Foxn4 (NM_148935) Mouse Untagged Clone

CAT#: MC217504

Foxn4 (untagged) - Mouse forkhead box N4 (Foxn4), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Foxn4
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC217504 representing NM_148935
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGATAGAAAGTGGCATTTGGTCCAGAATGTCAGAAATGATCAGAAGCTCGGGGCACAGCCACCACTGCT
  CTCCACAGGAATACAGGTTTCTGCCTCCCGTGGGTGACGACGACCTCCCTGGGGACCTTCAGTCGCTGTC
  CTGGCTCACGGCCGTGGACGTGCCTCGACTGCAGCAGATGGCAAATGGGCGGATAGACCTGGGCAGTTCA
  GGTGTGACACATCCACACCCAGGTGCCTTGGCTGGGACAGCAGACCTGCATGTGGGTGCGGCCCCACGTC
  CCCTGCTCCGCCGCAGCCAGACTGCCGTGGTCCCCAGGGGTGTGCTGGGCTTGAGCCCTATAGGCAACCA
  CAGAGCCAGCGCTGAGCAGATGAACCAGTTCCCTGCAGGAGGCCAGGCATCATCGGGCCTCCAGGAAATG
  CCACAGCTGTACTCCCCAGCTACCCAAATCCCGTTCCCTCTGCCTCTGGGGTCCCAGCAGTGCCCTCCTG
  CCGGCCTCTATGGTTCTCCGTTTTCGGCTCGGCCTTCCTACCCACAGGCCCACGGGGCAATGCATGCGTC
  TCAGGAGCCACACCCCAAACACTACCCCAAGCCCATCTACTCCTACAGCTGTCTGATCGCCATGGCCTTG
  AAGAACAGCAAAACAGGCAGCTTGCCCGTGAGTGAGATCTACAGCTTCATGAAGGAGCACTTCCCCTACT
  TCAAGACAGCACCTGACGGCTGGAAGAACTCCGTGAGGCACAACCTGTCCCTGAACAAGTGCTTCGAGAA
  GGTGGAAACCAAGTCCAGCGGCTCCTCACGCAAGGGCTGCCTGTGGGCCCTGAACCTGGCTCGCATCGAC
  AAGATGGAGGAGGAGATGCACAAGTGGAAACGAAAGGACCTCGCTGCCATACACCGCAGCATGGCCAACC
  CTGAGGAGTTGGACAAGCTCATCTCAGACCGCCCGGAAAGCTGCCGGCGTCCTGGAAAACGAGGAGAGCC
  TAAAGCCCCCATGCTAACTCATGCCACCACGGTGGCCATGGCCCACAGCTGCCTGGCCATCTCCCAACTC
  CCACCAAAGCCACTGATGACCCTGTCCCTGCAGTCAGTGCCTCTGCACCACCAGCTCCAGCCCCAGGCAC
  ACCTGGCCCCAGACTCCCCGGCACCAGCACAGACCCCACCTCTTCATGCCCTACCCAGTCTCAGCCCAGG
  GCCTCTCCCGCAGCCAGCCATGGGTAGGGTTCCTGGGGACTTCCTCAACATCAACAGTGACATGAACACA
  GAGGTGGACGCCCTGGACCCCAGCATCATGGACTTCGCTCTACAGGGTAACCTGTGGGAAGAGATGAAGG
  AGGACAGCTTCAGCCTAGACACGCTGGAGGCCTTTGGAGACTCCCCGCTTGGCTGCGACCTGGGAGCCCC
  AAGCCTGACCCCTGTCTCTGGCAACAGTGACCAGTCCTTCCCTGATGTGCAGGTGACAGGTCTCTATGCT
  GCCTACTCCACTGCCGCAGACGGTGTGGCCCCATCAGCGGCCAACTCTGCGCAGTACCTGGGTACACCAG
  GGAACAAGCCTATTGCTCTGCTTTGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_148935
• Insert Size: 1566 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_148935.2, NP_683737.2
• RefSeq Size: 2970 bp
• RefSeq ORF: 1566 bp
• Locus ID: 116810
• UniProt ID: Q8K3Q3
• Cytogenetics: 5 F
• Gene Summary: Transcription factor essential for neural and some non-neural tissues development, such as retina and lung respectively. Binds to an 11-bp consensus sequence containing the invariant tetranucleotide 5'-ACGC-3'. During development of the central nervous system, is required to specify the amacrine and horizontal cell fates from multipotent retinal progenitors while suppressing the alternative photoreceptor cell fates through activating DLL4-NOTCH signaling. Also acts synergistically with ASCL1/MASH1 to activate DLL4-NOTCH signaling and drive commitment of p2 progenitors to the V2b interneuron fates during spinal cord neurogenesis. In development of non-neural tissues, plays an essential role in the specification of the atrioventricular canal and is indirectly required for patterning the distal airway during lung development.[UniProtKB/Swiss-Prot Function]