Gpat3 (NM_172715) Mouse Untagged Clone

CAT#: MC215874

Agpat9 (untagged) - Mouse 1-acylglycerol-3-phosphate O-acyltransferase 9 (Agpat9), transcript variant 1, (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Gpat3
• Synonyms: 1-AGPAT; 1-AGPAT 9; 4933407I02Rik; 4933408F15; A230097K15Rik; GPAT-3; Gpat3; mGPAT3
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC215874 representing NM_172715
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAGGGCGCAGACCTGGCGGTGAAGCTCCTGTCCACCTGGCTGACGCTGGTGGGCGGCCTCATCCTTT
  TACCCTCGGCCTTCGGATTATCCCTGGGTATCTCGGAGATCTACATGAAGATCCTGGTGAAAACCTTGGA
  GTGGGCCACATTACGAATTCAGAAAGGAGCCCCCAAGGAGTCAGCTCTTAAAAACTCAGCTTCTGTGGGT
  ATTATCCAAAGAGATGAGTCACCCATGGAGAAAGGGCTCTCTGGTCTTCGAGGAAGGGACTTCGAGCTCT
  CTGATGTGTTCTATTTCTCCAAGAAGGGGTTGGAAGCCATTGTGGAGGATGAAGTGACCCAGAGGTTCTC
  CTCCGAAGAGCTAGTATCATGGAACCTCCTCACACGAACCAATGTCAACTTTCAGTACATCAGTCCAAGG
  CTCACCATGGTGTGGGTGCTGGGTGTCCTAGTGCGCTATTGCTTCCTGCTACCTCTGAGGGTCACCCTGG
  CTTTCATTGGGATCAGCTTGCTGATTATAGGAACTACACTGGTTGGCCAGCTTCCAGACAGCAGCCTCAA
  AAACTGGCTGAGTGAGCTGGTGCATCTGACCTGCTGCAGGATCTGTGTTCGGTCCCTATCTGGCACCATC
  CATTACCATAACAAGCAGTACAGACCCCAGAAGGGAGGTATCTGTGTCGCCAATCACACCTCTCCCATTG
  ATGTCCTAATCCTGGCCACCGATGGATGTTACGCCATGGTTGGGCAGGTTCACGGTGGATTGATGGGGAT
  CATTCAGAGAGCCATGGTTAAGGCTTGTCCTCACGTCTGGTTTGAGCGCTCAGAAATAAAGGACAGACAT
  CTAGTGACTAAGAGATTAAAAGAACACATTGCTGACAAGAAAAAGTTACCCATTCTAATCTTCCCAGAAG
  GTACTTGCATCAACAATACTTCAGTCATGATGTTTAAAAAGGGAAGCTTTGAAATCGGAGGAACCATCTA
  TCCAGTGGCCATAAAGTATAACCCCCAGTTCGGCGATGCCTTCTGGAACAGTAGTAAATACAACTTGGTG
  AGCTACCTGCTTCGAATCATGACCAGCTGGGCCATTGTCTGCGACGTGTGGTACATGCCTCCCATGACTA
  GAGAGGAAGGAGAAGACGCAGTTCAGTTTGCAAACAGGGTAAAATCCGCTATCGCTGTACAAGGAGGGCT
  GACGGAACTTCCCTGGGATGGAGGGCTGAAGAGAGCAAAGGTGAAGGACACCTTCAAGGAAGAACAACAG
  AAGAATTACAGCAAGATGATCGTGGGCAACGGATCTCCCAACCTGGCGAGGGACTGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_172715
• Insert Size: 1317 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_172715.3, NP_766303.1
• RefSeq Size: 2877 bp
• RefSeq ORF: 1317 bp
• Locus ID: 231510
• UniProt ID: Q8C0N2
• Cytogenetics: 5 E4
• Gene Summary: Converts glycerol-3-phosphate to 1-acyl-sn-glycerol-3-phosphate (lysophosphatidic acid or LPA) by incorporating an acyl moiety at the sn-1 position of the glycerol backbone (PubMed:17170135). Also converts LPA into 1,2-diacyl-sn-glycerol-3-phosphate (phosphatidic acid or PA) by incorporating an acyl moiety at the sn-2 position of the glycerol backbone (By similarity).[UniProtKB/Swiss-Prot Function]