Ppp2r5e (NM_012024) Mouse Untagged Clone

CAT#: MC216297

Ppp2r5e (untagged) - Mouse protein phosphatase 2, regulatory subunit B (B56), epsilon isoform (Ppp2r5e), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Ppp2r5e
• Synonyms: 4633401M22Rik; AI449017; B56beta
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC216297 representing NM_012024
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTCCTCAGCACCAACTACTCCTCCATCAGTGGATAAAGTAGACGGATTTTCTCGGAAGTCCGTCAGAA
  AAGCCAGACAGAAGAGGTCGCAAAGCTCCTCCCAGTTCAGGTCTCAAGGCAAGCCCATTGAGCTCACGCC
  TCTGCCACTGCTGAAAGACGTTCCAACCTCAGAGCAGCCTGAACTGTTCCTAAAGAAACTTCAGCAGTGC
  TGTGTCATTTTTGACTTCATGGACACGCTATCTGATCTTAAAATGAAAGAATACAAGCGCTCCACTCTTA
  ATGAACTGGTGGACTACATTACAATAAGCAGAGGCTGTCTGACAGAGCAGACTTACCCTGAAGTAGTTAG
  AATGGTATCTTGCAATATATTCAGAACTCTCCCTCCTAGTGACAGCAATGAATTCGACCCAGAAGAAGAT
  GAACCTACCCTCGAGGCATCATGGCCCCACTTACAGCTTGTATATGAATTTTTCATAAGATTTTTGGAAA
  GCCAAGAATTCCAACCCAGCATTGCCAAAAAATACATAGATCAGAAATTTGTATTACAGCTTTTGGAGCT
  ATTTGACAGTGAAGACCCTCGAGAACGGGACTATTTAAAAACAGTCTTGCACAGGATTTATGGCAAGTTT
  CTCGGGCTTAGAGCATTTATCCGAAAACAAATTAACAATATTTTTCTAAGGTTTGTTTATGAAACAGAAC
  ACTTCAATGGTGTAGCTGAACTGCTGGAAATATTAGGAAGTATTATCAATGGCTTTGCTTTACCACTCAA
  GGCAGAACACAAACAGTTCCTGGTGAAAGTGTTGATCCCTCTACACACTGTCAGGAGCTTGTCACTCTTT
  CATGCACAGCTGGCGTATTGTATAGTACAGTTTCTGGAGAAAGACCCTTCCCTTACAGAACCAGTTATTA
  GGGGGCTAATGAAATTTTGGCCTAAAACATGCAGTCAAAAAGAGGTCATGTTCCTCGGGGAGCTGGAAGA
  AATCTTGGATGTGATTGAACCGTCACAATTTGTTAAAATCCAAGAACCTTTGTTTAAACAAATTGCCAAG
  TGTGTTTCTAGCCCCCATTTTCAGGTGGCAGAAAGGGCACTCTATTATTGGAATAATGAATACATCATGA
  GTTTGATAGAAGAAAACTCTAACGTCATCCTTCCCATCATGTTCTCCAGCCTTTATAGGATTTCAAAAGA
  GCATTGGAATCCGGCCATTGTGGCATTGGTGTACAACGTGTTGAAGGCATTTATGGAAATGAACAGCACT
  ATGTTTGATGAGCTGACAGCCACATACAAGTCAGATCGTCAGCGTGAGAAAAAGAAAGAAAAGGAGCGAG
  AAGAACTGTGGAAAAAATTGGAGGATCTGGAGTTAAAGAGAGGTCTTAGACGCGATGGGATAATTCCAAC
  TTAA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_012024
• Insert Size: 1404 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_012024.2, NP_036154.1
• RefSeq Size: 4841 bp
• RefSeq ORF: 1404 bp
• Locus ID: 26932
• UniProt ID: Q61151
• Cytogenetics: 12 C3
• Gene Summary: The B regulatory subunit might modulate substrate selectivity and catalytic activity, and also might direct the localization of the catalytic enzyme to a particular subcellular compartment. Interacts with cyclin G in vitro.[UniProtKB/Swiss-Prot Function]