Chia1 (NM_023186) Mouse Untagged Clone

CAT#: MC216422

Chia (untagged) - Mouse chitinase, acidic (Chia), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Chia1
• Synonyms: 2200003E03Rik; AMCase; Chia; YNL
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC216422 representing NM_023186
  Red=Cloning site Blue=ORF
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCCAAGCTACTTCTCGTCACAGGTCTGGCTCTTCTGCTGAATGCTCAGCTGGGGTCTGCCTACAATC
  TGATATGCTATTTCACCAACTGGGCCCAGTATCGGCCAGGTCTGGGGAGCTTCAAGCCTGATGACATTAA
  CCCCTGCCTGTGTACTCACCTGATCTATGCCTTTGCTGGGATGCAGAACAATGAGATCACCACCATAGAA
  TGGAATGATGTTACTCTCTATAAAGCTTTCAATGACTTGAAAAACAGGAACAGCAAACTGAAAACCCTCC
  TGGCAATTGGAGGCTGGAACTTTGGAACTGCTCCTTTCACTACCATGGTTTCCACTTCTCAGAACCGCCA
  GACCTTCATTACCTCAGTCATCAAATTTCTGCGTCAGTATGGGTTTGATGGACTGGACCTGGACTGGGAA
  TACCCAGGCTCACGTGGGAGCCCTCCTCAGGACAAGCATCTCTTCACTGTCCTGGTGAAGGAAATGCGTG
  AAGCTTTTGAGCAGGAGGCTATTGAGAGCAACAGGCCCAGACTGATGGTTACTGCTGCTGTAGCTGGTGG
  GATTTCCAACATCCAGGCTGGCTATGAGATCCCTGAACTTTCTAAGTACCTGGATTTCATCCATGTCATG
  ACATATGACCTCCATGGCTCCTGGGAGGGCTACACTGGGGAGAATAGTCCTCTTTACAAATACCCTACTG
  AGACTGGTAGCAATGCCTACCTCAATGTGGATTATGTCATGAACTATTGGAAGAACAATGGAGCCCCAGC
  TGAGAAGCTCATTGTTGGATTCCCAGAGTATGGACACACCTTCATCCTGAGAAACCCCTCTGATAATGGA
  ATTGGTGCCCCTACCTCTGGTGATGGCCCTGCTGGGCCCTATACCAGACAGGCTGGGTTCTGGGCCTACT
  ATGAGATTTGCACCTTTCTGAGAAGTGGAGCCACTGAGGTCTGGGATGCCTCCCAAGAAGTGCCCTATGC
  CTATAAGGCCAACGAGTGGCTTGGCTATGACAATATCAAGAGCTTCAGTGTTAAGGCTCAGTGGCTTAAG
  CAGAACAATTTTGGAGGTGCCATGATCTGGGCCATTGACCTTGATGACTTCACTGGCTCTTTCTGTGATC
  AGGGAAAATTTCCTCTGACTTCTACTTTGAACAAAGCCCTTGGCATATCCACTGAAGGTTGCACAGCTCC
  TGACGTGCCTTCCGAGCCAGTGACTACTCCTCCAGGAAGTGGGAGTGGGGGTGGAAGCTCCGGAGGAAGC
  TCTGGAGGCAGTGGATTCTGTGCCGACAAAGCAGATGGCCTCTACCCTGTGGCAGATGACAGAAATGCTT
  TTTGGCAGTGCATCAATGGAATCACATACCAGCAGCATTGTCAAGCAGGGCTTGTTTTTGATACCAGCTG
  TAATTGCTGCAACTGGCCATGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_023186
• Insert Size: 1422 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: BC034548, AAH34548
• RefSeq Size: 1557 bp
• RefSeq ORF: 1422 bp
• Locus ID: 81600
• UniProt ID: Q91XA9
• Cytogenetics: 3 F2.2
• Gene Summary: Degrades chitin and chitotriose. May participate in the defense against nematodes, fungi and other pathogens. Plays a role in T-helper cell type 2 (Th2) immune response. Contributes to the response to IL-13 and inflammation in response to IL-13. Stimulates chemokine production by pulmonary epithelial cells. Protects lung epithelial cells against apoptosis and promotes phosphorylation of AKT1. Its function in the inflammatory response and in protecting cells against apoptosis is inhibited by allosamidin, suggesting that the function of this protein depends on carbohydrate binding. Presence in saliva and gastric juice suggests a function as a digestive enzyme.[UniProtKB/Swiss-Prot Function]