Mief1 (NM_178719) Mouse Untagged Clone

CAT#: MC216374

Smcr7l (untagged) - Mouse Smith-Magenis syndrome chromosome region, candidate 7-like (human) (Smcr7l), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Mief1
• Synonyms: A230016E22; AI452372; Smcr7l
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC216374 representing NM_178719
  Red=Cloning site Blue=ORF
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCAGGCGCTGGTGAGCGCAAAGGGAAGAAGGATGACAATGGCATTGGTACGGCCATCGATTTTGTGC
  TCTCCAATGCCCGGCTGGTGCTGGGTGTGGGTGGAGCAGCTATGTTGGGCATCGCCACACTGGCAGTTAA
  GCGGATGTATGACCGGGCAATCAGTGCCCCTACCAGCCCTACCCGCCTAAGCCATTCAGGGAAGAGGAGC
  TGGGAGGAGCCAAACTGGATGGGCTCTCCTCGCCTATTGAACAAGGACATGAAGGCAGGCCTGAGCCGGT
  CCCTGCAGACCCTTCCCACAGACTCTTCTGCCTTTGACACAGATACATTCTGCCCACCCCGGCCCAAACC
  ATTGGCCAGGAGGGGCCAGGTAGACTTGAAGAAGTCACGACTCCGCATGTCCCTGCAGGAGAAACTTCTT
  TCTTACTACCGGAACCGGGCAGCCATCCCTGCTGGAGAGCAGGCTCGGGCCAAGCAAGCTGCGGTGGACA
  TATGTGCTGAGCTCCGGAGCTTCCTGCGGGCCAAGCTGCCTGATATGCCACTTCGGGACATGTACTTGAG
  TGGCAGCCTCTATGATGATCTGCAGGTGGTGACGGCTGACCATATCCAACTCATTGTCCCCCTTGTGCTG
  GAGCAGAACCTGTGGTCATGCATACCTGGGGAGGACACCATCATGAATGTCCCTGGTTTCTTCCTGGTTC
  GTCGTGAGAACCCAGAGTACTTTCCTCGTGGTAGCAGTTATTGGGACCGATGTGTTGTAGGTGGCTACCT
  CTCCCCAAAGACAGTGGCAGACACCTTTGAGAAAGTGGTGGCAGGCTCCATCAACTGGCCAGCCATAGGG
  TCCCTCTTAGACTATGTGATCCGACCAGCACCACCCCCAGAGGCCCTGACACTAGAAGTTCAGTATGAGA
  AGGACAAACATCTTGTCATTGACTTCCTGCCATCAGTAACCCTTGGTGACACTGTCTTGGTGGCCAGACC
  ACACCGGTTAGCTCAGTATGACAACCTGTGGCGGCTGAGCCTTCGTCCTGCTGAGACAGCACGCTTACGG
  GCTTTGGACCAGGCGGACTCCGGCTGCCGGTCTCTTTGCCTCAAGATCCTCAAGGCCATATGCAAGTCCA
  CTCCAGCTCTGGGCCACCTCACTGCCAGCCAGCTAACCAACGTCATCCTCCACTTGGCCCAGGAGGAGGC
  TGACTGGTCTCCAGACATGTTGGCTGACCGCTTTCTACAGGCCCTGAGGGGACTCATCAGCTACTTGGAG
  GCTGGGGTCTTGCCCAGTGCCCTGAACCCTAAGGTGAACCTATTTGCAGAGCTCACCCCTCAAGAAATAG
  ACGAGTTGGGATATACCCTCTACTGCTCACTGTCTGAGCCGGAGGTGCTGCTGCAGACGTAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_178719
• Insert Size: 1392 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: BC137633, AAI37634
• RefSeq Size: 3281 bp
• RefSeq ORF: 1392 bp
• Locus ID: 239555
• UniProt ID: Q8BGV8
• Cytogenetics: 15 E1
• Gene Summary: Mitochondrial outer membrane protein which regulates mitochondrial fission. Promotes the recruitment and association of the fission mediator dynamin-related protein 1 (DNM1L) to the mitochondrial surface independently of the mitochondrial fission FIS1 and MFF proteins. Regulates DNM1L GTPase activity and DNM1L oligomerization. Binds ADP and can also bind GDP, although with lower affinity. Does not bind CDP, UDP, ATP, AMP or GTP. Inhibits DNM1L GTPase activity in the absence of bound ADP. Requires ADP to stimulate DNM1L GTPase activity and the assembly of DNM1L into long, oligomeric tubules with a spiral pattern, as opposed to the ring-like DNM1L oligomers observed in the absence of bound ADP. Does not require ADP for its function in recruiting DNM1L.[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (2) differs in the 5' UTR compared to variant 1. Both variants 1 and 2 encode the same protein. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.