P2rx2 (NM_153400) Mouse Untagged Clone

CAT#: MC216721

P2rx2 (untagged) - Mouse purinergic receptor P2X, ligand-gated ion channel, 2 (P2rx2), transcript variant 1, (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: P2rx2
• Synonyms: P2x2; P2X2a
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >NM_153400.4
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCCGCTGCACAGCCCCGGCTTCCCGCGGGGGCGGCCATGGTCCGGCGCTTGGCCCGGGGCTGCTGGT
  CCGCGTTCTGGGACTACGAGACGCCCAAGGTGATCGTGGTGCGGAATCGGCGCCTGGGATTCGTGCACCG
  CATGGTGCAGCTGCTCATTCTGCTTTACTTCGTGTGGTACGTCTTCATCGTGCAGAAAAGCTACCAGGAT
  AGCGAAACCGGTCCGGAGAGCTCCATCATCACCAAAGTCAAGGGGATCACCATGTCGGAACACAAAGTGT
  GGGACGTGGAGGAATACGTAAAGCCCCCGGAGGGGGGCAGTGTAGTCAGCATCATCACCAGGATCGAGGT
  TACTCCTTCCCAGACCCTGGGAACATGCCCAGAGAGCATGAGGGTTCACAGCTCTACCTGCCATTTAGAT
  GACGACTGTGTGGCCGGACAGCTGGACATGCAGGGCAATGGGATTCGGACAGGACGCTGTGTACCCTATT
  ACCATGGGGACTCCAAGACCTGCGAGGTGTCAGCCTGGTGCCCGGTGGAGGATGGGACTTCTGAAAACCA
  TTTTCTGGGTAAAATGGCCCCAAATTTCACCATCCTCATCAAGAACAGCATCCACTATCCCAAGTTCAAG
  TTCTCCAAGGGCAACATTGCAAGCCAGAAGAGTGACTACCTGAAGCACTGCACGTTTGATCAGGACTCTG
  ATCCATACTGTCCCATCTTCAGGCTGGGCTTCATTGTAGAGCAAGCAGGAGAGAACTTCACAGAACTGGC
  ACACAAGGGCGGTGTCATTGGGGTCATCATCAACTGGAACTGTGACCTGGACTTGTCTGAATCAGAGTGC
  AACCCCAAATATTCTTTCCGGAGGCTCGACCCCAAGTATGACCCTGCCTCTTCAGGCTACAACTTCAGGT
  TTGCCAAATATTACAAGATAAACGGCACCACCACCACTCGAACTCTCATCAAAGCCTATGGGATTCGAAT
  TGACGTTATTGTGCATGGACAGGCAGGGAAATTCAGTCTCATTCCCACCATCATCAATCTGGCCACTGCT
  CTGACCTCCATCGGGGTGGGCTCCTTTCTGTGTGACTGGATTTTGTTAACGTTCATGAACAAAAACAAGC
  TCTACAGCCATAAGAAGTTCGACAAGGTGCGTACCCCAAGGCACCCCTCAAGTAGATGGCCTGTGACCCT
  TGCCCTTGTCTTGGGCCAGATCCCTCCCCCACCTAGTCACTACTCCCAGGACCAGCCACCCAGCCTTCCA
  TCAGGTGAAGGACCAGCTTTGGGAGAAGGGGCAGAGCTACCACTGGCTGTCCAGCCTCCTCGGTCTTGCT
  CCAGCTCTGCTCTGACTGAGCAGGTGGTGGACACTCTTGACCAGCATATGGGACAAAGGCCTCCTGTCCC
  TGAGCCTTCCCAACAGGACTCCACATCCACGGACCCCAAAGGTTTGGCCCAACTTTGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCTGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_153400
• Insert Size: 1458 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_153400.4, NP_700449.2
• RefSeq Size: 1919 bp
• RefSeq ORF: 1458 bp
• Locus ID: 231602
• UniProt ID: Q8K3P1
• Cytogenetics: 5 F
• Gene Summary: Ion channel gated by extracellular ATP involved in a variety of cellular responses, such as excitatory postsynaptic responses in sensory neurons, neuromuscular junctions (NMJ) formation, hearing, perception of taste and peristalsis. In the inner ear, regulates sound transduction and auditory neurotransmission, outer hair cell electromotility, inner ear gap junctions, and K(+) recycling. Mediates synaptic transmission between neurons and from neurons to smooth muscle.[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (1) encodes isoform a.