P4ha1 (NM_011030) Mouse Untagged Clone

CAT#: MC217546

P4ha1 (untagged) - Mouse procollagen-proline, 2-oxoglutarate 4-dioxygenase (proline 4-hydroxylase), alpha 1 polypeptide (P4ha1), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: P4ha1
• Synonyms: AL022634; P4ha
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC217546 representing NM_011030
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGATCTGGGTTGTTTTAATGATGGCGATTCTACTTCCTCAGTCTTTAGCCCATCCAGGGTTTTTTACGT
  CAATTGGTCAGATGACTGACTTGATCCATAATGAGAAAGACCTGGTGACATCACTAAAAGATTATATTAA
  AGCAGAAGAGGACAAGTTAGAGCAAATCAAAAAATGGGCAGAGAAGTTAGACCGGCTAACGAGTACAGCA
  ACAAAAGACCCAGAGGGGTTTGTTGGGCATCCTGTAAACGCATTCAAGTTAATGAAACGTCTGAACACTG
  AGTGGAGTGAGTTGGAGAATCTGATCCTCAAGGACATGTCGGATGGCTTCATCTCTAACCTAACCATTCA
  GAGGCAGTACTTCCCTAATGATGAAGATCAGGTTGGAGCTGCTAAAGCTCTGTTTCGGCTGCAAGACACC
  TACAATCTGGACACAAATACCATCTCAAAGGGGAATCTTCCAGGAGTGCAACATAAGTCTTTCCTGACAG
  CCGAGGACTGCTTTGAGCTGGGCAAAGTGGCCTATACAGAGGCAGATTATTACCACACAGAACTGTGGAT
  GGAGCAGGCTCTGACGCAGCTGGAGGAGGGAGAGCTGTCTACTGTAGATAAAGTCTCTGTTCTGGATTAC
  CTGAGCTATGCGGTGTACCAGCAGGGAGACCTGGATAAGGCGCTTCTCCTCACCAAGAAGCTTCTTGAGC
  TAGATCCTGAACATCAGAGAGCTAATGGTAACTTAGTATATTTTGAATATATAATGAGTAAAGAAAAAGA
  TGCCAATAAGTCAGCGTCGGGCGACCAATCTGATCAGAAAACTGCACCAAAGAAAAAAGGGATAGCTGTG
  GATTACCTGCCAGAGAGACAGAAGTACGAAATGCTGTGCCGTGGGGAGGGTATCAAAATGACTCCCCGGA
  GACAGAAAAGACTGTTCTGCCGCTACCATGATGGGAACCGGAATCCTAAGTTCATCCTGGCCCCAGCCAA
  GCAGGAGGACGAGTGGGACAAGCCCCGGATTATCCGTTTCCATGACATCATCTCTGACGCGGAGATTGAG
  ATTGTCAAAGACTTAGCAAAACCAAGGCTGAGGCGAGCCACCATTTCAAACCCAGTAACAGGAGCCTTGG
  AGACGGTACATTACAGAATTAGCAAAAGTGCTTGGCTGTCTGGCTACGAAGACCCTGTGGTATCCCGAAT
  TAATATGAGAATACAAGATCTCACAGGACTGGATGTTTCTACGGCCGAGGAGTTACAGGTAGCAAATTAC
  GGAGTTGGAGGACAGTATGAGCCCCATTTTGACTTTGCAAGGAAAGATGAGCCGGATGCCTTTAGAGAGC
  TTGGGACAGGAAATAGGATTGCCACGTGGCTGTTCTATATGAGCGATGTGTCTGCTGGAGGAGCTACTGT
  TTTTCCTGAAGTGGGAGCCAGTGTTTGGCCCAAAAAAGGCACTGCTGTCTTCTGGTATAATCTGTTTGCC
  AGTGGGGAAGGAGATTACAGTACACGGCATGCAGCCTGTCCCGTGCTAGTTGGAAACAAATGGGTATCCA
  ACAAATGGCTCCATGAACGTGGACAAGAATTTCGAAGGCCGTGCACCCTGTCAGAATTGGAATGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_011030
• Insert Size: 1605 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_011030.2, NP_035160.1
• RefSeq Size: 4057 bp
• RefSeq ORF: 1605 bp
• Locus ID: 18451
• UniProt ID: Q60715
• Cytogenetics: 10 B4
• Gene Summary: Catalyzes the post-translational formation of 4-hydroxyproline in -Xaa-Pro-Gly- sequences in collagens and other proteins.[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (3) lacks one exon and contains two alternate in-frame exons in the central coding region, compared to variant 1. The encoded isoform (3) is longer than isoform 1.