Spata6 (NM_026470) Mouse Untagged Clone

CAT#: MC216837

Spata6 (untagged) - Mouse spermatogenesis associated 6 (Spata6), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Spata6
• Synonyms: 1700062C23Rik; AI790763; Hash; KRP; Mash
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC216837 representing NM_026470
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCCGAAGGTGAAGGCGCTGCAATGCGCCCTGGCGCTGGAGATCCGCTCTGTAACTTGTCCAGGAGTGG
  TGCTGAAAGACAAGGAGGACATCTATCTTAGTATCTGTGTGTTTGGCCAATACAAAAAGACACAATGTGT
  CCCAGCCACTTTTCCCCTGGTCTTCAATGCCAGAATGGTGTTTGAAAAGGTGTTCCCGGAAGCAGTAGAC
  CCTGGAGATGTGGTCGCACAGCTTGAGTATGATACAGCAGTGTTTGAGTTGATACAGCTAGTTCCACCAG
  TGGGAGAAACACTGTCTACCTATGATGAAAATACACGAGATTTCATGTTCCCGGGTCCAAACCAAATGTC
  TGGACACCATGATTCAAACCGCCAGGTTACCATGAGGAGGATTTCTGGCCTAAGAGGAATTGCTCCAAAG
  CTGGAATTTTCTACAACTTCAGTGATCACTGAATGTCTGATAAGCTCAAGGAAGTGCCGCACTCAGGATA
  AATTTACTTACCATTCAGCTCCAGTTGAAAAATCCCACGGCAGACTGCAATGCAGAACATCAAGATCACA
  GAAGAAAAAGTCCAAGTCACCCGAGAGGAGTAAATATTGCATAAACACCAAGAACTACGAACAGCCTACG
  ATTTCCTCAAAGTCACACTCTCCATCTCCCTACACAAAAAGACGGATGTGTGAGCTTTCCGAAGACACTC
  GGCGGCGGCTGGCCCATTTAAATCTGGGACCCTATGAATTCAAAAAGGAAACAGATAAGCCTCCATTTGT
  GATTAGACATGTTGATCCCCCGAGTCCCAGAACTGACAATTTTTTTGGATCACCTGGCAGAGACTGTGAA
  CGAGATGGATGGGTCAGGATGCACAGCGATCATCCTCATATTGGCTGCTGCAGATCCAAGGACTACAAGG
  TTATCAGGTCACCACATGGGAGAGACTTTGAGGATCCCTTTGAAAGGTGTGAGGAATATTTGAGCCCAAG
  GACATGTAGCAAGCCCCAGCATTCTGCAAGGACCTTACTAGTCCATTCAGCACCTTCAACAACACCAAAG
  CATTGTGCAAGCCCTGTGCTAAATAGAGCCTCTCTCAGGGAAAGATTCCACTCTGATTGGTGTTCACCTC
  CGAACTGTGATGAGATCCATGACCGGGTAAAAGATGTCTTGAAATCACATCAAGCTCATGCAAGACATTT
  ATGTGATGAGAGAGACCCAGAGAGAGAAGACGAACTGGAACTGAAAAGAAGTCTTTTATATAGAGACTCT
  GCTTATGACAGTGACCCTGAGTATAGCTCCTTTCAGCGGCCGCGTGGCTCTTTCCATTTGGATGATGGCG
  AATGCTGGTCCAACAGAGCAGCCTCTTGTAAAGGGAAATCCCACCGACCCGTCTTTGAGAACAGCATGGA
  CAAGATGTACAGGAACTTATACAAAAAAGCCTGTAGTTCTGTTTCTCATACACAGGAAAGCTTCTGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_026470
• Insert Size: 1467 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_026470.3, NP_080746.3
• RefSeq Size: 2454 bp
• RefSeq ORF: 1467 bp
• Locus ID: 67946
• UniProt ID: Q3U6K5
• Cytogenetics: 4 D1
• Gene Summary: Required for formation of the sperm connecting piece during spermiogenesis. Sperm connecting piece is essential for linking the developing flagellum to the head during late spermiogenesis (PubMed:12771232, PubMed:25605924). May be involved in myosin-based microfilament transport through interaction with myosin subunits (PubMed:25605924).[UniProtKB/Swiss-Prot Function]