Cyp4x1 (NM_001003947) Mouse Untagged Clone

CAT#: MC217187

Cyp4x1 (untagged) - Mouse cytochrome P450, family 4, subfamily x, polypeptide 1 (Cyp4x1), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Cyp4x1
• Synonyms: A230025G20; Cyp4a28-ps; CYPIVX1; CYP_a
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC217187 representing NM_001003947
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAGGCCTCCTGGCTGGAGACTCGTTGGGCGCGGCCCTTGCACCTGGCTTTGGTGTTCTGCCTGGCGC
  TGGTGCTGATGCAGGCCATGAAGCTCTACCTGCGGAGACAGCGACTGCTGCGCGACCTGAGCCCCTTCCC
  GGGGCCTCCCGCCCACTGGCTCCTGGGACACCAGAAGTTTCTTCAGGAGGATAATATGGAGACGCTTGAT
  GAGATTGTCAAAAAGCACCCTTGTGCCTTCCCCTGCTGGGTAGGGCCCTTCCAGGCATTTTTCTACATCT
  ATGACCCAGACTATGCGAAGATATTTCTGAGCAGAACAGACCCAAAGATGCAGTATCTGCACCAGCTCCT
  GACTCCATGCATTGGACGAGGACTCCTGAATTTAGATGGGCCCAGGTGGTTCCAACACCGCTGCCTCCTA
  ACTCCTGCATTCCATCAGGACATCCTAAAACCATGTGTGGACACGATGGCCCACTCTGTGAAAGTGATGC
  TGGATAAATGGGAGAAGATGTGGACCACTCAGGAAACAACCATCGAGGTTTTTGAACACATCAACTTGAT
  GACCCTGGACATAATAATGAAATGTGCTTTTGGCCAGGAGACCAACTGCCAGATAAACGGCACCTATGAG
  TCTTACGTGAAGGCAACATTTGAACTTGGTGAAATCATATCTTCTCGCTTGTACAATTTCTGGCATCATC
  ATGACATAATTTTCAAACTCAGTCCTAAGGGCCACTGCTTCCAGGAGTTAGGCAAAGTGATACATCAATA
  CACAGAAAAGATAATCCAGGACAGAAAGAAAATCCTCAAGAATCAAGTAAAGCAGGATGACACTCAGACG
  TCTCAAATTTTTCTGGATATTGTTCTTTCTGCACAGGCTGAAGATGAACGAGCCTTCTCAGATGCTGACC
  TTCGGGCTGAGGTGAACACCTTCATGTGGGCAGGACATGATGCCTCTGCAGCTAGCATCTCCTGGCTCCT
  TTACTGCCTGGCTCTAAATCCCGAGCATCAGGACAGATGCCGGACAGAGATCAGGAGCATCCTGGGAGAT
  GGGTCTTCCATCACCTGGGAACAGCTGGATGAGATGTCATACACCACAATGTGCATCAAGGAGACACTCC
  GCTTGATTCCTCCTGTCCCATCCATCTCCAGGGAGCTCAGCAAACCCCTTACCCTCCCAGATGGACACTC
  ACTGCCTGCAGGTATGACTGTGGTTCTTAGTATTTGGGGTCTCCACCACAACCCTGCTGTCTGGAACGAC
  CCAAAGGTCTTTGACCCCTTGAGATTCACTAAGGAGAATTCTGATCAGAGACACCCCTGTGCCTTCCTAC
  CATTCTCCAGTGGTCCAAGGAACTGCATCGGGCAGCAGTTTGCCATGCTGGAGCTAAAGGTGGCCATTGC
  CTTGATTCTGCTCCACTTTCAAGTAGCTCCAGACCTCACCAGGCCTCCTGCCTTCTCCAGCCACACTGTC
  CTCAGACCCAAGCATGGAATCTATTTGCACCTGAAGAAACTCCTTGAGTGTTAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001003947
• Insert Size: 1524 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001003947.1, NP_001003947.1
• RefSeq Size: 1524 bp
• RefSeq ORF: 1524 bp
• Locus ID: 81906
• UniProt ID: Q6A152
• Cytogenetics: 4 D1
• Gene Summary: A cytochrome P450 monooxygenase that selectively catalyzes the epoxidation of the last double bond of the arachidonoyl moiety of anandamide, potentially modulating endocannabinoid signaling. Has no hydroxylase activity toward various fatty acids, steroids and prostaglandins. Mechanistically, uses molecular oxygen inserting one oxygen atom into a substrate, and reducing the second into a water molecule, with two electrons provided by NADPH via cytochrome P450 reductase (CPR; NADPH-ferrihemoprotein reductase).[UniProtKB/Swiss-Prot Function]