Gdf6 (NM_013526) Mouse Untagged Clone

CAT#: MC216201

Gdf6 (untagged) - Mouse growth differentiation factor 6 (Gdf6), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Gdf6
• Synonyms: BMP-13; BMP1; BMP13; GDF16
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC216201 representing NM_013526
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGACACTCCTAGGGTCCTTCTCTGGGCGATCTTCCTCATTAGCTTCCTCTGGGATTTGCCGGGTTTCC
  AGCAGGCTTCCATCTCCTCGTCCTCCTCCTCCTCCACCGAATTGGACTCCACCAAAGACGTGGGGAACCG
  CAAAGAAGGGAAGATGCAGCGAACTCCACAAGAGAGTGCCGAGGGCCGGACGCCCCCGGAGCACGGGCTG
  CGGCAGAAGGACCTCCGGCGGCGGCCGCCCGGACAACATCAGGGGCAGGAGCCGCCGGGCAGGGGGCTGC
  GCGTGGTGCCTCACGAGTACATGCTGTCAATCTACAAGACTTACTCCATTGCCGAGAAGCTGGGCATCAA
  TGCCAGCTTTTTCCAGTCTTCCAAGTCAGCTAATACGATCACTAGCTTTGTAGACAGAGGACTGGACGAT
  CTCTCGCACACTCCTCTCCGGAGACAGAAGTATTTGTTTGATGTGTCCACACTCTCAGACAAAGAAGAGC
  TGGTGGGCGCAGAGCTAAGGCTTTATCGCCAGGCGCCCCCAACGCCCTGGGGGCTACCGGCCCGACCGCT
  GCACTTGCAGCTCTTCCCTTGTTTATCCCCATTGCTACTGGACGCCAGGACCCTGGATCCTCAGGGACCA
  ACCCAGGCCGGCTGGGAAGTCTTCGACGTGTGGCAGGGCCTGCGCCCTCAGCCTTGGAAGCAGCTGTGCC
  TGGAGTTGCGGGCAGCCTGGGGTGAGCTGGACGCCGGGGATACGGGGGCGCGCGCGAGGGGTCCCCAGCA
  GCCACCGCCTCTGGACCTGCGGAGTCTGGGCTTCGGTCGGAGGGTGAGGCCGCCCCAGGAGCGCGCCCTG
  CTTGTAGTGTTCACCAGATCGCAGCGCAAGAACCTGTTCACTGAGATGCATGAGCAGCTGGGCTCTGCAG
  AGGCTGCGGGAGCCGAGGGGTCATGGCCAGCGCCGTCGGGCTCCCCAGACGCCGGGTCTTGGCTGCCCTC
  GCCCGGCCGCCGGCGGCGACGCACCGCCTTCGCCAGCCGTCACGGCAAGCGACATGGCAAGAAGTCCAGG
  CTGCGCTGCAGCAGAAAGCCTCTGCACGTGAATTTTAAGGAGTTAGGCTGGGACGACTGGATTATCGCGC
  CCCTAGAGTACGAGGCCTATCACTGCGAGGGCGTGTGCGACTTTCCGCTGCGCTCGCACCTTGAGCCCAC
  TAACCATGCCATCATTCAGACGCTGATGAACTCCATGGACCCGGGCTCCACCCCGCCTAGCTGCTGCGTT
  CCCACCAAACTGACTCCCATTAGCATCCTGTACATCGACGCGGGCAATAATGTAGTCTACAAGCAGTATG
  AGGACATGGTGGTGGAGTCCTGCGGCTGTAGGTAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_013526
• Insert Size: 1365 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_013526.1, NP_038554.1
• RefSeq Size: 3532 bp
• RefSeq ORF: 1365 bp
• Locus ID: 242316
• UniProt ID: P43028
• Cytogenetics: 4 A1
• Gene Summary: This gene encodes a secreted ligand of the TGF-beta (transforming growth factor-beta) superfamily of proteins. Ligands of this family bind various TGF-beta receptors leading to recruitment and activation of SMAD family transcription factors that regulate gene expression. The encoded preproprotein is proteolytically processed to generate each subunit of the disulfide-linked homodimer. This protein is required for normal formation of some bones and joints in the limbs, skull, and axial skeleton. Mice lacking a functional copy of this gene exhibit joint and skeletal defects. [provided by RefSeq, Sep 2016]