Eef1a2 (NM_007906) Mouse Untagged Clone

CAT#: MC216235

Eef1a2 (untagged) - Mouse eukaryotic translation elongation factor 1 alpha 2 (Eef1a2), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Eef1a2
• Synonyms: Eef1a; EEF1AL; S1; STN; wasted; wst
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC216235 representing NM_007906
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGGCAAGGAGAAGACACACATCAACATTGTGGTCATTGGCCACGTGGACTCAGGCAAGTCCACCACGA
  CAGGCCACCTCATCTACAAGTGTGGTGGCATCGACAAGCGGACCATCGAGAAGTTTGAGAAGGAGGCAGC
  AGAGATGGGGAAGGGCTCTTTTAAATATGCCTGGGTGCTGGACAAGCTGAAGGCCGAGCGGGAACGAGGC
  ATCACCATCGACATCTCCCTCTGGAAGTTTGAGACCACCAAGTACTACATCACCATCATCGATGCTCCAG
  GACACCGAGACTTCATCAAGAATATGATTACAGGCACATCCCAGGCGGACTGCGCAGTGCTGATCGTGGC
  AGCCGGTGTGGGCGAGTTTGAGGCGGGCATCTCCAAGAACGGGCAAACCCGGGAACACGCACTCCTGGCC
  TACACTCTGGGTGTGAAGCAGCTCATTGTGGGTGTCAACAAGATGGACTCCACGGAACCAGCCTACAGCG
  AGAAGCGCTATGATGAGATTGTTAAGGAGGTCAGCGCCTACATCAAGAAGATCGGCTACAACCCAGCCAC
  GGTGCCCTTCGTGCCCATCTCGGGCTGGCATGGTGACAACATGCTGGAGCCTTCACCTAATATGCCATGG
  TTCAAGGGCTGGAAAGTAGAGCGTAAGGAAGGAAATGCAAGCGGCGTGTCCCTGCTGGAAGCCCTGGACA
  CAATCCTGCCCCCCACCCGCCCCACTGACAAGCCCCTTCGTCTGCCTCTGCAGGATGTGTACAAGATTGG
  GGGCATTGGGACCGTGCCTGTGGGCCGAGTGGAGACCGGTATCCTCCGGCCTGGTATGGTGGTGACCTTT
  GCGCCAGTCAACATCACCACAGAGGTGAAGTCTGTGGAAATGCACCATGAGGCACTTAGCGAGGCCCTGC
  CTGGTGACAATGTCGGGTTCAATGTGAAGAATGTGTCCGTTAAGGATATTCGCCGGGGCAATGTCTGCGG
  GGACAGCAAAGCTGACCCGCCTCAGGAGGCTGCCCAGTTCACCTCTCAGGTTATCATCCTGAACCACCCT
  GGGCAAATCAGCGCTGGCTACTCGCCAGTCATCGACTGTCACACGGCCCACATTGCCTGCAAGTTTGCCG
  AGCTAAAGGAGAAGATTGACCGTCGTTCTGGCAAGAAGCTGGAGGATAACCCCAAGTCCCTGAAGTCTGG
  TGATGCAGCCATTGTTGAGATGGTCCCTGGAAAACCCATGTGTGTGGAGAGCTTCTCACAGTACCCACCT
  CTCGGCCGCTTCGCCGTGCGCGACATGCGGCAGACTGTGGCCGTGGGCGTCATCAAGAACGTGGAGAAGA
  AGAGCGGCGGCGCAGGCAAGGTCACCAAGTCCGCACAGAAGGCTCAGAAAGCGGGCAAGTGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_007906
• Insert Size: 1392 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_007906.2, NP_031932.1
• RefSeq Size: 2111 bp
• RefSeq ORF: 1392 bp
• Locus ID: 13628
• UniProt ID: P62631
• Cytogenetics: 2 103.6 cM
• Gene Summary: This protein promotes the GTP-dependent binding of aminoacyl-tRNA to the A-site of ribosomes during protein biosynthesis.[UniProtKB/Swiss-Prot Function]