Tyr (NM_011661) Mouse Untagged Clone

CAT#: MC217582

Tyr (untagged) - Mouse tyrosinase (Tyr), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Tyr
• Synonyms: albino; c; Oca1; skc35
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC217582 representing NM_011661
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTTCTTGGCTGTTTTGTATTGCCTTCTGTGGAGTTTCCAGATCTCTGATGGCCATTTTCCTCGAGCCT
  GTGCCTCCTCTAAGAACTTGTTGGCAAAAGAATGCTGCCCACCATGGATGGGTGATGGGAGTCCCTGCGG
  CCAGCTTTCAGGCAGAGGTTCCTGCCAGGATATCCTTCTGTCCAGTGCACCATCTGGACCTCAGTTCCCC
  TTCAAAGGGGTGGATGACCGTGAGTCCTGGCCCTCTGTGTTTTATAATAGGACCTGCCAGTGCTCAGGCA
  ACTTCATGGGTTTCAACTGCGGAAACTGTAAGTTTGGATTTGGGGGCCCAAATTGTACAGAGAAGCGAGT
  CTTGATTAGAAGAAACATTTTTGATTTGAGTGTCTCCGAAAAGAATAAGTTCTTTTCTTACCTCACTTTA
  GCAAAACATACTATCAGCTCAGTCTATGTCATCCCCACAGGCACCTATGGCCAAATGAACAATGGGTCAA
  CACCCATGTTTAATGATATCAACATCTACGACCTCTTTGTATGGATGCATTACTATGTGTCAAGGGACAC
  ACTGCTTGGGGGCTCTGAAATATGGAGGGACATTGATTTTGCCCATGAAGCACCAGGGTTTCTGCCTTGG
  CACAGACTTTTCTTGTTATTGTGGGAACAAGAAATTCGAGAACTAACTGGGGATGAGAACTTCACTGTTC
  CATACTGGGATTGGAGAGATGCAGAAAACTGTGACATTTGCACAGATGAGTACTTGGGAGGTCGTCACCC
  TGAAAATCCTAACTTACTCAGCCCAGCATCCTTCTTCTCCTCCTGGCAGATCATTTGTAGCAGATCAGAA
  GAGTATAATAGCCATCAGGTTTTATGCGATGGAACACCTGAGGGACCACTATTACGTAATCCTGGAAACC
  ATGACAAAGCCAAAACCCCCAGGCTCCCATCTTCAGCAGATGTGGAATTTTGTCTGAGTTTGACCCAGTA
  TGAATCTGGATCAATGGATAGAACTGCCAATTTCAGCTTTAGAAACACACTGGAAGGATTTGCCAGTCCA
  CTCACAGGGATAGCAGATCCTTCTCAAAGTAGCATGCACAATGCCTTACATATCTTTATGAATGGAACAA
  TGTCCCAAGTACAGGGATCGGCCAACGATCCCATTTTTCTTCTTCACCATGCTTTTGTGGACAGTATTTT
  TGAACAATGGCTGCGAAGGCACCGCCCTCTTTTGGAAGTTTACCCAGAAGCCAATGCACCTATCGGCCAT
  AACAGAGACTCTTACATGGTTCCTTTCATACCGCTCTATAGAAATGGTGATTTCTTCATAACATCCAAGG
  ATCTGGGATATGACTACAGCTACCTCCAAGAGTCAGATCCAGGCTTTTACAGAAATTATATTGAGCCTTA
  CTTGGAACAAGCCAGTCGTATCTGGCCATGGCTTCTTGGGGCAGCACTGGTGGGAGCTGTTATTGCTGCA
  GCTCTCTCTGGGCTTAGCAGTAGGCTATGCCTTCAGAAGAAGAAGAAGAAGAAGCAACCCCAGGAGGAAA
  GGCAGCCACTCCTCATGGACAAAGACGACTACCACAGCTTGCTGTATCAGAGCCATCTGTGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_011661
• Insert Size: 1602 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_011661.5, NP_035791.1
• RefSeq Size: 6042 bp
• RefSeq ORF: 1602 bp
• Locus ID: 22173
• UniProt ID: P11344
• Cytogenetics: 7 49.01 cM
• Gene Summary: This is a copper-containing oxidase that functions in the formation of pigments such as melanins and other polyphenolic compounds. Catalyzes the initial and rate limiting step in the cascade of reactions leading to melanin production from tyrosine. In addition to hydroxylating tyrosine to DOPA (3,4-dihydroxyphenylalanine), also catalyzes the oxidation of DOPA to DOPA-quinone, and possibly the oxidation of DHI (5,6-dihydroxyindole) to indole-5,6 quinone (PubMed:2494997, PubMed:2517217, PubMed:1537333).[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.