Fdxr (NM_007997) Mouse Untagged Clone

CAT#: MC216770

Fdxr (untagged) - Mouse ferredoxin reductase (Fdxr), nuclear gene encoding mitochondrial protein, (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Fdxr
• Synonyms: AR
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC216770 representing NM_007997
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCTCCTCGCTGCTGGCACTGGTGGCGCTGGTCCGCGTGGTCTGGGCTTCGGCCGTCTCCCTCCAGGA
  GCACTCCGACCCCAGGCTTCTGCCAGAAGTTCTCCACACAGGAGAAGACCCCTCAGATCTGTGTGGTCGG
  CAGTGGCCCAGCTGGCTTCTACACAGCCCAACACTTGTTAAAGCACCACACCCATGCCCACGTAGACATC
  TACGAGAAGCAGCTCGTGCCATTCGGCCTGGTGCGCTTTGGTGTGGCACCTGACCATCCTGAAGTAAAGA
  ATGTTATCAACACATTTACACAGACAGCCCGCTCAGACCGCTGTGCCTTCCAGGGCAATGTGGTGGTGGG
  CAGGGACGTGTCGGTTCCAGAGCTTCGGGAAGCCTACCATGCTGTGGTGCTGAGTTATGGAGCAGAGGAC
  CACCAACCCCTGGGAATTCCTGGCGAGGAGCTGCCTGGAGTGGTCTCAGCCCGGGCCTTTGTGGGCTGGT
  ACAATGGACTTCCCGAGAACCAGGAGCTGGCGCCAGATCTGAGCTGTGACACGGCTGTAATTCTGGGACA
  GGGGAATGTGGCTCTGGATGTGGCCCGGATCCTGCTGACCCCACCTGAGCACCTGGAGAAAACAGACATC
  ACAGAGGCTGCATTGGGGGCCCTGAGGCAGAGTCGGGTGAAGACTGTGTGGATAGTGGGCCGGCGTGGGC
  CCTTGCAAGTAGCGTTCACCATTAAGGAGCTTCGGGAGATGATTCAGTTGCCAGGAACCCGGCCCATTTT
  GGATCCTTCGGATTTCTTGGGCCTCCAGGACAGAATTAAGGATGTCCCCCGTCCAAGGAGGCGGCTAACA
  GAACTGCTGCTTCGGACAGCCACGGAGAAGCCAGGAGTGGAAGAGGCTGCCCGCCAGGCACTGGCCTCCC
  GGGCCTGGGGTCTCCGCTTTTTCCGAAGCCCCCAGCAGGTGCTCCCTACCCCAGATGGCCAACGGGTAGC
  AGGCATCCGCCTGGCAGTTACTAGTCTAGAGGGTGTTGGGGAGTCCACTCGGGCAGTGCCCACAGGAGAC
  GTGGAGGACCTCCCTTGTGGACTGCTGCTGAGCAGCGTTGGGTATAAGAGCCGCCCCATCGACCCCAGCG
  TGCCCTTTGACCCCAAGCTTGGAGTCATCCCCAACACAGAGGGCCGGGTTGTGAATGTCCCAGGCCTCTA
  CTGCAGTGGCTGGGTGAAGAGGGGACCCACAGGTGTCATCACCACAACCATGACAGACAGCTTCCTCACC
  AGCCAGGCGCTGCTGGAGGACCTGAAGGCGGGGCTGCTGCCCTCCGGCCCCAGACCCGGCTATGTGGCCA
  TTCAAGCCCTGCTCAGCAATCGAGGAGTCCGGCCAGTGTCTTTCTCAGACTGGGAGAAGCTGGATGCTGA
  GGAAGTCTCTCGAGGCCAAGGTACTGGGAAACCAAGGGAGAAGCTGGTGGATCGAAGAGAGATGCTGCGG
  CTGCTGGGCCACTGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_007997
• Insert Size: 1485 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_007997.1, NP_032023.1
• RefSeq Size: 1866 bp
• RefSeq ORF: 1485 bp
• Locus ID: 14149
• UniProt ID: Q61578
• Cytogenetics: 11 E2
• Gene Summary: Serves as the first electron transfer protein in all the mitochondrial P450 systems including cholesterol side chain cleavage in all steroidogenic tissues, steroid 11-beta hydroxylation in the adrenal cortex, 25-OH-vitamin D3-24 hydroxylation in the kidney, and sterol C-27 hydroxylation in the liver.[UniProtKB/Swiss-Prot Function]