Bbof1 (NM_028377) Mouse Untagged Clone

CAT#: MC217615

Ccdc176 (untagged) - Mouse RIKEN cDNA 2900006K08 gene (2900006K08Rik), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Bbof1
• Synonyms: 2900006K08Rik; AI427784; Ccdc176
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC217615 representing NM_028377
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCCGGCGAAGGACAAGAGGAAAGACAAGAGGAAAGACAAGAGGAAAGGCAAAAACAAAGGCAAAGAAC
  CAAAGAAAATAATCAAATCAGATGAACCTGCAATTGAAAGAGCCAAAGCCAATGCCTCTCTTTGGGAGGC
  CAGATTGGAAGTCACAGAACTCTCTAGGATTGAATATCGTGATACTTCCCGAAGACTGGCAAAAAATAAT
  GAAGACTTAAAGAAACAGCAATATAACATGGAAAAAGACATTATGTCTGTATTAAGCTACTTGAAGAAGC
  AGGATCAAGAGAAAGATAATATGATAGAAAAACTGAAGCAGCAGTTGGCTAAAACAAAGGAAAAAGCCCA
  GGAAGAGAAGGAAAAATTGGAACAAAAGTATGCTCTGCAAGTGAGTGAGTTGGAGGGACAGTTTCATCAA
  AAAGCCAAGGAAATCGGCATGATTCAGACAGAGCTAAAGACAATAAAACAATTCCAGAAGAGAAAAATAC
  AAGTGGAGAAAGAATTAGATGATCTGAAAGAAAATTTAAGGAACTCAGAGAAGAACTATCAGGAGACTCT
  TAGAAGACTAGAAAGCAGATTTTTTGAAGAAAAGCACCGTTTGGAGCAAGAGGCTGAGAAGAGGATCATA
  ATGCTGGCAGAGAGAGCTCACCATGAAGCTGTAGTGCAGTTGAACACTGCTGGAAGAAATGTTTTCAAAG
  AGAATGTTTATCTTCACAAGGCGCTTGCATACCACTTGAAGGAAGCTGAAATTCTCCAGCAAAACTCCAA
  GAAGATTGAAGAAAACCATTCCTGCCTTTTACAACAAAAGGAAATCAATGATCTGTTGGTTAAGGAAAAG
  ATTATGCAACTCACCCAGCAGAAATCACAAATCCAAACTCTCCAGAAGAAGGTGGTAAGCTTGGAGAACG
  CTCTGACCTACATGACCACAGAGTTTGAGGCTGAAGTGTTGAAACTGCAGCAAAAGGCAATCATAGAGAA
  CCAAGCAGGCTTGGTAGAGATTGACAAGCTGCAGCAACTTCTTCAGATGAAGGACAGGGAGATGAACCGA
  GTGAAGAGGCTGGCTAAGAACATACTGGATGAGAGGACAGAAGTGGAACAGTTCTTTCTAGACGCTCTCT
  ACGAAGTGAAGCAACAGATTCTGGCCAGCAGGAAGCATTATAAGCAGATAGCACAAGCTGCTTTCAATTT
  AAAAATGAGAGCAGCATGTGCGGGAAAAACAGAATATCCCAGAATCAGGACCTTTGATGGCAAAGAACAA
  AGTACCAATAGTGTGAACCAGGATCTCTTGGAGGCTGAAAAATGGCCAACTACTCAAAAAAATGTGGATA
  TTAGAGATTTGACCTGGGAGCAGAAGGAAAAGGTCTTAAGATTGCTCTTTGCAAAAATGAATGGTTTTGC
  TGCTAGGAAATACAGTCAGAGTTCTAAGCCTCCTGTTCCAGACCATATTATTTATGGCAGTGGAGAAATG
  AAAGAAACTGGGGATGAAAATAACCTTCTAGATCAAACCTTCATCACCCAGCAAGCGCCAGTATCAGACT
  CCAATAGAATGGTGTCACCCGATGTTATTCCTCAAGGATTACAGGACTCTGACATAGCCTAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_028377
• Insert Size: 1602 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_028377.3, NP_082653.2
• RefSeq Size: 2190 bp
• RefSeq ORF: 1602 bp
• Locus ID: 72873
• UniProt ID: Q3V079
• Cytogenetics: 12 D1
• Gene Summary: Basal body protein required in multiciliate cells to align and maintain cilia orientation in response to flow. May act by mediating a maturation step that stabilizes and aligns cilia orientation. Not required to respond to planar cell polarity (PCP) or flow-based orientation cues (By similarity).[UniProtKB/Swiss-Prot Function]