Rasgef1c (NM_029004) Mouse Untagged Clone

CAT#: MC216336

Rasgef1c (untagged) - Mouse RasGEF domain family, member 1C (Rasgef1c), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Rasgef1c
• Synonyms: 9130006A14Rik
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC216336 representing NM_029004
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCCCCGGACACTGACTGCCTCCGACATGGTCACCCCAGGCAGCCTCAGCCCGCCTCCCACAGAATCCA
  CAGAAGGAGAGCAGGCCGGGCAGCCCCTCCTGGACGGAGCTCCATCCTCAGCCTCTTTGGACACCCTGAT
  CCAACACCTCGTGCCCACAGCCGATTACTATCCCGAGAAAGCCTACATCTTCACCTTCCTGCTGAGCTCT
  CGTCTCTTCATCGAGCCCCGAGAGCTCCTGGCTCGGGTGTGTCACCTGTGCATAGAGCAGCAACAGCTGG
  ACAAGCCGGTGCTGGACAAGGCCCGAGTCCGGAAGTTTGGTGCCAAATTGCTCCAGTTGTTAGCCGAGTG
  GACTGAGACCTTCCCACGGGACTTTGAAGAGGAGTCCACCATTGGACACCTGACAGACGTGGTGGGCCGC
  ATCTCCCCGTGTGACGAGACCTACGGGAGCAGGGTACACCAGCTCCTGCAGACTCTGCACCAGAAATTGG
  CGTCACTTGGCCAGGGACCAGAAAGCCTGGTGGGAGCCGACAAGCCCATCTCCTACAGGACCAAGCCTCC
  AGCCTCCATCCACAGGGAGCTCCTGGGTGTCTGCAGTGACCCCTACACGCTGGCCCAGCAACTGACCCAC
  GTGGAGCTGGAGCGACTAAGGCACATTGGGCCCGAGGAGTTTGTCCAGGCTTTTGTGAACAAGGACCCCC
  TGGCAGGCACAAAGCCCCGCTTCAGTGACAAGACGAACAACGTTGAGGCCTACGTGAAGTGGTTCAACAG
  ACTATGCTACCTGGTAGCAACGGAAATCTGCATGCCAGCCAAGAAGAAGCAGAGAGCGCAGGTGATCGAG
  TTCTTCATCGATGTGGCCCGAGAGTGCTTCAACATCGGCAACTTCAACTCCCTCATGGCCATTATCTCTG
  GCATGAACATGAGCCCTGTCTCCAGGCTGAAGAAGACTTGGGCCAAAGTGAAAACAGCCAAATTCTTCAT
  TCTGGAGCACCAGATGGACCCAACAGGGAACTTCTGCAACTATAGGACAGCCCTGCGTGGGGCAGCCCAC
  CGCTCACTCACTGCTCACAGCAGCAGGGAGAAGATCGTCATCCCCTTCTTCAGTCTGCTCATCAAGGACA
  TTTACTTCCTCAATGAGGGCTGTGCCAACCGCCTTCCCAATGGGCATGTCAACTTTGAGAAATTTCTGGA
  ACTGGCCAAGCAGGTTGGAGAATTCATTACTTGGAAACAAGTGGAATGTCCTTTCGAGCAAGATCCGAGC
  ATCACCCATTACCTGTACACTGCCCCCATCTTCAGCGAAGACGGTTTGTATTTGGCTTCCTATGAAAGCG
  AGAGCCCAGAGAGCCAGACAGAAAAGGAGAGGTGGAAATCTCTAAGATCTTCTATTCTGGGGAAGACATG
  A
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_029004
• Insert Size: 1401 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_029004.1, NP_083280.1
• RefSeq Size: 2248 bp
• RefSeq ORF: 1401 bp
• Locus ID: 74563
• UniProt ID: Q9D300
• Cytogenetics: 11 B1.3
• Gene Summary: Guanine nucleotide exchange factor (GEF).[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (2) differs in the 5' UTR, lacks a portion of the 5' coding region and initiates translation at a downstream start codon, compared to variant 1. It encodes isoform 2, which has a shorter N-terminus, compared to isoform 1.