Ush1g (NM_176847) Mouse Untagged Clone

CAT#: MC216119

Ush1g (untagged) - Mouse Usher syndrome 1G homolog (human) (Ush1g), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Ush1g
• Synonyms: j; js; Sans
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC216119 representing NM_176847
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAATGACCAGTATCACCGGGCCGCCAGGGATGGCTATCTGGAACTCCTCAAGGAGGCCACCCGGAAGG
  AGCTGAATGCCCCTGACGAGGATGGCATGACCCCGACTCTCTGGGCTGCCTACCATGGCAACCTGGAGTC
  TCTGCGCCTCATTGTGAGCCGCGGGGGTGACCCGGATAAGTGTGACATCTGGGGAAACACACCCTTGCAT
  CTGGCAGCTTCCAATGGTCACCTGCACTGCCTGTCCTTCCTCGTCTCCTTCGGGGCCAACATCTGGTGCC
  TGGACAATGACTACCACACGCCACTGGACATGGCCGCTATGAAGGGCCACATGGAGTGCGTACGCTATCT
  GGATTCCATCGCGGCCAAGCAGAGCAGCCTCAACCCCAAGCTGGTGGGCAAGCTGAAGGACAAGGCCTTC
  CGCGAGGCAGAGCGGCGCATCCGCGAGTGCGCCAAGATGCAGCGCAAGCACCACGAGCGCATGGAGCGGC
  GCTACCGGCGCGAGCTGGCCGAGCGCTCCGACACTCTCAGCTTCTCCAGCCTCACGTCCAGCACCCTGAG
  CCGACGGCTGCAGCACATGACGCTGGGCAGCCAGCTGCCCTACTCGCAGGCCACGCTGCACGGTACGGCC
  AAGGGCAAGGCCAAGATCCAGAAGAAGCTGGAGAGGCGCAAGCAGGGGGGCGAGGGCACCTTCAAGGTCT
  CTGAGGACGGGCGCAAAAGCGTCCGATCGCTCTCCGGCCTGCAGTTGGGTAGTGATGTGATGTTTGTGCG
  CCAGGGCACCTACGCCAACCCCAAGGAGTGGGGCCGTGCCCCACTCAGGGACATGTTCCTCTCGGACGAG
  GACAGCGTCTCCCGTGCCACACTGGCTGCCGAGCCTGCTCACTCGGAGGTCAGCACCGACTCAGGCCACG
  ACTCTTTGTTTACCCGCCCCGGCCTGGGTACCATGGTGTTTCGAAGGAACTATGTGAGCAGCGGGTTGCA
  CGGGCTGGGCCGAGAGGACGGGGGTTTGGATGGGGCAGGCACGCCGCGGGGTCGGCTGCATAGTTCCCCC
  AGCCTGGACGACGACAGCCTAGGCAGTGCCAACAGCTTGCAGGACCGCAGTTGCGGGGAAGAGCTGCCCT
  GGGATGAGCTAGACTTGGGCTTGGATGAGGACCTGGAGCCCGAGACCAGCCCCTTGGAGACCTTCCTGGC
  CTCGCTGCACATGGAAGACTTTGCCTCTCTCCTGCGGCACGAGAAGATTGACCTGGAGGCTCTGATGCTG
  TGCTCTGACCTGGACCTGCGCAGCATCAGCGTGCCCCTGGGGCCTCGGAAGAAGATCCTGGGGGCCGTGA
  GGAGGCGCAGGCAGGCTCTGGAGCGACCCCTGGCCCTGGAGGACACGGAGCTATGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_176847
• Insert Size: 1386 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_176847.3, NP_789817.1
• RefSeq Size: 3183 bp
• RefSeq ORF: 1386 bp
• Locus ID: 16470
• UniProt ID: Q80T11
• Cytogenetics: 11 80.84 cM
• Gene Summary: This gene encodes a protein that contains three ankyrin domains, a class I PDZ-binding motif and a sterile alpha motif. The encoded protein interacts with harmonin, which is associated with Usher syndrome type 1C. This protein plays a role in the development and maintenance of the auditory and visual systems and functions in the cohesion of hair bundles formed by inner ear sensory cells. Mutations in this gene are associated with combined auditory and vision loss. [provided by RefSeq, Sep 2015]