Mitf (NM_001113198) Mouse Untagged Clone

CAT#: MC217428

Mitf (untagged) - Mouse microphthalmia-associated transcription factor (Mitf), transcript variant 1, (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Mitf
• Synonyms: BCC2; Bhlhe32; bw; Gsfbcc2; mi; vit; Vitiligo; Wh
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC217428 representing NM_001113198
  Red=Cloning site Blue=ORF
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCAGTCCGAATCGGGAATCGTGGCGGATTTCGAAGTCGGGGAGGAGTTTCACGAAGAACCCAAAACCT
  ATTACGAACTCAAAAGTCAACCTCTGAAGAGCAGCAGTTCTGCAGAGCATTCTGGGGCCTCCAAGCCTCC
  GTTAAGCTCCTCCACTATGACATCACGCATCTTGCTACGCCAGCAACTCATGCGTGAGCAGATGCAGGAG
  CAGGAGCGCAGGGAGCAGCAGCAGAAGCTGCAGGCAGCCCAGTTCATGCAACAGAGAGTGGCCGTGAGTC
  AGACACCAGCCATAAACGTCAGCGTGCCCACCACCCTTCCCTCTGCCACCCAGGTGCCGATGGAAGTCCT
  TAAGGTGCAGACCCACCTGGAAAACCCCACCAAGTACCACATACAGCAAGCTCAGAGGCACCAGGTAAAG
  CAGTACCTTTCTACCACTTTAGCAAATAAACATGCCAGCCAAGTCCTGAGCTCACCATGTCCAAACCAGC
  CTGGCGACCATGCCATGCCACCAGTGCCGGGGAGCAGCGCACCCAACAGCCCTATGGCTATGCTCACTCT
  TAACTCCAACTGTGAAAAAGAGGCATTTTATAAGTTTGAGGAGCAGAGCAGGGCAGAGAGTGAGTGCCCA
  GGTATGAACACGCACTCTCGAGCGTCGTGCATGCAGATGGATGATGTAATTGATGACATCATCAGCCTGG
  AATCAAGTTATAATGAAGAAATTTTGGGCTTGATGGATCCGGCCTTGCAAATGGCAAATACGTTACCCGT
  CTCTGGAAACTTGATCGACCTCTACAGCAACCAGGGCCTGCCACCGCCAGGCCTTACCATCAGCAACTCC
  TGTCCAGCCAACCTTCCCAACATAAAAAGGGAGCTCACAGCGTGTATTTTCCCCACAGAGTCTGAAGCAA
  GAGCATTGGCTAAAGAGAGGCAGAAAAAGGACAATCACAACTTGATTGAACGAAGAAGAAGATTTAACAT
  AAACGACCGCATTAAGGAGCTAGGTACTCTGATCCCCAAGTCAAATGATCCAGACATGCGGTGGAACAAG
  GGAACCATTCTCAAGGCCTCTGTGGACTACATCCGGAAGTTGCAACGGGAACAGCAACGAGCTAAGGACC
  TTGAAAACCGACAGAAGAAGCTGGAGCATGCGAACCGGCACCTGCTGCTCAGAGTACAGGAGCTGGAGAT
  GCAGGCTAGAGCGCATGGACTTTCCCTTATCCCATCCACCGGTCTCTGCTCGCCTGATCTGGTGAATCGG
  ATCATCAAGCAAGAACCAGTTCTTGAGAACTGCAGCCAGGAACTTGTACAGCACCAGGCAGACCTGACAT
  GTACGACAACTCTGGATCTCACGGACGGTACCATCACCTTTACCAACAACCTCGGCACCATGCCGGAGAG
  CAGCCCGGCCTACAGCATCCCCAGGAAGATGGGCTCCAACTTGGAAGACATCCTGATGGACGATGCCCTC
  TCACCTGTTGGAGTCACCGACCCACTGCTGTCATCAGTGTCGCCAGGAGCTTCAAAAACAAGCAGCCGGA
  GGAGCAGTATGAGCGCAGAAGAAACGGAGCATGCGTGTTAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001113198
• Insert Size: 1581 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001113198.1, NP_001106669.1
• RefSeq Size: 4890 bp
• RefSeq ORF: 1581 bp
• Locus ID: 17342
• UniProt ID: Q08874
• Cytogenetics: 6 45.05 cM
• Gene Summary: This transcription factor serves at a critical point between extracellular signaling and downstream targets in cell specification in early eye and neural crest development. Mutant alleles have been identified that generate distinct phenotypes. Some of these alleles are being used to model the human diseases Waardenburg syndrome IIa and Tietz syndrome. [provided by RefSeq, Jul 2008]
  Transcript Variant: This variant (1) represents the longest transcript and encodes the longest isoform (1, also known as Mitf-A). Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.