Ero1lb (NM_026184) Mouse Untagged Clone

CAT#: MC216227

Ero1lb (untagged) - Mouse ERO1-like beta (S. cerevisiae) (Ero1lb), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Ero1lb
• Synonyms: 1300013B24Rik; 1700065B09Rik; AI447560; ero1-beta; Ero1b
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC216227 representing NM_026184
  Red=Cloning site Blue=ORF
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAGTCCGGGGTTCCGCCGGGCCGTTACTGGGCAGGGGGCGGCGGCCGCGGTGCAACTGCTTGTCACCC
  TGAGCTTCCTCTCAAGTCTGGTCAAGACTCAGGTGACTGGAGTTCTGGATGATTGCTTATGTGACATTGA
  CAGCATTGATAAATTCAACACCTACAAAATCTTTCCCAAAATAAAGAAGTTACAAGAACGAGACTATTTT
  CGTTATTACAAGGTTAATCTGAAACGACCATGTCCTTTCTGGGCAGAAGATGGCCACTGCTCAATAAAAG
  ACTGTCATGTGGAGCCCTGTCCAGAAAGTAAAATTCCAGTTGGAATTAAAGCCGGGCGTTCAAATAAGTA
  CTCGCAAGCAGCAAACAGCACCAAAGAACTGGATGACTGTGAGCAGGCTAACAAACTGGGCGCCATCAAC
  AGCACGCTAAGTAACGAAAGCAAAGAAGCGTTCATTGACTGGGCGAGATATGATGATTCGCAGGACCACT
  TTTGTGAACTTGATGATGAGCGGTCTCCTGCTGCACAGTATGTGGACCTGCTGCTGAACCCGGAACGGTA
  CACTGGCTACAAGGGCTCCTCAGCATGGAGGGTGTGGAACAGCATCTATGAAGAAAACTGCTTCAAGCCT
  CGATCTGTTTATCGTCCTTTAAATCCTTTGGCGCCCAGCAGAGGGGAAGATGATGGAGAATCATTCTATA
  CGTGGCTAGAAGGTTTGTGTCTTGAGAAAAGAGTCTTCTATAAGCTTATATCAGGACTCCATGCCAGCAT
  CAATTTACATCTGTGTGCAAACTACCTTCTGGAAGAAACCTGGGGGAAACCTAGTTGGGGACCAAACATC
  AAGGAGTTTAGACGCCGCTTTGACCCTGTGGAAACAAAGGGGGAAGGTCCAAGGAGGCTAAAGAACCTGT
  ACTTTTTATACTTGATAGAGCTCCGTGCTTTGTCAAAGGTGGCCCCTTACTTTGAGCGCTCGATTGTTGA
  TCTCTATACTGGCAATGTGGAAGATGATGCCGACACCAAGACCCTTCTGCTCAGCATCTTTCAGGATACA
  AAGTCCTTTCCTATGCACTTCGATGAGAAATCCATGTTTGCAGGTGACAAAAAGGGGGCCAAGTCATTAA
  AGGAAGAATTCCGGTTACATTTCAAGAACATCTCCCGGATCATGGACTGTGTTGGGTGCGATAAATGCAG
  ACTGTGGGGGAAACTGCAGACTCAGGGTTTAGGAACTGCCTTGAAGATCCTCTTCTCTGAAAAGGAAATC
  CAAAACCTTCCGGAGAACAGCCCATCCAAAGGCTTCCAGCTCACTCGGCAGGAAATCGTTGCTCTTTTAA
  ATGCTTTTGGAAGACTTTCTACAAGCATAAGAGAATTACAGAACTTTAAAGCGTTGTTGCAGCACAGGAG
  GTAA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_026184
• Insert Size: 3 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: BC058721, AAH58721
• RefSeq Size: 4255 bp
• RefSeq ORF: 1404 bp
• Locus ID: 67475
• UniProt ID: Q8R2E9
• Cytogenetics: 13 A1
• Gene Summary: Oxidoreductase involved in disulfide bond formation in the endoplasmic reticulum. Efficiently reoxidizes P4HB/PDI, the enzyme catalyzing protein disulfide formation, in order to allow P4HB to sustain additional rounds of disulfide formation. Other protein disulfide isomerase family members can also be reoxidized, but at lower rates compared to P4HB, including PDIA2, PDIA3, PDIA4, PDIA6 and NXNDC12. Following P4HB reoxidation, passes its electrons to molecular oxygen via FAD, leading to the production of reactive oxygen species (ROS) in the cell (By similarity). Involved in oxidative proinsulin folding in pancreatic cells, hence required for glucose homeostasis in vivo.[UniProtKB/Swiss-Prot Function]