Tnfrsf1b (NM_011610) Mouse Untagged Clone

CAT#: MC216483

Tnfrsf1b (untagged) - Mouse tumor necrosis factor receptor superfamily, member 1b (Tnfrsf1b), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Tnfrsf1b
• Synonyms: CD120b; p75; TNF-alphaR2; TNF-R-II; TNF-R2; TNF-R75; TNFalpha-R2; TNFBR; Tnfr-1; Tnfr2; TNFR80
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC216483 representing NM_011610
  Red=Cloning site Blue=ORF
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCGCCCGCCGCCCTCTGGGTCGCGCTGGTCTTCGAACTGCAGCTGTGGGCCACCGGGCACACAGTGC
  CCGCCCAGGTTGTCTTGACACCCTACAAACCGGAACCTGGGTACGAGTGCCAGATCTCACAGGAATACTA
  TGACAGGAAGGCTCAGATGTGCTGTGCTAAGTGTCCTCCTGGCCAATATGTGAAACATTTCTGCAACAAG
  ACCTCGGACACCGTGTGTGCGGACTGTGAGGCAAGCATGTATACCCAGGTCTGGAACCAGTTTCGTACAT
  GTTTGAGCTGCAGTTCTTCCTGTACCACTGACCAGGTGGAGATCCGCGCCTGCACTAAACAGCAGAACCG
  AGTGTGTGCTTGCGAAGCTGGCAGGTACTGCGCCTTGAAAACCCATTCTGGCAGCTGTCGACAGTGCATG
  AGGCTGAGCAAGTGCGGCCCTGGCTTCGGAGTGGCCAGTTCAAGAGCCCCAAATGGAAATGTGCTATGCA
  AGGCCTGTGCCCCAGGGACGTTCTCTGACACCACATCATCCACTGATGTGTGCAGGCCCCACCGCATCTG
  TAGCATCCTGGCTATTCCCGGAAATGCAAGCACAGATGCAGTCTGTGCGCCCGAGTCCCCAACTCTAAGT
  GCCATCCCAAGGACACTCTACGTATCTCAGCCAGAGCCCACAAGATCCCAACCCCTGGATCAAGAGCCAG
  GGCCCAGCCAAACTCCAAGCATCCTTACATCGTTGGGTTCAACCCCCATTATTGAACAAAGTACCAAGGG
  TGGCATCTCTCTTCCAATTGGTCTGATTGTTGGAGTGACATCACTGGGTCTGCTGATGTTAGGACTGGTG
  AACTGCATCATCCTGGTGCAGAGGAAAAAGAAGCCCTCCTGCCTACAAAGAGATGCCAAGGTGCCTCATG
  TGCCTGATGAGAAATCCCAGGATGCAGTAGGCCTTGAGCAGCAGCACCTGTTGACCACAGCACCCAGTTC
  CAGCAGCAGCTCCCTAGAGAGCTCAGCCAGCGCTGGGGACCGAAGGGCGCCCCCTGGGGGCCATCCCCAA
  GCAAGAGTCATGGCGGAGGCCCAAGGGTTTCAGGAGGCCCGTGCCAGCTCCAGGATTTCAGATTCTTCCC
  ACGGAAGCCACGGGACCCACGTCAACGTCACCTGCATCGTGAACGTCTGTAGCAGCTCTGACCACAGTTC
  TCAGTGCTCTTCCCAAGCCAGCGCCACAGTGGGAGACCCAGATGCCAAGCCCTCAGCGTCCCCAAAGGAT
  GAGCAGGTCCCCTTCTCTCAGGAGGAGTGTCCGTCTCAGTCCCCGTGTGAGACTACAGAGACACTGCAGA
  GCCATGAGAAGCCCTTGCCCCTTGGTGTGCCGGATATGGGCATGAAGCCCAGCCAAGCTGGCTGGTTTGA
  TCAGATTGCAGTCAAAGTGGCCTGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_011610
• Insert Size: 1425 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: BC141405, AAI41406
• RefSeq Size: 4137 bp
• RefSeq ORF: 1425 bp
• Locus ID: 21938
• UniProt ID: P25119
• Cytogenetics: 4 78.17 cM
• Gene Summary: Receptor with high affinity for TNFSF2/TNF-alpha and approximately 5-fold lower affinity for homotrimeric TNFSF1/lymphotoxin-alpha. The TRAF1/TRAF2 complex recruits the apoptotic suppressors BIRC2 and BIRC3 to TNFRSF1B/TNFR2 (By similarity).[UniProtKB/Swiss-Prot Function]