Rnf25 (NM_021313) Mouse Untagged Clone

CAT#: MC216086

Rnf25 (untagged) - Mouse ring finger protein 25 (Rnf25), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Rnf25
• Synonyms: 0610009H16Rik; AO7
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC216086 representing NM_021313
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCGGCGTCTGCGTCGACAGCTGCTGGGGAAGAGGACTGGGTCCTTCCCTCTGAAGTTGAAGTATTAG
  AGTCTATCTATCTGGATGAGCTACAGGTGATGAAAGGGAATGGCAGATCACCATGGGAGATCTTTATCAC
  TCTGCACCCTGCCACCGCAGAGGTCCAAGATTCGCAGTTTGTCTGCTTTACTCTGGTTCTTCGGATCCCA
  GTCCAGTATCCCCACGAGGTTCCACAGATCTCCATCCGTAACCCTCGAGGACTTTCAGATGAGCAGATCC
  ACAAGATCTCACAGGCCCTGGGCCATGTGGCCAAGGAAGGACTAGGCACTGCCATGCTCTATGAACTCAT
  TGAGAAAGGGAAGGAGATTCTCACGGATAACAACATTCCCCATGGCCAGTGTGTCATCTGCCTCTATGGT
  TTCCAGGAGAAGGAGGCCTTTACCAAAACACCCTGTTACCACTACTTCCACTGCCATTGCCTTGCCAGGT
  ACATCCAGCACATGGAACAAGAGCTGACTACACAGGAACAGGAACAGGAGCGACAGCACGTTGTCACCAA
  GCAGAAGGCAGTCGGTGTGCAGTGTCCTGTGTGCAGGGAGCCCCTTGTGTATGATCTTGCCTCGCTGAAA
  GCAGCCCCTGAACCCCAACAACCCATGGAGCTGTACCAACCCAGTGCAGAGAGCTTGCGCCAACAGGAAG
  AGCTCAAACTGCTGTATCAGAGGCAGCAGGAAAAGGGTGGTATCATTGACCTTGAGGCTGAACGGAACCG
  ATACTTCATTAGCCTTCAGCAGCCTCCTGCTGCTCTTGAGCCGGAGTCAGCTGTAGATGTCTCCAGAGAA
  CCCCAGCCACCTAATGCCCTTTCTGCAGAACAATCTACTTCCCTAGCTGACCAATCAACTCTGCCAACTT
  CTCTACCCATGACAACCCAGTATACGTATGAGAAGACTTCGGGGGCTGGGCCAAATCAACAGAGGCCGGG
  TGAGACCCAGAAATCCGTGCTAGATCCTCCACGACATGGTCGTGGCTCCTGGCGACAGTATGATCGGAGG
  CACCCAAAAGGAGGGGAGTGCTGCACCCCAAAAGGTACCAGTGAAATCCATGAACTGCCACCTCCTGAGA
  AGCCTCTCAAGGAGACTGTAGACTTAAAGGCAGAACCCCGTAACAAAGGGCTCACAGGTCATCCTCAGGA
  GAAGGGGCCTGGTAGCTGGCAGGGCCCCTCAGCTCGCAGGACTCGGGACTGTGCTCGCTGGGAACGCTCC
  AAAAACCGTACACCGGGTTCTTGCTATCCCCACCTACCTCGGGGCCAGGGAGCATACCGGTCTGGTACTC
  GCAGGGAGCCCCTGGGCTTGGAATCTGAGGAAGGTTCCTAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_021313
• Insert Size: 1371 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_021313.3, NP_067288.2
• RefSeq Size: 1498 bp
• RefSeq ORF: 1371 bp
• Locus ID: 57751
• UniProt ID: Q9QZR0
• Cytogenetics: 1 C3
• Gene Summary: E3 ubiquitin-protein ligase that mediates ubiquitination and subsequent proteasomal degradation of NKD2. Stimulates transcription mediated by NF-kappa-B (By similarity).[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1).