Gdf5 (NM_008109) Mouse Untagged Clone

CAT#: MC216933

Gdf5 (untagged) - Mouse growth differentiation factor 5 (Gdf5), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Gdf5
• Synonyms: b; BMP-14; bp; brp; CDMP-; Cdmp-1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC216933 representing NM_008109
  Red=Cloning site Blue=ORF
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAGACTCCCCAAACTCCTCACTCTTTTGCTGTGGCACCTGGCTTGGCTGGACCTGGAACTCATCTGCA
  CTGTGCTGGGTGCCCCTGACTTAGGACAGAGAACCCCAGGGGCCAAGCCAGGGTTGACCAAAGCGGAGGC
  CAAGGAGAGGCCACCCCTGGCCAGGAATGTCTTTAGGCCAGGGGGTCATATCTATGGTGTGGGGGCCACC
  AATGCCAGGGCCAAGGGAAGCTCTGGGCAGACACAGGCCAAGAAGGATGAACCCAGAAAGATGCCCCCCA
  GATCCGGTGGCTCTGAAACCAAGCCAGGACCCTCTTCCCAGACTAGACAGGCTGCAGCCCGGACTGTAAC
  CCCAAAAGGACAGCTTCCTGGGGGCAAAGCATCTTCAAAAGCAGGATCTGCCCCCAGCTCCTTCCTGCTG
  AAGAAGACCAGGGAGCCTGGGACCCCTCGAGAGCCCAAGGAGCCGTTCCGCCCGCCCCCCATCACACCCC
  ACGAATACATGCTCTCCCTGTACAGGACGCTGTCCGATGCTGACAGAAAGGGAGGTAACAGCAGCGTGAA
  GTTGGAGGCTGGCCTGGCCAACACCATCACCAGCTTTATTGACAAAGGGCAAGATGACCGAGGCCCTGCG
  GTCAGGAAGCAGAGGTACGTGTTTGACATCAGTGCCTTGGAGAAGGATGGGCTGTTGGGGGCTGAACTGC
  GGATCTTACGGAAGAAGCCCTTGGACGTGGCCAAGCCAGCGGTCCCCAGTAGCGGGCGGGTTGCCCAACT
  GAAGCTGTCCAGCTGCCCCAGCGGCCGGCAGCCGGCAGCCTTGCTGGATGTGCGCTCCGTGCCAGGCCTG
  GATGGATCTGGCTGGGAGGTGTTCGACATCTGGAAGCTCTTCCGAAATTTTAAGAACTCAGCGCAGCTGT
  GCCTGGAGCTGGAGGCCTGGGAACGGGGCCGGGCCGTGGACCTCCGTGGCCTGGGCTTTGAACGCACTGC
  CCGACAGGTCCACGAGAAAGCCTTGTTCCTAGTGTTTGGTCGTACCAAGAAACGGGACCTGTTCTTTAAT
  GAGATTAAGGCCCGCTCTGGCCAGGATGACAAGACTGTGTATGAATATTTGTTCAGCCAGCGGCGGAAAC
  GCCGGGCCCCATTGGCCAATCGCCAGGGCAAGCGACCCAGCAAGAACCTCAAGGCTCGCTGCAGTCGCAA
  GGCCTTGCATGTCAACTTCAAGGACATGGGCTGGGACGACTGGATCATCGCACCTCTTGAGTATGAGGCC
  TTCCACTGCGAAGGACTGTGTGAGTTCCCCTTGCGCTCCCACTTGGAGCCCACAAACCACGCAGTCATTC
  AGACCCTAATGAACTCTATGGACCCTGAATCCACACCACCCACTTGTTGTGTGCCTACACGGCTGAGTCC
  TATTAGCATCCTCTTCATCGACTCTGCCAACAACGTGGTGTATAAACAGTACGAGGACATGGTCGTGGAA
  TCTTGTGGCTGCAGGTAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_008109
• Insert Size: 1488 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: BC034546, AAH34546
• RefSeq Size: 2371 bp
• RefSeq ORF: 1488 bp
• Locus ID: 14563
• UniProt ID: P43027
• Cytogenetics: 2 77.26 cM
• Gene Summary: This gene encodes a secreted ligand of the TGF-beta (transforming growth factor-beta) superfamily of proteins. Ligands of this family bind various TGF-beta receptors leading to recruitment and activation of SMAD family transcription factors that regulate gene expression. The encoded preproprotein is proteolytically processed to generate each subunit of the disulfide-linked homodimer. This protein regulates the development of numerous tissue and cell types, including cartilage, joints, brown fat, teeth, and the growth of neuronal axons and dendrites. Mice with a mutation in this gene exhibit enhanced tooth enamel formation. [provided by RefSeq, Aug 2016]