Bbs4 (NM_175325) Mouse Untagged Clone

CAT#: MC217495

Bbs4 (untagged) - Mouse Bardet-Biedl syndrome 4 (human) (Bbs4), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Bbs4
• Synonyms: AW537059; AW742241; D9Ertd464e
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC217495 representing NM_175325
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCTGAAGTGAAGCTTGGGATGAAAACTCAGGTTCCTGCATCTGTTGAATCTCAAAAACCTAGGTCAA
  AAAAAGCTCCAGACTTCCCTATTGTGGAGAAACAGAACTGGCTGATCCATCTCCACTATATCCGGAAGGA
  TTATGAAGCTTGCAAGGCTGTGATCAAAGAACAGCTTCAGGAGACTCAGGGGCTATGTGAATATGCTATC
  TATGTCCAAGCACTGATTTTTCGCCTGGAAGGAAATATCCAAGAATCCCTAGAACTCTTTCAGACATGTG
  CTGTTCTCAGCCCACAGTGTGCTGATAATCTCAAGCAGGTGGCCAGATCTTTGTTTCTTCTGGGAAAACA
  CAAAGCTGCCACTGAAGTATATAATGAAGCAGCTAAACTTAACCAGAAAGACTGGGAGATCTGCCATAAC
  CTGGGAGTGTGCTACACTTACCTGAAGCAGTTCAACAAGGCACAAGACCAACTGCACAGTGCCCTCCAGC
  TTAACAAGCATGACCTGACTTACATAATGTTGGGGAAGATCCATTTACTGCAGGGAGACCTGGATAAAGC
  CATCGAGATCTACAAGAAAGCAGTAGAGTTCTCACCAGAAAATACTGAACTTCTTACAACTTTAGGATTG
  CTCTACTTGCAGCTTGGTGTGTACCAGAAGGCATTTGAACATCTTGGGAATGCACTGACCTACGACCCTG
  CCAACTACAAGGCCATCTTGGCAGCAGGCAGCATGATGCAGACTCATGGGGACTTTGATGTCGCCCTTAC
  CAAATACAGAGTTGTAGCCTGTGCTATTCCAGAAAGTCCTCCACTTTGGAATAACATTGGAATGTGTTTC
  TTTGGCAAGAAGAAATATGTGGCAGCTATCAGCTGCCTAAAACGAGCCAACTACTTGGCACCCTTCGACT
  GGAAGATTCTGTACAACCTGGGCCTTGTCCATTTGACTATGCAGCAGTATGCATCAGCATTCCATTTTCT
  CAGTGCAGCCATCAACTTCCAGCCAAAGATGGGGGAACTCTACATGCTCTTGGCTGTGGCTCTGACCAAC
  CTGGAAGATATAGAGAATGCCAGAAGAGCTTATGTAGAAGCTGTTCGCCTGGATAAGTGTAACCCTTTAG
  TAAACCTGAACTATGCTGTGCTGCTGTATAACCAGGGTGAGAAGAAGAGCGCCCTGGCTCAGTACCAGGA
  GATGGAGAAGAAGGTCAACTTTCTCAAGGACAACAGCCCTCTGGAATTTGACTCTGAGATGGTAGAGATG
  GCTCAGAAGTTGGGAGCTGCCCTTCAGGTGGGAGAGGCACTGGTCTGGACCAAACCAGTCAAAGATCCCA
  AGACAAAGCACCGGACCAATTCAGGCAGCAAATCTGCTACTCTCCAGCAGCCTCTGGGTTCCATTCAAGC
  TCTAGGACAGGCGATGTCTTCAGCAGCTGCATACAGAAAGATCCTTTCAGGTGCTGTAGGAGCCCAGCTC
  CCAAAGCCACCATCACTGCCACTGGAGCCAGAGCCAGAGCCCACTGTGGAAGCAAGTCCAACAGAAGCAT
  CAGAACAAAAGAAAGAAAAATAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_175325
• Insert Size: 1563 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_175325.3, NP_780534.1
• RefSeq Size: 2542 bp
• RefSeq ORF: 1563 bp
• Locus ID: 102774
• UniProt ID: Q8C1Z7
• Cytogenetics: 9 32.01 cM
• Gene Summary: The BBSome complex is thought to function as a coat complex required for sorting of specific membrane proteins to the primary cilia. The BBSome complex is required for ciliogenesis but is dispensable for centriolar satellite function. This ciliogenic function is mediated in part by the Rab8 GDP/GTP exchange factor, which localizes to the basal body and contacts the BBSome. Rab8(GTP) enters the primary cilium and promotes extension of the ciliary membrane. Firstly the BBSome associates with the ciliary membrane and binds to RAB3IP/Rabin8, the guanosyl exchange factor (GEF) for Rab8 and then the Rab8-GTP localizes to the cilium and promotes docking and fusion of carrier vesicles to the base of the ciliary membrane. The BBSome complex, together with the LTZL1, controls SMO ciliary trafficking and contributes to the sonic hedgehog (SHH) pathway regulation. Required for proper BBSome complex assembly and its ciliary localization. Required for microtubule anchoring at the centrosome but not for microtubule nucleation. May be required for the dynein-mediated transport of pericentriolar proteins to the centrosome (By similarity).[UniProtKB/Swiss-Prot Function]