Foxo4 (NM_018789) Mouse Untagged Clone

CAT#: MC217164

Foxo4 (untagged) - Mouse forkhead box O4 (Foxo4), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Foxo4
• Synonyms: afx; Afxh; Mllt7
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC217164 representing NM_018789
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGATCCAGAGAATAAGAAGTCAGCCACAGGGGCTGCCGCGATCCTAGACCTCGATCCCGACTTCGAAC
  CCCAGAGCCGTCCGCGATCCTGCACCTGGCCCCTTCCTCGACCAGACCTCGCTACGGAGCCACACGAACC
  GTCCGAGGTGGAGCCCAGTCTGGGACAGAAGGTACCCACGGAGGGACACTCCGAACCGATCCTGTTGCCC
  TCTCGGCTCCCAGAGCCGGCAGGGGGCCCCCAGCCGGGAATCCTGGGGGCTGTAACAGGTCCTCGGAAGG
  GAGGCTCCCGCCGGAATGCCTGGGGAAATCAGTCATATGCAGAACTCATCAGCCAGGCCATTGAAAGCGC
  CCCGGAGAAGCGGCTGACACTCGCCCAGATCTACGAATGGATGGTCCGCACGGTGCCCTACTTCAAGGAC
  AAGGGTGACAGCAACAGCTCGGCAGGATGGAAGAACTCCATCCGTCACAACCTGTCCTTGCACAGCAAGT
  TCATCAAGGTTCACAACGAGGCCACTGGCAAGAGCTCTTGGTGGATGCTGAACCCCGATGGCGGCAAGGG
  TGGCAAGGCACCCCGCCGCAGGGCTGCCTCCATGGATAGCAGCAGCAAGCTGCTCCGGGGCCGCAGCAAA
  GGCCCCAAGAAGAAGCCGTCTGTCCTGCCAGCTCCACCGGAAGGTGCCACGCCAAGGAGCCCTCTGGGCC
  ACTTTGCCAAGTGGTCAAGCAGCCCTTGTCCTCGAAATCGAGAAGAAGCTGATGTGTGGACCACTTTCCG
  TCCACGAAGCAGTTCAAATGCTAGCACCGTCAGCACCCGGCTGTCGCCAATGAGGCCGGAGTCTGAAGTG
  CTAGCAGAAGAGGAAATGCCAGCCTCGGCCAGCAGCTATGCAGGGGGGGTCCCTCCCACGCTCAGTGAAG
  ATCTAGAGCTACTGGATGGGCTCAATCTCGCATCTCCCCATTCCTTGCTCTCTAGGAGCGGTCTCTCCGG
  CTTCTCTTTGCAGCATCCTGGGCTTGCTGGCCCCTTACATAGCTATGGTGCCTCCCTCTTTGGCCCAATA
  GATGGGTCTTTGTCAGCAGGAGAAGGGTGCTTCTCAAGTTCCCAGTCTCTAGAGGCTCTGCTCACCTCTG
  ATACACCACCACCTCCTGCTGATGTCCTCATGACCCAGGTAGATCCTATCCTGTCTCAGGCTCCTACACT
  TCTGTTACTGGGAGGAATGCCTTCCTCTAGCAAGCTGGGCACAGGAGTCAGCCTGTGTCCTACGCCGCTA
  GAGGGTCCTGGTCCCAGCAACCTGGTCCCCAACCTTTCTGTGATGGCACCACCCCCAGTCATGGCAGGCG
  CTCCCATCCCTAAGGTCCTGGGAACCCCTGTGCTCGCATCTCCTACTGAAGATTCCAGCCATGACAGAAT
  GCCTCAGGATCTGGATCTTGATATGTATATGGAGAACCTGGAGTGCGACATGGATAACATCATCAGTGAC
  CTCATGGATGGTGAGGGACTGGACTTCAACTTTGAGCCAGATCCCTGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_018789
• Insert Size: 1518 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_018789.2, NP_061259.1
• RefSeq Size: 3162 bp
• RefSeq ORF: 1518 bp
• Locus ID: 54601
• UniProt ID: Q9WVH3
• Cytogenetics: X 43.89 cM
• Gene Summary: Transcription factor involved in the regulation of the insulin signaling pathway. Binds to insulin-response elements (IREs) and can activate transcription of IGFBP1. Down-regulates expression of HIF1A and suppresses hypoxia-induced transcriptional activation of HIF1A-modulated genes. Also involved in negative regulation of the cell cycle. Involved in increased proteasome activity in embryonic stem cells (ESCs) by activating expression of PSMD11 in ESCs, leading to enhanced assembly of the 26S proteasome, followed by higher proteasome activity (By similarity). Represses smooth muscle cell differentiation by inhibiting the transcriptional coactivator activity of myocardin.[UniProtKB/Swiss-Prot Function]