Bco2 (NM_133217) Mouse Untagged Clone

CAT#: MC217633

Bco2 (untagged) - Mouse beta-carotene oxygenase 2 (Bco2), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Bco2
• Synonyms: B-diox-II; Bcdo2; Bcmo2; CMO2
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC217633 representing NM_133217
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTTGGGACCGAAGCAGAGCCTGCCATGCATTGCCCCACTGCTGACCACGGCGGAGGAGACTCTGAGTG
  CTGTCTCTGCTCGGGTCCGAGGACATATTCCTGAATGGCTTAATGGTTATCTACTTCGAGTTGGACCTGG
  GAAGTTTGAATTTGGGAAGGATAGATACAATCATTGGTTTGATGGAATGGCGTTGCTTCACCAGTTCCGA
  ATGGAGAGGGGCACAGTGACATACAAGAGCAAGTTTCTACAGAGTGACACATATAAGGCCAACAGTGCTG
  GAGGTAGAATTGTGATCTCAGAATTTGGCACGCTGGCCCTTCCTGACCCATGCAAGAGCATCTTTGAACG
  TTTCATGTCAAGGTTTGAGCCACCTACTATGACTGACAACACCAACGTCAACTTTGTGCAGTACAAAGGT
  GATTACTACATGAGCACAGAGACTAATTTTATGAATAAGGTGGACATTGAGATGCTGGAAAGGACAGAAA
  AGGTGGACTGGAGCAAATTCATTGCTGTGAATGGAGCCACTGCACATCCTCATTACGACCCAGATGGGAC
  AGCATACAACATGGGGAACAGCTATGGGCCAAGAGGTTCTTGCTATAATATTATTCGTGTTCCTCCAAAA
  AAGAAAGAGCCCGGGGAGACGATTCACGGAGCACAGGTGCTATGTTCCATTGCCTCCACTGAGAAAATGA
  AGCCTTCTTACTACCATAGCTTTGGAATGACAAAAAACTACATAATCTTTGTCGAACAGCCTGTAAAGAT
  GAAGCTGTGGAAAATAATCACTTCTAAAATCCGGGGAAAGCCCTTTGCTGATGGGATAAGCTGGGAGCCC
  CAGTATAACACGCGGTTTCATGTGGTGGATAAACACACTGGACAGCTTCTCCCAGGAATGTACTACAGCA
  TGCCTTTTCTTACCTATCATCAAATCAATGCCTTTGAGGACCAGGGCTGTATTGTGATTGATCTGTGCTG
  CCAGGATGATGGGAGAAGCCTAGACCTTTACCAACTACAGAATCTCAGGAAAGCTGGAGAGGGGCTTGAT
  CAGGTCTATGAGTTAAAGGCAAAGTCTTTCCCTCGAAGATTTGTCTTGCCCTTAGATGTTAGTGTGGATG
  CTGCTGAAGGAAAGAACCTCAGCCCACTGTCCTATTCTTCAGCCAGCGCTGTGAAACAGGGTGATGGAGA
  GATCTGGTGCTCTCCTGAAAATCTACACCACGAAGACCTGGAAGAGGAAGGGGGGATTGAATTCCCTCAG
  ATCAACTATGGCCGATTCAATGGCAAAAAGTATAGTTTCTTCTATGGCTGCGGTTTTCGACATTTGGTGG
  GGGATTCTCTGATTAAGGTTGACGTGACGAACAAGACACTAAGGGTTTGGAGAGAAGAAGGCTTTTATCC
  CTCGGAGCCCGTTTTTGTTCCGGTGCCAGGAGCAGATGAGGAAGACAGTGGGGTTATACTCTCTGTGGTG
  ATCACTCCCAACCAGAGTGAAAGCAACTTCCTCCTTGTCTTGGATGCCAAGAGCTTCACAGAGCTGGGGC
  GAGCGGAAGTACCCGTGCAGATGCCTTACGGGTTCCATGGCACCTTTGTGCCTATCTGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_133217
• Insert Size: 1599 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_133217.3, NP_573480.1
• RefSeq Size: 2093 bp
• RefSeq ORF: 1599 bp
• Locus ID: 170752
• UniProt ID: Q99NF1
• Cytogenetics: 9 A5.3
• Gene Summary: Asymmetrically cleaves beta-carotene at the 9',10' double bond resulting in the formation of beta-apo-10'-carotenal and beta-ionone. Besides beta-carotene, lycopene is also oxidatively cleaved. The apocarotenals formed by this enzyme may be the precursors for the biosynthesis of retinoic acid or exert unknown physiological effects.[UniProtKB/Swiss-Prot Function]