Traf4 (NM_009423) Mouse Untagged Clone

CAT#: MC216484

Traf4 (untagged) - Mouse TNF receptor associated factor 4 (Traf4), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Traf4
• Synonyms: A530032M13Rik; CART1; msp2
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC216484 representing NM_009423
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCCCGGCTTCGACTACAAGTTCCTGGAGAAGCCCAAGCGGCGGCTGCTGTGCCCACTGTGCGGGAAGC
  CCATGCGCGAGCCTGTGCAGGTGTCTACTTGTGGCCACCGCTTCTGCGACACCTGCCTGCAGGAGTTTCT
  CAGTGAAGGAGTCTTCAAATGCCCTGAGGACCAACTTCCTCTGGACTATGCCAAGATCTACCCAGACCCA
  GAGCTGGAGGTCCAGGTGTTAGGCTTGGCTATCCGCTGCATCCACAGTGAGGAGGGCTGCCGCTGGAGCG
  GGCCACTTCGTCATCTACAGGGCCACTTGAACACTTGCAGCTTCAATGTAGTCCCCTGCCCTAATCGCTG
  CCCAGCCAAGCTGAGCCGGCGTGACCTGCCTGCCCACCTGCAGCACGACTGCCCTAAGCGCCGCCTCAAG
  TGTGAATTCTGTGGCTGTGACTTCAGTGGGGAGGCCTACGAGAGTCACGAGGGCGTGTGCCCCCAAGAGA
  GTGTGTACTGTGAGAACAAGTGTGGGGCCCGCATGATGAGGCGACTGTTGGCCCAGCATGCCACGTCTGA
  GTGCCCCAAGCGCACCCAGCCCTGTGCCTACTGTACCAAGGAATTTGTTTATGACACCATCCAGAGCCAC
  CAGTACCAGTGCCCACGCCTGCCTGTTCCCTGCCCCAACCAATGTGGAGTGGGCACTGTGGCCCGGGAAG
  ACCTGCCCACCCATCTGAAGGATAGCTGCAGCACTGCCTTTGTTCTCTGCCCTTTCAAAGAGTCTGGCTG
  CAAGCACAGGTGCCCTAAGCTGGCAATGGGACGTCACGTGGAGGAGAGCGTAAAGCCACATCTGGCTATG
  ATGTGTGCCCTGGTGAGCCGACAGCGGCAAGAGCTGCAGGAGCTGCGAAGAGAGCTGGAAGAACTATCCA
  TAGGCAGCGATGGAGTGCTCATCTGGAAGATTGGCAGCTATGGGCGTCGTCTACAAGAGGCCAAGGCCAA
  GCCTAACCTGGAGTGCTTCAGCCCAGCCTTCTATACGCATAAGTACGGCTACAAGCTGCAGGTGTCTGCA
  TTCCTCAACGGCAACGGCAGTGGTGAGGGCACACACCTCTCCATCTACATCCGTGTCTTGCCGGGCGCCT
  TTGACAATCTCCTTGAGTGGCCCTTTGCCCGCCGGGTCACCTTCTCCCTGCTGGATCAGAGTGACCCAGG
  TTTGGCTAAGCCACAGCATGTCACTGAGACCTTCCACCCTGATCCAAACTGGAAGAACTTCCAGAAGCCC
  GGCACGTGGCGAGGCTCCCTGGATGAGAGTTCTCTGGGCTTCGGCTACCCCAAGTTCATTTCCCACCAGG
  ACATCCGAAAGCGGAACTACGTGCGGGATGATGCGGTTTTCATCCGTGCCTCTGTCGAACTGCCCCGGAA
  GATCCTCAGCTGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_009423
• Insert Size: 1413 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_009423.4, NP_033449.2
• RefSeq Size: 3016 bp
• RefSeq ORF: 1413 bp
• Locus ID: 22032
• UniProt ID: Q61382
• Cytogenetics: 11 B5
• Gene Summary: Adapter protein and signal transducer that links members of the tumor necrosis factor receptor (TNFR) family to different signaling pathways. Plays a role in the activation of NF-kappa-B and JNK, and in the regulation of cell survival and apoptosis. Regulates activation of NF-kappa-B in response to signaling through Toll-like receptors. Required for activation of RPS6KB1 in response to TNF signaling. Modulates TRAF6 functions (By similarity). Required for normal skeleton development, and for normal development of the respiratory tract.[UniProtKB/Swiss-Prot Function]