouse Untagged Clone

CAT#: MC217858

Acss1 (untagged) - Mouse acyl-CoA synthetase short-chain family member 1 (cDNA clone MGC:31687 IMAGE:4911679), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Acss1
• Synonyms: 1110032O15Rik; Acas2; Acas2l; AceCS2; AI788978
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >BC030930
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCCAAGGAGGCCCCTGTTTGCACCCCTGAGAGCATGAGCAGTGAAGACATGCTCTTTATGCTCTACA
  CCTCAGGGAGCACCGGGACACCCAAGGGACTCGTTCATACACAGGCAGGCTATCTACTGTATGCCGCCAT
  GACGCACAAGCTCGTGTTTGACTACCAGCCAGGTGATGTCTTTGGCTGTGTGGCTGACATCGGTTGTATC
  ACAGGACACAGCTATGTGGTGTATGGACCCCTCTGCAATGGAGCTACCACAGTCCTTTTTGAGAGCACCC
  CAGTTTACCCTGATGCTGGTCGTTACTGGGAGACAGTGCAGAGGCTAAAGATCAACCAGTTCTATGGTGC
  CCCGACAGCTGTCCGGCTGCTGCTGAAGTATGGGGATGCCTGGGTGAAAAAGTATGACCGCTCTTCCCTG
  CGCACACTGGGGTCAGTGGGAGAGCCTATCAACCACGAAGCCTGGGAGTGGCTCCACAAAGTCGTGGGTG
  ATGGCAGATGTACACTGGTGGACACTTGGTGGCAAACGGAAACTGGAGGCATCTGCATTGCACCACGGCC
  CTCGGAAGATGGGGCAGAGATCCTCCCGGGCATGGCCATGAGGCCGTTTTTTGGCATCGTTCCTGTACTC
  ATGGATGAGAAGGGCAATGTTTTGGAGGGTGGAGATGTCTCTGGGGCCTTGTGTATTTCCCAAGCTTGGC
  CAGGCATGGCAAGGACCATCTATGGTGACCACCAGAGGTTCGTAGATGCCTACTTCAGAGCGTACCCAGG
  TTATTACTTCACTGGAGACGGAGCTCACCGGACAGAGGGTGGCTATTACCAGATCACGGGGCGCATGGAT
  GATGTCATCAATATCAGTGGTCATCGCCTGGGGACTGCAGAGATTGAGGATGCAATGGCTGACCATCCCG
  CTGTTCCAGAGACTGCTGTCATTGGGTACCCTCATGATATCAAAGGAGAAGCTGCATTTGCCTTCATTGT
  GCTGAAAGATAACATCAGTGATGAGAACATGGTAGTGAATGAACTCAAATTGTCGGTGGCCACCAAGATC
  GCCAAGTATGCTGTGCCTGACCAGATCCTGGTGGTGAAGCGTCTCCCCAAAACCAGATCTGGGAAAGTGA
  TGAGGAGACTACTGAGGAAGATCATCACCAGCAGGGGACAGGATCTAGGGGACACCACTACCCTGGAGGA
  CCCCAGCGTCATCACAGAAATCTTGAGTGCCTTCCAGAAGTATGAAGAGCAGCGGGCTGCTACCAACTGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCTGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: BC030930
• Insert Size: 1260 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: BC030930, AAH30930
• RefSeq Size: 2783 bp
• RefSeq ORF: 1259 bp
• Locus ID: 68738
• Cytogenetics: 2 G3
• Gene Summary: Important for maintaining normal body temperature during fasting and for energy homeostasis. Essential for energy expenditure under ketogenic conditions. Converts acetate to acetyl-CoA so that it can be used for oxidation through the tricarboxylic cycle to produce ATP and CO(2).[UniProtKB/Swiss-Prot Function]