Nup62 (NM_053074) Mouse Untagged Clone

CAT#: MC217430

Nup62 (untagged) - Mouse nucleoporin 62 (Nup62), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Nup62
• Synonyms: D7Ertd649e; Nupc1; p62
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC217430 representing NM_053074
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAGTGGATTTAACTTTGGAGGCACCGGGGCTCCTGCTGGCGGCTTTACATTTGGGACCGCAAAGACTG
  CGACCACCACACCTGCCACTGGCTTTTCCTTCTCTGCTTCTGGCACCGGCACCGGAGGGTTTAATTTTGG
  GACTCCCTCCCAGCCAGCTGCGACCACCCCTTCCACCAGCCTCTTCTCACTCACCACACAGACCCCAACC
  ACACAGACCCCAGGATTCAACTTTGGAACAACACCTGCTTCTGGAGGAACAGGCTTCTCCCTGGGGATCA
  GCACCCCAAAGCTCAGCCTGAGTAACGCAGCTGCCACACCAGCCACGGCCAACACTGGCAGCTTTGGGCT
  TGGCAGCAGCACCCTCACCAATGCCATCTCAAGTGGCAGCACCTCCAACCAGGGGACAGCCCCCACTGGC
  TTTGTCTTTGGCTCTTCTACCACCTCTGCTCCGTCCACCGGCTCCACTGGGTTCTCATTCACCAGTGGCA
  GTGCATCCCAGCCTGGAGCCTCTGGCTTCAGCCTTGGCTCTGTGGGTAGCTCTGCCCAGCCCACAGCACT
  GTCTGGCTCTCCCTTCACACCAGCCACTCTGGTGACTACTACAGCAGGAGCTACACAGCCAGCTGCTGCT
  GCACCCACTGCTGCCACCACCAGTGCAGGGTCTACACTCTTTGCTTCCATAGCTGCTGCTCCTGCCTCAT
  CCAGTGCTACAGGGCTCTCCCTCCCAGCTCCGGTGACAACTGCAGCAACTCCTAGTGCTGGGACTTTGGG
  CTTCAGCCTCAAGGCCCCTGGAGCAGCTCCTGGTGCCTCCACCACCAGCACCACCACTACCACTACCACC
  ACTACTACCACTGCTGCTGCCGCTGCCGCCTCTACCACAACCACTGGCTTTGCCTTAAGTCTGAAACCCC
  TGGTGTCAGCTGGCCCCAGCAGTGTAGCAGCTACTGCTCTGCCTGCCTCCAGCACAGCAGCTGGGACTGC
  CACAGGTCCAGCGATGACCTATGCACAGCTGGAAAGCTTGATCAACAAGTGGAGTCTGGAGCTGGAAGAT
  CAGGAGCGGCACTTCCTGCAGCAGGCCACACAGGTCAATGCCTGGGACCGCACACTGATTGAGAATGGGG
  AGAAGATCACCAGCCTGCACCGAGAGGTGGAAAAGGTGAAGCTGGATCAGAAGCGACTGGACCAGGAGCT
  GGACTTTATCCTGTCGCAGCAGAAGGAGCTGGAAGACCTCCTGAGCCCATTGGAGGAGTCAGTGAAGGAG
  CAAAGTGGCACCATATACCTTCAGCACGCTGATGAGGAGCGTGAGAAGACCTACAAGCTGGCTGAGAACA
  TTGATGCTCAGCTCAAGCGCATGGCACAGGACCTCAAGGATATCATCGAGCACCTGAACATGGCTGGTGG
  CCCTGCAGACACCAGTGACCCATTGCAACAGATCTGCAAGATCCTCAATGCACACATGGACTCTCTTCAG
  TGGGTGGACCAGAGCTCTGCCCTGCTGCAGAGGAGGGTGGAGGAGGCTAGCCGTGTATGTGAGGGCCGGC
  GCAAGGAACAGGAGCGCAGTCTGCGCATTGCCTTTGACTAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_053074
• Insert Size: 1581 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_053074.2, NP_444304.1
• RefSeq Size: 3746 bp
• RefSeq ORF: 1581 bp
• Locus ID: 18226
• UniProt ID: Q63850
• Cytogenetics: 7 28.93 cM
• Gene Summary: Essential component of the nuclear pore complex. The N-terminal is probably involved in nucleocytoplasmic transport. The C-terminal is involved in protein-protein interaction probably via coiled-coil formation, promotes its association with centrosomes and may function in anchorage of p62 to the pore complex. Plays a role in mitotic cell cycle progression by regulating centrosome segregation, centriole maturation and spindle orientation. It might be involved in protein recruitment to the centrosome after nuclear breakdown.[UniProtKB/Swiss-Prot Function]