Krt27 (NM_010666) Mouse Untagged Clone

CAT#: MC215948

Krt27 (untagged) - Mouse keratin 27 (Krt27), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Krt27
• Synonyms: CK-27; Krt1-c2; Krt1-c29; mIRS; mIRSa3.1; st; stpm
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC215948 representing NM_010666
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTCCGTTCGCTTTTCTTCCGCATCCAGACGGCTGGGCTCTGTGAGGCTCTCCAGCGCGGGAGCAGCCT
  TGGGGGCTGGAAACGCATGCGGAGTGCCGGGCATCGGAAGCGGCTTCTCCTGCGCCTTTGGGGGCAGTTC
  ATTGGCAGGGGGGCTCGGTATGGGCGGTGCTTCTTGTGGGGCCTTCACAGCGAATGAGCACGGCCTCCTC
  TCCGGCAATGAGAAGGTCACCATGCAGAACCTTAATGACCGCCTGGCCTCCTACCTGGAGAACGTGCAAG
  CACTGGAGGAGGCCAATGCTGACCTGGAGCAGAAGATCAAGGACTGGTATGAAAAATTTGGACCTGGGTC
  GTGCCGCGGTCTCGATCATGACTACAGTAGATACTTCCCCATCATTGATGACCTCAGGACCCAGATCATC
  TCTGCAACTGCACACAACGCCAACATCATCCTGCAAAATGACAACGCAAGACTGACGGCGGATGACTTCA
  GAATGAAGTATGAGAATGAACTGGCACTCCACCAGAGCGTCGATGCTGACATCAATGGCTTGCGGAGGGT
  CCTGGATGAGCTGACCCTGTGCAGAACCGACCTTGAGGTCCAGCTGGAAACACTCAGTGAGGAGCTGGCA
  TACCTCAAGAAGAACCACGAGGAGGAAATGCAGGCTCTGCAGTGCGCCGCTGGAGGCAACGTGAACGTGG
  AGATGAACGCAGCCCCGGGCGTGGACCTCACCGTCCTGCTGAACAACATGAGAGCCGAGTACGAGGCCCT
  GGCTGAGCAGAACCGTAGGGACGCCGAGGCCTGGTTCCAAGAGAAGAGCGCCTCCCTGCAGCAGCAGATT
  TCAGACGATGCTGGAGCCACCACCTCAGCCAGGAACGAACTTACAGAGATGAAACGGACTCTCCAAACCC
  TGGAGATAGAGCTACAGTCCCTCTTAGCCATGAAACACTCTCTGGAGTGCTCCCTGACGGAGACCGAGGG
  CAACTACTGCACGCAGCTGGCTCAGATCCAGGCTCAGATCAGCGCCCTGGAGGAGCAGCTGCACCAAGTG
  AGGACGGAGACGGAGGGCCAGAAGCTGGAATATGAGCAGCTGCTGAATGTCAAGGCCCACCTGGAGAAGG
  AGATCGAGACCTACTGCCGCCTCATAGATGGAGACGAGGGCTCTTGTGTTAAGGCAAAAGGCCAAGGAAG
  ACCCGGAAATCAGACAAAAGATTCGCCTAAAACTGCCATCGTTAAAACTGTCGTGGAAGAGTTAGACCCC
  CGTGGCAAGGTTCTCTCCTCCAGAGTTCACACCCTGGAAGAGAAATCCACCAAAGTCAACAAGACCGAGC
  AGAGGATCCCTTCTTGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_010666
• Insert Size: 1347 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_010666.2, NP_034796.1
• RefSeq Size: 1570 bp
• RefSeq ORF: 1347 bp
• Locus ID: 16675
• UniProt ID: Q9Z320
• Cytogenetics: 11 62.92 cM
• Gene Summary: This gene encodes a member of the type I (acidic) keratin family, which belongs to the superfamily of intermediate filament (IF) proteins. Keratins are heteropolymeric structural proteins which form the intermediate filament. These filaments, along with actin microfilaments and microtubules, compose the cytoskeleton of epithelial cells. The mouse type I keratin genes are clustered in a region of chromosome 11. The encoded protein is involved in the formation of intermediate filaments in the inner root sheath. [provided by RefSeq, Sep 2015]