Phf10 (NM_024250) Mouse Untagged Clone

CAT#: MC216937

Phf10 (untagged) - Mouse PHD finger protein 10 (Phf10), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Phf10
• Synonyms: 1810055P05Rik; AV024533
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC216937 representing NM_024250
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCAGCGGCCGGGCCCGGGGCGGCGCTGTCCCCGGGGCGGTGCGACAGCGACCCGGCCTCCCCCGGAG
  CGCAGTCCCCAAAGGATGATAATGAAGATAACTCAAATGATGGGACCCATCCATGTAAAAGGAGGCGAAT
  GGGCTCAGGAGACAGCTCAAGAAGTTGTGAGACTTCAAGTCAAGATCTTAGCTTCAGTTACTACCCAGCA
  GAAAACTTAATCGAATACAAATGGCCACCTGATGAAACAGGAGAATACTATATGCTTCAGGAGCAAGTCA
  GTGAATATCTGGGTGTGACCTCCTTCAAGCGGAAATATCCAGATTTAGAGCGACGAGATTTATCTCACAA
  GGAGAAACTATACCTGAGAGAATTAAACGTCATCACGGAAACACAGTGCACACTGGGTTTAACAGCATTG
  CGCAGTGATGAAGTGATTGACTTAATGATAAAAGAATATCCAGCTAAACACGCTGAATATTCGGTTATCC
  TACAAGAAAAGGAACGTCAGAGAATTACAGATCATTATAAAGAGTATTCTCAAATGCAACAACAGAGTAC
  TCAGAAAGTCGAAGCCAGCAAAGTACCTGAGTACATTAAGAAAGCAGCCAAGAAGGCAGCTGAGTTCAAC
  AGCAACTTAAACCGGGAGCGCATGGAAGAAAGAAGAGCCTATTTTGACTTACAGACACATGTTATCCAAG
  TGCCTCAAGGAAAGTACAAAGTGTTGCCGACAGACCGAACGAAGGTCAGTTCCTACCCAGTGGCTCTCAT
  CCCGGGACAGTTCCAGGAGTATTATAAGAGGTACTCACCAGATGAGCTTCGGTACTTGCCATTAAACACA
  GCCCTGTATGAGCCGCCCCTGGACCCAGAGCTCCCGGCACTAGATAGTGATGGAGACTCAGATGATGGCG
  AAGATGGCGGAGGGGATGAGAAGCGGAAGAATAAAGGCACTTCGGACAGCTCCTCAGGCAATGTGTCTGA
  AGGAGACAGCCCCCCTGACAGCCAGGAGGACACCTTCCACGGAAGACAGAAATCAAAAGACAAAATGGCC
  ACTCCAAGAAAAGACGGCTCCAAACGTTCTGTACTGTCCAAATCAGCTCCTGGGTACAAGCCAAAGGTCA
  TTCCAAATGCTCTATGTGGAATTTGTCTGAAGGGTAAGGAGTCCAACAAGAAAGGAAAGGCTGAATCACT
  TATACACTGCTCCCAGTGTGATAACAGTGGCCACCCTTCTTGCTTGGATATGACCATGGAGCTTGTTTCT
  ATGATTAAGACCTACCCATGGCAGTGTATGGAATGTAAGACATGCATTATATGTGGACAGCCCCACCATG
  AAGAAGAAATGATGTTCTGTGATGTGTGTGACAGAGGTTATCATACTTTTTGTGTGGGCCTTGGTGCTAT
  TCCTTCAGGTCGCTGGATTTGTGACTGTTGTCAGCGAGCTCCCCCAACACCCAGGAAAGTGGGCAGAAGG
  GGGAAAAACAGCAAAGAGGGGTAA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_024250
• Insert Size: 1494 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_024250.4, NP_077212.3
• RefSeq Size: 1665 bp
• RefSeq ORF: 1494 bp
• Locus ID: 72057
• UniProt ID: Q9D8M7
• Cytogenetics: 17 A2
• Gene Summary: Involved in transcription activity regulation by chromatin remodeling. Belongs to the neural progenitors-specific chromatin remodeling complex (npBAF complex) and is required for the proliferation of neural progenitors. During neural development a switch from a stem/progenitor to a post-mitotic chromatin remodeling mechanism occurs as neurons exit the cell cycle and become committed to their adult state. The transition from proliferating neural stem/progenitor cells to post-mitotic neurons requires a switch in subunit composition of the npBAF and nBAF complexes. As neural progenitors exit mitosis and differentiate into neurons, npBAF complexes which contain ACTL6A/BAF53A and PHF10/BAF45A, are exchanged for homologous alternative ACTL6B/BAF53B and DPF1/BAF45B or DPF3/BAF45C subunits in neuron-specific complexes (nBAF). The npBAF complex is essential for the self-renewal/proliferative capacity of the multipotent neural stem cells. The nBAF complex along with CREST plays a role regulating the activity of genes essential for dendrite growth.[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (1) encodes the longer isoform (1).