Manea (NM_172865) Mouse Untagged Clone

CAT#: MC216233

Manea (untagged) - Mouse mannosidase, endo-alpha (Manea), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Manea
• Synonyms: 4932703L02Rik
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC216233 representing NM_172865
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCAAAATTTCGAAGAAGGACCTGCATCCTTTTGTCACTTTTTATTCTATTTATTTTTTCTCTGATGA
  TGGGCTTAAAGATGCTGTGGCCAAACGCAGCATCCTTTGGACCTCCTTTTGGACTTGACCTCCTTCCAGA
  ACTTCATCCACTAAATGCGCATTCGGGAAACAAAGCTGACTTCCAAAGGAGTGATAGAATCAACATGGAA
  ACAAACACCAAGGCTTTAAAAGGCGCTGGCATGACTGTGCTGCCAGCCAAAGCCTCTGAGGTGAACCTGG
  AAGAACTACCTCCTCTGAATTACTTTTTACATGCATTTTATTACAGTTGGTATGGAAATCCACAGTTTGA
  TGGTAAATATATACACTGGAATCATCCGGTCCTGGAACACTGGGACCCTCGGATAGCCAAGAACTATCCA
  CAAGGACAACATAGTCCTCCAGACGACATTGGCTCCAGTTTTTATCCTGAGTTAGGAAGTTACAGCTCTC
  GAGACCCTTCTGTCATAGAAACTCACATGAAACAAATGCGCTCAGCCTCAATTGGAGTTCTGGCCCTGTC
  TTGGTACCCACCTGATTCAAGGGATGACAATGGCGAAGCTACTGATCACTTGGTGCCAACCATTTTGGAT
  AAAGCTCATAAATATAATCTGAAGGTCACTTTTCACATAGAGCCATATAGCAATCGAGATGATCAAAACA
  TGCATCAAAATATCAAGTATATTATAGACAAATATGGAAACCATCCAGCCTTTTATAGATACAAGACCAG
  GACTGGGCATTCTCTGCCCATGTTTTATGTCTATGATTCTTACATCACAAAGCCTACAATATGGGCCAAT
  CTGTTAACACCCTCCGGATCTCAGAGTGTTCGCAGTTCTCCTTATGATGGATTGTTTATTGCACTTCTAG
  TAGAAGAAAAGCATAAAAATGATATTCTTCAGAGTGGTTTTGATGGTATTTACACATATTTTGCCACAAA
  TGGCTTTACATATGGCTCATCTCATCAGAATTGGAATAACCTGAAATCCTTTTGTGAAAAGAACAACTTG
  ATGTTTATCCCAAGTGTAGGCCCAGGATACATAGATACAAGCATCCGACCATGGAACACTCAGAACACCC
  GGAACAGAGTCAATGGGAAGTATTATGAAGTTGGTCTAAGTGCTGCACTCCAGACCCACCCCAGTTTAAT
  TTCCATCACCTCTTTCAATGAGTGGCATGAAGGAACTCAAATTGAAAAGGCTGTCCCCAAAAGAACTGCT
  AACACGATATACCTGGATTACCGGCCTCATAAGCCAAGTCTTTATCTAGAACTAACTCGAAAGTGGTCTG
  AAAAATTCAGTAAGGAAAGAATGACGTATGCATTGGATCAACAGCAGCCTGCTTCATAA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_172865
• Insert Size: 1389 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_172865.2, NP_766453.2
• RefSeq Size: 4645 bp
• RefSeq ORF: 1389 bp
• Locus ID: 242362
• UniProt ID: Q6NXH2
• Cytogenetics: 4 A3