Gpr37l1 (NM_134438) Mouse Untagged Clone

CAT#: MC216704

Gpr37l1 (untagged) - Mouse G protein-coupled receptor 37-like 1 (Gpr37l1), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Gpr37l1
• Synonyms: AW047233; CAG-18; D0Kist8
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC216704 representing NM_134438
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCGGTGGCTGTGGCCCCTGGCTGTGTCTCTTGTTGTAGTGTTGACTGTGGGGCTGAGTGGGGTCTCTG
  GGGCTGCCACCTCATCTTTAGGTGGGCATAGAGCCAAGGTCCAGGAGCAGCAGAGTCGACCCCGAAGAGG
  CACCAAGGACGAGGGGCCCAAGGAGGTACAGCACTATGTACCCGAGGAGTGGGCTGAATATCCCAAGCCC
  ATCCATCCTGCTGGCCTGCAGCCCACCAAGACTTTGGAGGCCACTAGCCCCAACCCAGACAAGGATGGGG
  CCACCCCAGGTAATGGACAAGAGCTGAGGGTCAACCTGACCGGGACGCCAAGTCAGAGGCTGCAGATTCA
  GAACCCCCTGTATCCGGTGACCGAGAGCTCCTACAGCGCCTACGCCATCATGCTCCTGGCTCTGGTGGTG
  TTTGCTGTGGGTATCGTTGGCAATCTGTCTGTCATGTGCATTGTGTGGCACAGCTACTACTTGAAGAGTG
  CCTGGAACTCTATCCTTGCCAGCCTGGCTCTCTGGGATTTCCTGGTCCTCTTCTTCTGCCTCCCAATTGT
  CATCTTCAATGAGATTACCAAGCAAAGGCTACTCGGGGATGTTTCTTGCCGGGCAGTGCCCTTCATGGAG
  GTCTCCTCCCTGGGAGTCACAACCTTCAGTCTCTGTGCCCTGGGCATAGACCGATTCCATGTGGCCACCA
  GCACCCTGCCAAAGGTGAGGCCCATTGAGCGATGTCAATCAATTCTGGCTAAACTAGCTGTCATCTGGGT
  GGGCTCCATGATGCTGGCCGTGCCCGAACTCCTGCTATGGCAGCTGGCCCAGGAGCCGGCTCCCACCGCA
  GGAACTGTGGACTCGTGTATCATGAAACCTTCAGCTGACCTGCCCGAATCTGTCTATTCGCTGGTGATGA
  CCTACCAGAATGCCCGCATGTGGTGGTATTTTGGTTGCTACTTCTGTCTGCCCATCCTCTTCACCGTCAC
  CTGTCAGCTGGTGACGTGGCGGGTGCGGGGCCCACCAGGCAGGAAGCCTGAGTGTAGAGCGGGCAGGCAT
  GAGCAGTGTGAGAGCCAGCTCAACAGCACCGTGGTCGGCCTGACTGTGGTCTATGCCTTCTGTACACTCC
  CTGAGAACGTCTGCAATATCGTGGTGGCTTACCTCTCTACCGAGCTCACGCGGCAGACCCTGGACCTCCT
  GGGCCTCATCAACCAGTTCTCCACCTTCTTTAAGGGCGCCATCACCCCTGTGCTCCTCCTGTGCATCTGC
  AGGCCACTGGGCCAGGCCTTTCTGGATTGCTGCTGCTGCTGCTGCTGCGAGGAGTGCGGCGGGGCCTCAG
  ACTCCTCAGCCACAGTCAGCGCGGACAGCAAGCTGAAGGCGGAAGTGTCCTCTTCTATCTACTTCCACAA
  GCCCAGGGAGTCGCCCCCACTCCTGCCCCTGGGCACCCCTTGCTGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_134438
• Insert Size: 1446 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_134438.3, NP_602320.2
• RefSeq Size: 2267 bp
• RefSeq ORF: 1446 bp
• Locus ID: 171469
• UniProt ID: Q99JG2
• Cytogenetics: 1 E4
• Gene Summary: G-protein coupled receptor (PubMed:27072655). Has been shown to bind the neuroprotective and glioprotective factor prosaposin (PSAP), leading to endocytosis followed by an ERK phosphorylation cascade (By similarity). However, other studies have shown that prosaposin does not increase activity (By similarity). It has been suggested that GPR37L1 is a constitutively active receptor which signals through the guanine nucleotide-binding protein G(s) subunit alpha (By similarity). Participates in the regulation of postnatal cerebellar development by modulating the Shh pathway (PubMed:24062445). Regulates baseline blood pressure in females and protects against cardiovascular stress in males (PubMed:29625592). Mediates inhibition of astrocyte glutamate transporters and reduction in neuronal N-methyl-D-aspartate receptor activity (PubMed:28795439).[UniProtKB/Swiss-Prot Function]