Miga1 (NM_001162375) Mouse Untagged Clone

CAT#: MC217641

Fam73a (untagged) - Mouse family with sequence similarity 73, member A (Fam73a), transcript variant 2, (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Miga1
• Synonyms: A930038D06; AI852168; C030011O14Rik; Fam73a; Mita1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC217641 representing NM_001162375
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTCAGACGAGACAGTCAGCAGATCACAGTTCTCCTTGAAGACGTACGCAGTTAGAGTGTTTGCTCTCC
  CTGTGTCTTGGTATTATTCTCTCTCCCAGATCAAATTTTCACCAGTAGCTAAGAAGCTGTTCATGGTAAC
  TGCCGTGAGTGCTGTGTCTGTGATTTTTCTGGCCCATCACTTTAAAAGAAGACGGGGAAAACAGAAGGGC
  AAAGTGCTGCCCTGGGAACCCGAGCACCTCCTCCTTGAGCACACTAGGAGAGCTGCATCAGAAAAAGGTT
  CGAGTTGCTCCAGCAGTCGGCAGAATCTGACCTTGTCGTTAAGCTCCACCAAGGAGAAAGGATCTCAGTG
  TTGCAACTACCCGAATGGAGGGCTTCTCAGCAGATACTCGGGCTCTGCCCAGAGCCTGGGCTCCGTCCAG
  AGTGTTAACTCCTGTCATAGTTGTGCTTGTGGAAATTCTAATTCCTGGGACAAAGCAGACGATGATGACA
  TCAGACTTGTTAACATTCCTGTGACCACACCTGAGAACTTATACCTGATGGGTATGGAATTATTTGAAGA
  GGCGTTACGACGATGGGAACAAGCTCTGACTTTTCGAAGTAGACAGGCTGAAGATGAAGCCTGCAGCTCT
  GTTAAACTGGGAGCCGGAGATGCCATTGCAGAAGAAAGTGTGGATGATATTATTAGTTCAGAATTCATCC
  ATAAACTTGAAGCTCTACTGCAAAGAGCGTATCGTCTCCAAGAGGAGTTTGAGGCTACCCTTGGGGGATC
  TGACCCTAATTCCATTGCAAATGATACTGATAAAGACACAGACATGAGCTTGAGGGAGACTATGGATGAG
  CTTGGCCTGCCAGACGCCATGAACATGGACTCAGCAGATCTCTTTGCTTCAGCAACAGAGCTAATCCTTG
  CAGAAGCAGACAACAGGTCATTCCTTGCTGAAAGTGGAAGGAAAATTTTGTCAGCTTTAATTGTGAAAGC
  ACGGAAGAATCCAAAGAAATTTCAAGATGTTTTTGATGAAATGATCAATTTTTTAGAGCAGACTGATCAC
  TGGGATAGCACTGAGCTGGAACTTGCTGCCAGAGGGGTGAAAAATTTAAATTTTTATGACGTGGTTCTGG
  ATTTTATATTAATGGATTCCTTTGAAGATTTAGAAAACCCACCGACATCCATACAGAGCGTAGTGAACAA
  TCGCTGGCTCAACTCTTCCTTCAAAGAAAGCGCTGTGGCTTCAAGTTGTTGGTCAGTGCTGAAACAGAAA
  AGGCAACAGATGAAGATCTCAGATGGATTTTTCGCTCATTTTTATGCCATTTGTGAGCATGTCAGCCCTG
  TTTTAGCCTGGGGCTTCTTGGGTCCTAGAAATTCTCTCTATGACTTATGTTGCTTCTTTAAGAATCAAGT
  TCTTTTCTTTCTCAAAGACATCTTCGACTTTGAGAAGGTGCGCTATTCGAGCATAGACACGCTGGCGGAG
  GACCTCACGCACCTACTCATCCGCCGCACGGAGCTGCTGGTCACCTGTCTTGGTGCAGATGCCCTGAGGC
  ACGCCACCACTTGCACGAGTGGACACTCCCACGCCGTGCCCACTGCGCTCTTAGAAGCCAAAGTACAATA
  A
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001162375
• Insert Size: 1611 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001162375.1, NP_001155847.1
• RefSeq Size: 5163 bp
• RefSeq ORF: 1611 bp
• Locus ID: 215708
• UniProt ID: Q4QQM5
• Cytogenetics: 3 H3
• Gene Summary: Regulator of mitochondrial fusion (PubMed:26711011). Acts by forming homo- and heterodimers at the mitochondrial outer membrane and facilitating the formation of PLD6/MitoPLD dimers. May act by regulating phospholipid metabolism via PLD6/MitoPLD (By similarity).[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (2) lacks two exons in the coding region compared to variant 1. The encoded isoform (2) is shorter but has the same N- and C-termini compared to isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.